Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

Functional: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580] - Demonstration of the potency of mCD137L fusion protein in vitro in comparison to an agonist antibody 2A. Balb/c mouse splenocytes were incubated for 48 hrs on anti-CD3 (145-11C clone) monoclonal antibody coated plates in the presence of 2 mg/ml anti-CD3, 2A or Fc-mCD137L. The amount of IL-2 production induced by Fc-mCD137L was comparable to the induction by 2A antibody.

SDS-Page: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580] - Electrophoretic analysis of the purified Fc-mCD137L using Coomassie Blue stained 4-20% reducing SDS-PAGE.

• Reactivity: Mu
• Applications: Func, PAGE
• Concentration: 2.0 mg/ml

Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein Summary

• Description: A recombinant protein corresponding to TNFSF9.
  
  

  Amino Acid Sequence: EPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGKSGRTEPRPALTITTSPNLGTRENNADQVTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTTLNWHSQDGAGSSYLSQGLRYEEDKKELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVSLVLQAKPQVDDFDNLALTVELFPCSMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQDAYRDWELSYPNTTSFGLFLVKPDNPWE
  Mouse Ig-Fc amino acid sequences are in Italics
  Linker sequence (SGR) in bold
  Mouse CD137L sequences are underlined

• Details of Functionality: In vitro proliferation assay, in vivo assays.
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF9
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level < 0.1 EU/ug of the protein (< 0.01 ng/ug of the protein) as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized.
• Concentration: 2.0 mg/ml
• Purity: Protein A purified

Alternate Names for Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

• 41BB Ligand
• 4-1BB Ligand
• 4-1BBL
• 4-1BB-L
• CD137L
• homolog of mouse 4-1BB-L
• receptor 4-1BB ligand
• TNFSF9
• tumor necrosis factor (ligand) superfamily, member 9
• tumor necrosis factor ligand superfamily member 9

Background

CD137L is a member of the TNF superfamily and provides costimulatory signals to T cells. It is expressed on dendritic cells, activated macrophages, and B cells. CD137L binds to its receptor CD137 on activated T cells, this interaction is important for adaptive T-cell response. The engagement of CD137 by CD137L provides costimulation to both CD4+ and CD8+ cells. Agonist anti-CD137 monoclonal antibodies also have been used to stimulate CD137 to regulate T-cell number and functions. The Fc-CD137L fusion protein was generated by cloning the extracellular domain of mouse CD137L with the Fc region of mouse immunoglobulin (hinge region, CH2, CH3 domains). The Fc-CD137L fusion protein has comparable biological activities as 2A, a CD137 agonist antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for 4-1BB Ligand/TNFSF9 Recombinant Protein (NBP2-26580)(1)

Publication using NBP2-26580

• Zhang N, Sadun RE, Arias RS et al. Targeted and untargeted CD137L fusion proteins for the immunotherapy of experimental solid tumors. Clin Cancer Res. 2007-05-01 [PMID: 17460060]

Bioinformatics

• Gene Symbol: TNFSF9