Human 15-PGDH/HPGD ELISA Kit (Colorimetric)

ELISA: Human 15-PGDH/HPGD ELISA Kit (Colorimetric) [NBP2-80340] - Example standard curve for Human 15-PGDH/HPGD ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Human 15-PGDH/HPGD ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Human 15-PGDH/HPGD.
• Standard Curve Range: 54.69-3500 pg/ml (example only; lot dependent)
• Sensitivity: 19.2 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.5 %
• Intra-Assay: CV% < 3.2
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HPGD

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Human 15-PGDH/HPGD ELISA Kit (Colorimetric)

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 15-PGDH/HPGD ELISA Kit (NBP2-80340). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HPGD