4EBP1 Overexpression Lysate (Native)


Western Blot: eIF4EBP1 Overexpression Lysate (Adult Normal) [NBL1-10206] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4EBP1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

4EBP1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201348 and based on accession number NM_004095. The protein contains a C-terminal DDK tag.
Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for 4EBP1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for 4EBP1 Overexpression Lysate (Native)

  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1


This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for 4EBP1 Lysate (NBL1-10206) (0)

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol EIF4EBP1

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