DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in
COMET™ Panel Builder." class="big_lightbox">

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in
COMET™ Panel Builder." alt="CD68 was detected in immersion fixed paraffin-embedded sections of mouse Liver using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog #MAB101141) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm. Protocol available in
COMET™ Panel Builder." class="big_lightbox">

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog #
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog #
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog #
FC004) and permeabilized with ice-cold methanol." class="big_lightbox">

MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog #
FC004) and permeabilized with ice-cold methanol." class="big_thumb" />