| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB101141-SP | |||
| Carrier Free | IC101141V-100UG | |||
| Carrier Free | IC101141U-100UG | |||
| Carrier Free | IC101141T-100UG | |||
| Carrier Free | IC101141S-100UG | |||
| Carrier Free | IC101141R-100UG | |||
| Carrier Free | IC101141N-100UG | |||
| Carrier Free | IC101141G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44491 | |||
| BSA Free | NBP2-34482-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70381 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44542 | |||
| Azide and BSA Free | NBP2-34662-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10114-SP | |||
| Carrier Free | IC10114V-100UG | |||
| Carrier Free | IC10114U-100UG | |||
| Carrier Free | IC10114T-100UG | |||
| Carrier Free | IC10114S-100UG | |||
| Carrier Free | IC10114R-100UG | |||
| Carrier Free | IC10114N-100UG | |||
| Carrier Free | IC10114G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47902-0.1mg | |||
| IHC-Prediluted | NBP2-48024 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44490 | |||
| BSA Free | NBP2-34481-25ul | |||
![Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CD68 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Western-Blot-NBP2-34482-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CD68 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human cerebellum shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0004.jpg)
![Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Lane 1: Marker [kDa]. Lane 2: Human spleen tissue lysate](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Western-Blot-NBP2-34482-img0005.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0001.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human liver shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human colon shows strong immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0003.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymph node tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0010.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymph node tissue.")
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0011.jpg)
![Western Blot: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Western-Blot-NBP2-46168-img0013.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0012.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0001.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0003.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0004.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0005.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0006.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0007.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0008.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human bladder tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0009.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (KP1 + C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-+-C68-684-Immunohistochemistry-Paraffin-NBP2-34329-img0008.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (KP1 + C68/684).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (KP1 + C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-+-C68-684-Immunohistochemistry-Paraffin-NBP2-34329-img0007.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-278202415295599.jpg "Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32163) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-298202414115445.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32163) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-29202410234233.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Flow cytometric analysis of THP-1 (positive) and Jurkat (negative) cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32163, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-392024884626.jpg)
![Western Blot: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Western-Blot-NBP2-44543-img0005.jpg "Western Blot: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (LAMP4/824).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Immunohistochemistry-Paraffin-NBP2-44543-img0004.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Flow Cytometric Analysis of U87MG cells using CD68/SR-D1 Antibody (LAMP4/824) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Flow-Cytometry-NBP2-44543-img0006.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Immunohistochemistry-Paraffin-NBP2-44543-img0003.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-278202415212467.jpg "Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32164) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-298202414161818.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32164) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-29202410234268.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29820241435295.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunocytochemistry analysis of THP-1 cells labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32162) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202410334075.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Flow cytometric analysis of THP-1 cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32162, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202416453272.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-278202415295560.jpg "Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-29820241433363.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32165) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-2920241048480.jpg)