Using CD31/PECAM1 Antibodies as Reliable Endothelial Cell Junction Markers

Tue, 06/05/2012 - 09:11

Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1), also known as known as CD31 (cluster of differentiation 31), is an Ig supergene family cell adhesion and signaling membrane receptor. CD31/PECAM1 is critical for cell-cell interactions and is expressed on a variety of hematopoetic cells (platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets). Because this adhesion molecule is expressed at high levels specifically localized to endothelial cell junctions, CD31/PECAM1 antibodies can be used as endothelial markers1.

Immunohistochemistry-Frozen: CD31/PECAM1 Antibody

In the field of angiogenesis, the classic family of integrins has been long studied but experiments with CD31/PECAM1 antibodies demonstrate that other adhesion molecules like cadherin and CD31/PECAM1 also have an important function and can inhibit blood vessel formation in different animal models of growth factor/chemokine-induced angiogenesis 2,3.  The precise mechanism remains to be elucidated but it is thought that CD31/PECAM1 may initially stabilize critical endothelial cell-cell contact as well as upregulate integrin expression. CD31/PECAM1 antibodies have allowed detailed study of this target molecule and its structure-function has been well-characterized, but its role in inflammation is not as clear. It plays dual and opposing roles – having both pro-inflammatory (aiding leukocyte migration and transducing fluid shear stress signals) and anti-inflammatory (suppressing cytokine production and maintaining vascular barrier integrity) functions. Further work is required to understand and define the underlying mechanisms to allow the use of a CD31/PECAM1 antibody in diagnosing and enabling treatment of both acute and chronic inflammatory disorders 4.

  1. PMID: 8892086
  2. PMID: 9120301
  3. PMID: 1857836
  4. PMID: 20541560


Novus Biologicals offers CD31/PECAM1 reagents for your research needs including:



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