14-3-3 zeta Antibody (818515) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 818515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

14-3-3 zeta Antibody (818515) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human 14-3-3ζ
  Asp2-Asn245
  Accession # P63104
• Specificity: Detects human 14-3-3 zeta in direct ELISAs and Western blots. Detects Mouse and Rat 14-3-3 zeta in Western Blots. In direct ELISAs, no
  cross-reactivity with recombinant human 14-3-3 beta, theta, eta, gamma, sigma, or epsilon is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 zeta Antibody (818515) [Alexa Fluor® 488]

• 1433 zeta
• 14-3-3 zeta
• KCIP-1
• KCIP-1MGC126532,14-3-3-zeta
• MGC111427
• MGC138156
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
• YWHAZ
• zeta polypeptide

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha / beta , epsilon , eta , gamma , tau / theta , sigma , and zeta /δ). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (gene name YWHAZ) is a 245 amino acid, 27 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.