11 beta-HSD1 Antibody

Western Blot: 11 beta-HSD1 Antibody [NBP1-32027] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP1-32027] - Hepatoma. HSD11B1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

11 beta-HSD1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 11 beta-HSD1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD11B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: IHC-FR- Assay dependent
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for 11 beta-HSD1 Antibody

• 11 betaHSD1
• 11 beta-HSD1
• 11-beta-hydroxysteroid dehydrogenase 1
• EC 1.1.1
• EC 1.1.1.146
• HDL
• HSD11B1
• HSD11HSD11B
• HSD11LMGC13539
• hydroxysteroid (11-beta) dehydrogenase 1
• member 1
• SDR26C1

Background

The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 11 beta-HSD1 Antibody (NBP1-32027)(1)

Publication using NBP1-32027

• Oleinik N, Kim J, Roth BM et al. Mitochondrial protein import is regulated by p17/PERMIT to mediate lipid metabolism and cellular stress Sci Adv 2019-09-01 [PMID: 31535025] (WB, Human)

Bioinformatics

• Gene Symbol: HSD11B1
• Entrez:
  • 3290(Human)
• Uniprot:
  • P28845()