12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

Western Blot: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 12-Lipoxygenase.

Immunohistochemistry: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: OTI1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ALOX12 (NP_000688) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 75.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

• 12(S)-lipoxygenase
• 12-Lipoxygenase
• 12LO
• 12-LOX
• 12S-lipoxygenase
• 12S-LOX
• 12S-LOXarachidonate 12-lipoxygenase, 12S-type
• ALOX12
• arachidonate 12-lipoxygenase
• EC 1.13.11
• EC 1.13.11.-
• EC 1.13.11.31
• EC 1.13.11.33
• EC 3.3.2.-
• LOG12
• platelet 12-LOX
• platelet-type 12-lipoxygenase
• Platelet-type lipoxygenase 12

Background

Oxygenase and 14,15-leukotriene A4 synthase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALOX12