| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21695-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99637-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59390-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58046-25ul | |||
![Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769] - Western blot analysis of extracts of various cell lines, using 14-3-3 zeta/beta Rabbit mAb (NBP3-16769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/14-3-3-zeta-beta-Antibody-3J3J9-Western-Blot-NBP3-16769-img0002.jpg "Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769] - Western blot analysis of extracts of various cell lines, using 14-3-3 zeta/beta Rabbit mAb (NBP3-16769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769] - Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 zeta/beta Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16769_rabbit-14-3-3-zeta-beta-mab-3j3j9-8220252010488.jpg)
![Western Blot: 14-3-3 zeta/beta Overexpression Lysate (Adult Normal) [NBP2-05475] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta/beta.](http://images.novusbio.com/fullsize/14-3-3-zeta-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05475-img0001.jpg "Western Blot: 14-3-3 zeta/beta Overexpression Lysate (Adult Normal) [NBP2-05475] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta/beta.")
![Western Blot: 14-3-3 zeta/beta Overexpression Lysate (Adult Normal) [NBP2-11092] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta/beta.](http://images.novusbio.com/fullsize/14-3-3-zeta-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11092-img0001.jpg "Western Blot: 14-3-3 zeta/beta Overexpression Lysate (Adult Normal) [NBP2-11092] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta/beta.")
![Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NB100-2140-img0001.jpg "Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.")
![Western Blot: 14-3-3 Antibody (SR1456) [NBP3-21695] - Western blot analysis using NBP3-21695 in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21695_rabbit-14-3-3-mab-sr1456-5720231564714.jpg "Western Blot: 14-3-3 Antibody (SR1456) [NBP3-21695] - Western blot analysis using NBP3-21695 in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate.")
![Western Blot: 14-3-3 Antibody [NBP2-99637] - Anti-14-3-3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa, Observed band size:26 kDa.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-99637-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-99637] - Anti-14-3-3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa, Observed band size:26 kDa.")
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0004.jpg "Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Immunocytochemistry-Immunofluorescence-NBP2-59390-img0002.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0006.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis in A431 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0001.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0005.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (S08-3F5) [NBP3-19942] - Western blot detection of PEDFR/PNPLA2/ATGL in K562 cell lysates using NBP3-19942 (1:500 diluted). Predicted band size: 55kDa. Observed band size: 55kDa.](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-S08-3F5-Western-Blot-NBP3-19942-img0001.jpg "Western Blot: PEDFR/PNPLA2/ATGL Antibody (S08-3F5) [NBP3-19942] - Western blot detection of PEDFR/PNPLA2/ATGL in K562 cell lysates using NBP3-19942 (1:500 diluted). Predicted band size: 55kDa. Observed band size: 55kDa.")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody [NBP2-58046] - Staining of human cell line A549 shows localization to nucleoplasm & lipid droplets.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58046-img0001.jpg "Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody [NBP2-58046] - Staining of human cell line A549 shows localization to nucleoplasm & lipid droplets.")