5-HT2B Antibody - BSA Free

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Analysis of anti-5HT2B Receptor antibody with human brain, cortex.

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

• Reactivity: Hu, Pm, Pm, Bv, Pm, Pm-Or
• Applications: Func, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

5-HT2B Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 15 amino acid peptide from 2nd extracellular domain of human 5-HT2B.
• Epitope: 2nd extracellular domain
• Localization: Cell Membrane
• Specificity: Human 5-HT2B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Bovine (93%), Orangutan (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Proximity Ligation Assay
• Application Notes: Use in Proximity ligation assay reported in scientific literature (PMID: 31091436). ICC: Does not work..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (80%), Hamster (80%), Equine (80%), Rabbit (80%), Porcine (80%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT2B Antibody - BSA Free

• 5-HT(2B)
• 5HT2B
• 5-HT2B
• 5-HT-2B
• 5-HT2B5-HT 2B receptor
• 5-hydroxytryptamine (serotonin) receptor 2B
• 5-hydroxytryptamine 2B receptor
• 5-hydroxytryptamine receptor 2B
• HTR2B
• Serotonin receptor 2B

Background

5-HT2B, a Serotonin Receptor, activates phospholipase C upon binding serotonin, which results in a rise in intracellular calcium. It is known that 5-HT2B regulates cardiovascular function during development and in adulthood; mice lacking functional 5-HT2B receptors died of heart defects during gestation or neonatally, and adult mutant mice displayed cardiopathy, including myocyte disarray and ventricular dilation. Abnormal vascular proliferation causes an increase in pulmonary blood pressure, which is associated with an increase in 5-HT2B receptor, resulting in the development of pulmonary hypertension. It has also been reported that the 5-HT2B is involved in cell-cycle regulation via interaction with tyrosine kinase pathways. 5-HT2B receptors may have a role in the initiation of migraine headaches, and selective antagonists may prove therapeutic in migraine treatment. 5-HT2B receptor is expressed in many tissues including human liver, kidney, lung, heart, pancreas, spleen, brain, spinal cord, gastrointestinal tract, placenta, and coronary and pulmonary arteries. It has also been reported in embryonic mouse heart and spinal cord. ESTs have been isolated from heart/melanocyte/uterus, kidney, prostate, skin, and thyroid libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT2B Antibody (NLS1111)(1)

Publication using NLS1111

• Traub LM Genetic and Functional Dissection of the Role of Individual 5-HT2 Receptors as Entry Receptors for JC Polyomavirus Cell Rep 2019-05-14 [PMID: 31091436] (PLA, Human)

Bioinformatics

• Gene Symbol: HTR2B
• Entrez:
  • 3357(Human)
• Uniprot:
  • P41595()