4-1BB Ligand/TNFSF9 Antibody (203942) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
203942
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

4-1BB Ligand/TNFSF9 Antibody (203942) [Alexa Fluor® 350] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse 4‑1BB Ligand/TNFSF9
Arg104-Glu309
Accession # P41274
Specificity
Detects mouse 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human RELT is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TNFSF9
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB Ligand/TNFSF9 Antibody (203942) [Alexa Fluor® 350]

  • 41BB Ligand
  • 4-1BB Ligand
  • 4-1BBL
  • 4-1BB-L
  • CD137L
  • homolog of mouse 4-1BB-L
  • receptor 4-1BB ligand
  • TNFSF9
  • tumor necrosis factor (ligand) superfamily, member 9
  • tumor necrosis factor ligand superfamily member 9

Background

4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14‑16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart (1, 2). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines (3). A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases (4). By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules (3).

  1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Kwon, B., H.W. Lee and B.S Kwon (2002) TRENDS in Immunology 23:378.
  4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1246U
Alexa Fluor 405 FAB1246V
Alexa Fluor 488 FAB1246G
Alexa Fluor 594 FAB1246T
Alexa Fluor 647 FAB1246R
Alexa Fluor 700 FAB1246N
Alexa Fluor 750 FAB1246S

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Bioinformatics

Gene Symbol TNFSF9
Uniprot