Recombinant Human 4-1BB/TNFRSF9/CD137 Protein

Images

 
SDS-Page: Human 4-1BB/TNFRSF9/CD137 Protein [NBP2-34958]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Human 4-1BB/TNFRSF9/CD137 Protein Summary

Description
A single, non-glycosylated polypeptide chain corresponding to 166 residues of 4-1BB/TNFRSF9/CD137.

Source: Escherichia coli

Amino Acid Sequence: ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
CD137 protein is fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 ug for both 4-1BB Ligand and 4-1BB Receptor.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFRSF9
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of rHu4-1BB Receptor as determined by LAL method.

Applications/Dilutions

Theoretical MW
17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human 4-1BB/TNFRSF9/CD137 Protein

  • 4-1BB ligand receptor
  • 41BB
  • 4-1BB
  • CD137 antigen
  • CD137
  • CD137MGC2172
  • CDw137
  • FLJ43501
  • ILA
  • ILAhomolog of mouse 4-1BB
  • induced by lymphocyte activation (ILA)
  • interleukin-activated receptor, homolog of mouse Ly63
  • receptor protein 4-1BB
  • T cell antigen ILA
  • T-cell antigen 4-1BB homolog
  • T-cell antigen ILA
  • TNFRSF9
  • tumor necrosis factor receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9

Background

4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
AF2295
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB342
Species: Hu
Applications: WB, AgAct
AF3388
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP2-25255
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
AF382
Species: Hu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, Neut
7270-IL/CF
Species: Hu
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC

Publications for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958) (0)

There are no publications for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958) (0)

There are no reviews for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional 4-1BB/TNFRSF9/CD137 Products

Bioinformatics Tool for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958)

Discover related pathways, diseases and genes to 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958)

Discover more about diseases related to 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958).
 

Pathways for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958)

View related products by pathway.

PTMs for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958)

Learn more about PTMs related to 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958).
 

Research Areas for 4-1BB/TNFRSF9/CD137 Protein (NBP2-34958)

Find related products by research area.

Blogs on 4-1BB/TNFRSF9/CD137

There are no specific blogs for 4-1BB/TNFRSF9/CD137, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human 4-1BB/TNFRSF9/CD137 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFRSF9
Uniprot