Western Blot: 11 beta-HSD1 Antibody [NBP1-32027] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: ...read more
32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-32027 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 40% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified
Alternate Names for 11 beta-HSD1 Antibody
11 betaHSD1
11 beta-HSD1
11-beta-hydroxysteroid dehydrogenase 1
EC 1.1.1
EC 1.1.1.146
HDL
HSD11B1
HSD11HSD11B
HSD11LMGC13539
hydroxysteroid (11-beta) dehydrogenase 1
member 1
SDR26C1
Background
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for 11 beta-HSD1 Antibody (NBP1-32027)(1)
We have publications tested in 1 confirmed species: Human.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
![Western Blot: 11 beta-HSD1 Antibody [NBP1-32027] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/11-beta-HSD1-Antibody-Western-Blot-NBP1-32027-img0008.jpg "Western Blot: 11 beta-HSD1 Antibody [NBP1-32027] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/11-beta-HSD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32027-img0009.jpg "Immunocytochemistry/Immunofluorescence: 11 beta-HSD1 Antibody [NBP1-32027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry: 11 beta-HSD1 Antibody [NBP1-32027] - Paraffin-embedded hepatoma; antibody dilution 1:500.](http://images.novusbio.com/fullsize2/11-beta-HSD1-Antibody-Immunohistochemistry-NBP1-32027-img0007.jpg "Immunohistochemistry: 11 beta-HSD1 Antibody [NBP1-32027] - Paraffin-embedded hepatoma; antibody dilution 1:500.")
![Western Blot: HSD11B2 Antibody [NBP2-37898] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue](http://images.novusbio.com/images2/HSD11B2-Antibody-Western-Blot-NBP2-37898-img0002.jpg)
![Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human rectum.](http://images.novusbio.com/images2/HSD11B2 Antibody-Immunohistochemistry-Paraffin-NBP2-37898-img0008.jpg)
![Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human kidney shows high expression.](http://images.novusbio.com/images2/HSD11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-37898-img0003.jpg)
![Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Western-Blot-NB300-731-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Immunocytochemistry-Immunofluorescence-NB300-731-img0007.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/GR-NR3C1-Antibody-(BuGR2)-Flow-Cytometry-NB300-731-img0018.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-Paraffin-NB100-1533-img0003.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Western Blot: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Analysis of Mineralocorticoid Receptor.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Western-Blot-NB300-562-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Immunocytochemistry-Immunofluorescence-NB300-562-img0008.jpg)
![Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Immunohistochemistry-Paraffin-NB300-562-img0011.jpg)
![Western Blot: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glucose 1-dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glucose 1-dehydrogenase.](http://images.novusbio.com/images2/Glucose-1-dehydrogenase-Antibody-(2A7)-Western-Blot-NBP2-02615-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 1-dehydrogenase.](http://images.novusbio.com/images2/Glucose-1-dehydrogenase-Antibody-(2A7)-Immunocytochemistry-Immunofluorescence-NBP2-02615-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 1-dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/images2/Glucose-1-dehydrogenase-Antibody-(2A7)-Immunohistochemistry-Paraffin-NBP2-02615-img0016.jpg)
![Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Glutathione-Reductase-Antibody-Western-Blot-NBP2-16684-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Paraffin-embedded rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/images2/Glutathione-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-16684-img0005.jpg)
![Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/Glutathione Reductase Antibody-Western Blot-NBP2-16684-img0001.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).](http://images.novusbio.com/images2/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0010.jpg)
![Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.](http://images.novusbio.com/images2/COX-2-Antibody-Simple-Western-NB100-689-img0003.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/images2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Sample (30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/images2/Beta-Hydroxysteroid-Dehydrogenase-Antibody-Western-Blot-NBP1-32353-img0003.jpg)
![Immunohistochemistry-Paraffin: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Human normal liver. HSD3B2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/Beta-Hydroxysteroid-Dehydrogenase-Antibody-Immunohistochemistry-NBP1-32353-img0002.jpg)
![Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.](http://images.novusbio.com/images2/CYP11A1-Antibody-Western-Blot-NBP1-85368-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a verified customer review.](http://images.novusbio.com/images2/CYP11A1-Antibody-Immunofluorescence-NBP1-85368-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0007.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")