11 beta-HSD1 Antibody

Images

 
Western Blot: 11 beta-HSD1 Antibody [NBP1-32027] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: ...read more
Immunohistochemistry: 11 beta-HSD1 Antibody [NBP1-32027] - Paraffin-embedded hepatoma; antibody dilution 1:500.
Immunocytochemistry/ Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

11 beta-HSD1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSD11B1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD11B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 11 beta-HSD1 Antibody

  • 11 betaHSD1
  • 11 beta-HSD1
  • 11-beta-hydroxysteroid dehydrogenase 1
  • EC 1.1.1
  • EC 1.1.1.146
  • HDL
  • HSD11B1
  • HSD11HSD11B
  • HSD11LMGC13539
  • hydroxysteroid (11-beta) dehydrogenase 1
  • member 1
  • SDR26C1

Background

The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSD11B1
Entrez
Uniprot