Antibody News

In December 2009, researchers at the University of Southern California cited using Novus’ mouse monoclonal Telomerase Reverse Transcriptase Antibody (cat# NB100-317) [PMID: 19923445]. Specifically, Novus’ hTERT antibody was used for immunofluorescent staining on human gingiva-derived mesenchymal stem cells (GMSC). This hTERT specific antibody helped to demonstrate that the GMSC’s are in fact a new population of precursor cells “which exhibit several unique stem cell-like properties as MSCs derived from bone marrow and other postnatal tissues.”

...

C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been attributed to altered c-Myc expression.

In normal tissue c-Myc expression is tightly regulated, only occurring when cells actively divide. However, in cancer cells genetic aberrations cause the gene to be expressed in an uncontrolled fashion. C-Myc antibody studies have revealed abnormal expression of the protein in 90% of gynecological cancers and 80% of breast tumors. Overexpression has also been reported in 70% of colon cancers and 50% of hepatic...

Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differentiation, morphology, migration, cell survival and the maintenance of tissue phenotypes. Laminin antibody studies have played a key role in research into angiogenesis and tumor development.

The ECM plays an essential role in angiogenesis, providing support for the developing blood vessel and releasing pro- and anti-angiogenic factors which regulate stability and cell survival. During angiogenesis, the ECM basal membrane is degraded, enabling migration of endothelial cells. Although angiogenesis is necessary for wound healing, it is also a feature of metastasis and tumor formation.

One of six actin isoforms so far identified, beta actin is a highly conserved cytoskeletal protein involved in cell structure, motility, and cohesion. We at Novus Biologicals have many different beta-actin products in our catalog, used in areas such as cytoskeleton and signal transduction research. They are also used as loading controls in protein assays. Beta-actin is known as a “housekeeping” protein, i.e. it is expressed constantly, and at high levels, in all the cell types used in protein research. It is therefore a useful loading control in, for example, Western blot analysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. So long as the molecular weight of the test protein is...

The ABCA1 (ATP-binding cassette transporter-A1) gene encodes a transmembrane protein, which plays a major role in phospholipid homeostasis by regulating cholesterol efflux from the cell. ABCA1 antibody studies have shown ABCA1 expression is up/down regulated by the transcription factors LXR and SREBP2 respectively. Now, new evidence points to microRNA (mRNA) being integral to SREBP2 down-regulation of ABCA1. We at Novus Biologicals have a large number of LXR, SREBP2 and ABCA1 antibody products in our antibody catalog.

ABCA1 is expressed on the Golgi body and plasma membrane. Expression is regulated by transcription factors in response to alterations in the cholesterol flux. Its role is to mediate ...

Ischemic cardiovascular disease is the leading cause of death for both men and women in the US and most other industrialized countries. Most commonly caused by atherosclerosis of the coronary arteries, ischemic cardiovascular disease is characterized by a reduced blood supply to the heart, leading to low oxygen, glucose, and pH levels.

Therapeutic angiogenesis using bone marrow cells has become a promising new field of treatment for ischemic cardiovascular disease (R Laham, et al.). However, maintaining the bone marrow-derived angiogenic cells (BMDACs) under an ischemic microenvironment remains a major obstacle for this treatment.

...

Novus Biologicals is one of the leading antibody suppliers of high affinity human epitope tag reagents, such as the HA tag antibody. Widely used in Western blot, ELISA and Immunohistochemistry assays, they offer a useful and reliable method for detecting, analyzing and purifying proteins from a wide range of species.

Short, immunoreactive epitope tags are highly useful reagents. They are small enough to avoid interfering with the protein under test, yet have a high degree of immunoreactivity, due to the fact they are generally derived from viral genes. The HA tag, for example, is derived from amino acids 98-106 of the human influenza haemagglutinin (HA) protein, an antigenic glycoprotein found on the surface of the human influenza virus.

Developed around 20 years ago, the HA tag can be expressed from a wide range of engineered recombinant proteins, and is widely used as a general...

MMP2 antibodies have proven to be important tools for cancer research and extracellular matrix studies. Novus Biologicals offers an excellent MMP2 antibody (Catalog Number NB200-114) for Western blot, immunoprecipitation, ELISA, and immunohistochemical/immunofluorescence staining in human, mouse and rat samples. Originally developed in 2005, this MMP-2 clone (8B4) has been thoroughly characterized and cited in a growing number of published journal articles. Clone MMP2/8B4 is now considered a one of the best available commercial antibodies for MMP2 immunostaining.

MMP2 is a Matrix Metalloproteinase (MMP) family member involved in extracellular matrix degradation under both normal and diseased physiological processes. Specifically, MMP-2 initiates the degradation of collagens type IV, V, VII, and X and gelatin type I. Additionally, MMP 2 has shown...

The ATP Binding Cassette Transporter (ABCA1) gene encodes the cholesterol regulatory efflux protein, which plays a key role in lipid metabolism. ABCA1 antibody products are an important part of any antibody catalog covering atherosclerosis disease research.

Atherosclerosis (narrowing of the arteries) is characterized by a build-up of fatty plaques, containing cholesterol, causing arteries to narrow. These plaques can then become ulcerated, causing blood clots to form. If this occurs in the carotid arteries the clots can travel to the brain, causing a stroke. In the general population, low plasma HDLC levels are well documented to increase the risk of coronary heart disease.

ABCA1 antibody studies in animal models have shown ABCA1 gene mutations increase the risk of atherosclerosis. Researchers also discovered that...

Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recombination at double-strand breaks. In 2010, German Rad51 antibody studies showed Rad51 levels were significantly raised in canine metastatic tumors. In general there was significant correlation between canine and human oncogenes, suggesting a possible animal model for human breast cancer.

Double-strand breaks (DSBs) are extremely common, and are caused by a number of environmental factors including ionizing radiation,...

We at Novus Biologicals have a large HIF-1 (Hypoxia Inducible Factor-1) antibody catalog, with twenty HIF-1 beta antibody products specifically targeted to the beta subunit of HIF-1, a heterodimer which is known to contribute to many human diseases arising from changes in oxygen homeostasis.

HIF-1 is a transcription factor and key mediator of homeostasis triggered by hypoxia. It drives the transcription of more than 60 genes concerned with anaerobic metabolism, cell survival, cytokine production, the immune reaction, vascularisation and all areas of tissue homeostasis. It is also a key hypoxic regulator of embryonic vascular development.

...

The notch proteins are single-pass membrane receptors controlling a highly conserved signalling pathway. In vertebrates, the notch family comprises four transmembrane receptors encoded by four individual genes (NOTCH1 - 4). We at Novus Biologicals are leading NOTCH antibody suppliers, with an extensive range of NOTCH antibody products.

In 1999, the notch signalling cascade was shown to play multiple roles in cell fate decisions, controlling the cellular response to intrinsic or extrinsic signals associated with developmental programming. Notch signalling is an important initiator of cellular differentiation and proliferation, as well as apoptosis, and a basic developmental tool for morphogenesis and organ formation.

...