Neuroscience Research using Three Neurofilament Antibodies

Fri, 04/29/2011 - 07:50

Neurofilaments (NFs) are intermediate filaments found almost exclusively in neuronal cells, and play an essential role supporting the cytoskeleton. In vertebrates they are composed of three intertwining polypeptide subunits of varying length and molecular weight – the 68kDa light, 160kDa medium and 200kDa heavy Neurofilament chains. We at Novus Biologicals have an extensive range of top quality neurofilament antibody products in our antibody database.

The three NF chains share similar structures and base sequences, but vary in length and sequence at both the N and C-termini. In NF-H and NF-M subunits, the C-termini form extensions which link neurofilaments both to each other and to other cytoplasmic proteins.

Immunocytochemistry: 68kDa Neurofilament Light Antibody

Neurofilament antibody research advanced a long way with the advent of SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), which enables proteins to be separated and accurately measured according to their electrophoretic mobility. NF-L, M and H subunits are 68-70kDa, 145-160 kDa and 200-220kDa in size, respectively, with protein sizes varying according to species.

Neurofilament antibody products are widely used in neuropathology immunostaining assays, as overexpression of neurofilaments is a feature of many diseases. They are routinely used in NF cell marker studies.

In 2002, Duplan, et al. used 160kDa neurofilament antibody assays to study anti-tumor FGF (fibroblast growth factor) activity relative to FGF receptor expression in medulloblastoma tumor variants. More recently the 160kDa neurofilament antibody has been used in a study linking human TARDBP expression in mouse motor neurons to growth deformities and early death. The NF products on our antibody database are suitable for use in a wide range of assays including western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation.

Novus Biologicals offers many Neurofilament reagents for your research needs including:


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