4-1BB Ligand/TNFSF9 Antibody (2357A) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 2357A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

4-1BB Ligand/TNFSF9 Antibody (2357A) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB Ligand/TNFSF9 Antibody (2357A) [Alexa Fluor™ Plus 594]

• 41BB Ligand
• 4-1BB Ligand
• 4-1BBL
• 4-1BB-L
• CD137L
• homolog of mouse 4-1BB-L
• receptor 4-1BB ligand
• TNFSF9
• tumor necrosis factor (ligand) superfamily, member 9
• tumor necrosis factor ligand superfamily member 9

Background

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺ and CD8⁺ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
7. Baessler, T. et al. (2010) Blood 115:3058.
8. Gullo, C. et al. (2010) PLoS One 5:e10845.
9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
11. Wen, T. et al. (2002) J. Immunol. 168:4897.
12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.