Antibody suppliers

E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epithelial cell glycoprotein whose extracellular domain interacts with that of other E-cadherin molecules on adjacent cells to establish cell-cell adhesion. This adhesion is Ca^2+-dependent and a variety of interactions have been identified.

Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back and forth diffusion of low molecular weight molecules. As such, they regulate quite an extensive range of key cellular processes.

Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding of iron to transferrin can be related to pH levels, among other things, with a high binding affinity for a more acidic pH and decreasing levels as the blood pH becomes more basic. This binding affinity is the basis for the transport mechanism of Transferrin.

Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small protein growth factors that play a role in survival and differentiation of distinct cell populations.

Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA.

Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1), also known as known as CD31 (cluster of differentiation 31), is an Ig supergene family cell adhesion and signaling membrane receptor. CD31/PECAM1 is critical for cell-cell interactions and is expressed on a variety of hematopoetic cells (platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets).

Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital cellular processes such as chromatin remodeling, DNA repair, sister-chromatid cohesion and cell cycle control as well (1).

Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expressed in abundance in exponentially growing cells and it regulates various aspects of DNA and RNA metabolism, chromatin structure, rDNA transcription, rRNA maturation, cytokinesis, nucleogenesis, cell proliferation and growth (1).

Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them by immnoflorescence, immunoprecipitation and by Western blotting assays.

The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impacts most major cellular functions.