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Smoothened Antibody

 (7 Publications)
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Summary
Product Discontinued
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    • Catalog Number
      NBP2-24543
    • Availability
      Product Discontinued

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Smoothened Antibody Summary

Immunogen
A portion of amino acids 190-240 of human SMO was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMO
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 0.5-2 ug/ml
Publications
Read Publications using
NBP2-24543 in the following applications:

  • IB
    1 publication
  • ICC/IF
    2 publications
  • IHC-P
    3 publications
  • WB
    1 publication

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human ( isoforms CRA_a, CRA_b, CRA_c), rhesus monkey and chimpanzee, 94% homologous in horse, cow and dog, 88% homologous in rat, mouse, chicken, opossum, zebrafish and Xenopus and 83% homologous in pufferfish.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smoothened Antibody

  • bnb
  • CRJS
  • FZD11
  • Gx
  • Protein Gx
  • SMO
  • SMOH
  • SMOHseven transmembrane helix receptor
  • smoothened (Drosophila) homolog
  • smoothened homolog (Drosophila)
  • smoothened homolog
  • Smoothened

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smoothened Antibody (NBP2-24543)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IB, ICC/IF, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(2)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-24543 Applications Species
Belgacemi R, Luczka E, Ancel J Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine 2019-12-23 [PMID: 31877414] (IB, Human) IB Human
Levy D, de Melo TC, Oliveira BA, et al 7-Ketocholesterol and cholestane-triol increase expression of SMO and LXR alpha signaling pathways in a human breast cancer cell line. Biochem Biophys Rep. 2018-12-31 [PMID: 31463370] (ICC/IF, Human)

Details:
A human mammary gland/breast cell line MDA-MB-231 was used in this study. 		ICC/IF Human
Paz JL, Levy D, Oliveira BA, de Melo TC et al. 7-Ketocholesterol Promotes Oxiapoptophagy in Bone Marrow Mesenchymal Stem Cell from Patients with Acute Myeloid Leukemia Cells 2019-05-21 [PMID: 31117185] (ICC/IF, Human) ICC/IF Human
Zhang Y, He J, Zhang F et al. SMO expression level correlates with overall survival in patients with malignant pleural mesothelioma. J Exp Clin Cancer Res. 2013-02-05 [PMID: 23379358]
Bolanos AL, Milla CM, Lira JC et al. Role of Sonic Hedgehog in idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2012-12-01 [PMID: 23023967] (IHC-P, Human)

Details:
Smoothened antibody (IMG 5904A) IHC-P ; Human Lung. (See Fig 3) 		IHC-P Human
Tao Y, Mao J, Zhang Q, Li L. Overexpression of Hedgehog signaling molecules and its involvement in triple-negative breast cancer. Oncol Lett. 2011-09-01 [PMID: 22866163] (WB, IHC-P, Human)

Details:
Smoothened antibody (IMG 5904A) WB and IHC-P ; Human breast. (See Figs 1,2). 		WB, IHC-P Human
Walter K, Omura N, Hong SM et al. Overexpression of smoothened activates the sonic hedgehog signaling pathway in pancreatic cancer-associated fibroblasts. Clin Cancer Res. 2010-03-15 [PMID: 20215540] (IHC-P, Human)

Details:
Smoothened antibody (IMG 5904A) IHC-P (human). 		IHC-P Human

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Smoothened Antibody (NBP2-24543). (Showing 1 - 1 of 1 FAQs).

  1. Please let us know the best substitute product for Smoothened Antibody (NBP2-24543 ) which got discontinued?
    • Based on the species and applications that Smoothened antibody (NBP2-24543 ) was suggested for, the possible alternative would be NLS2666. The latter is a rabbit polyclonal that is good for WB and IHC-P applications in Human, mouse, and Primate species. 

Secondary Antibodies

 

Isotype Controls

Additional Smoothened Products

Research Areas for Smoothened Antibody (NBP2-24543)

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Blogs on Smoothened.

Check out the latest blog posts on Smoothened.
The role of Smoothened in pulmonary pathologies
The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche...  Read full blog post.
Pancreatic Cancer Research Targets Hedgehog Signaling Pathway
The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact...  Read full blog post.

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Bioinformatics

Gene Symbol SMO