| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60011SS | |||
| Azide and BSA Free | NBP2-81012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-165SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-42821SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51843SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97311SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43262-0.5mg | |||
| BSA Free | NBP1-43262-25ug | |||
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0007.jpg "Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-Paraffin-NB110-60011-img0006.jpg)
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0005.jpg)
![Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-NB110-60011-img0003.jpg)
![Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Simple-Western-NB110-60011-img0004.jpg)
![Western Blot: WT1 Antibody (6F-H2) - BSA Free [NB110-60011] - Hypomethylation of Intron 1 CpG island results in WT1 expression. (C) Cell lysates from K562, U937, & HL60 cells treated with CoCl2, 5-azacytidine, or untreated controls analyzed by WB using an antibody against WT1. Histone H3 used as a loading control. Size of molecular weight markers is in kDa. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-60011_mouse-monoclonal-wt1-antibody-6f-h2-310202415304296.jpg)
![Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Western-Blot-NB100-119-img0003.jpg "Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Immunocytochemistry-Immunofluorescence-NB100-119-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Immunohistochemistry-Paraffin-NB100-119-img0001.jpg)
![Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0007.jpg "Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Immunohistochemistry-Frozen-NBP1-28566-img0002.jpg)
![Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a verified end user.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0005.jpg)
![Western Blot: Bax Antibody (6A7) [NBP1-28566] - Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probled with Mouse Anti-Bax UNLB.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0006.jpg)
![Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.](http://images.novusbio.com/fullsize/XRCC3-Antibody-Western-Blot-NB100-165-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.](http://images.novusbio.com/fullsize/XRCC3-Antibody-Western-Blot-NB100-165-img0004.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0008.jpg "Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).")
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Two cores from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray demonstrate the variable expression of Bid protein. The sections were stained with Bid antibody at 1:2000 with hematoxylin-eosin counterstain. Section A shows very weak staining while section B stains much more strongly.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0009.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of BID in NRK whole cell lysate using anti-BID antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0007.jpg)
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0010.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0009.jpg "Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-471-img0004.jpg)
![Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0005.jpg)
![Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/WRN-Antibody-Immunoprecipitation-NB100-471-img0008.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42821-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42821-img0006.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate from primary rat hepatocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-672023145728..jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate from mice hepatocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-672023145955..jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate of HepG2 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-67202315152..jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal p62/SQSTM1 Antibody [NBP1-42821] - Mouse hepatocytes stained with p62/SQSTM1 Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-immunocytochemistry-immunofluorescence-25112024113332..png)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0012.jpg "Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Simple-Western-NBP1-51843-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0008.jpg)
![Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-51843-img0011.jpg)
![Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-NBP1-51843-img0003.jpg)
![Simple Western: Aurora A Antibody - BSA Free [NBP1-51843] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51843_rabbit-polyclonal-aurora-a-antibody-308202410445515.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56101-img0002.jpg "Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.")
![Western Blot: TRPM8 Antibody [NBP1-97311] - The results of co-immunoprecipitation (CoIP) confirmed interaction between NFkB and TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 and NFkB. Data are shown as the mean +/- S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Knockdown-Validated-NBP1-97311-img0007.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: TRPM8 Antibody [NBP1-97311] - The results of co-immunoprecipitation (CoIP) confirmed interaction between NFkB and TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 and NFkB. Data are shown as the mean +/- S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - Co-localization of TRPM8 and TNFa in the cytoplasm (cold condition and 500 nM menthol). Data are shown as the mean +/- S.D. from three experiments. **P < 0.01.Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97311-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TRPM8 Antibody [NBP1-97311] - Brain cooling means putting the anesthetized mouse brain on the artificial ice. Normal means the room temperature (25C). Data are shown as the mean +/- S.D. from five mice in each group. #P < 0.05; ##P < 0.01, v.s. the normal control. *P < 0.05; v.s. the CIR. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP1-97311-img0006.jpg)
![Immunohistochemistry: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunohistochemistry-NBP1-97311-img0005.jpg)
![Western Blot: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in PC12 cell lysate.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP1-97311-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - TRPM8 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97311-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Confocal imaging of TRPM8, NF kappa B & TNF alpha expression in PC12 cells.(A) The expression of TRPM8, NF kappa B & TNF alpha in wild type cells & Trpm8 knockdown cells at 37 °C & 4 °C. KD signifies Trpm8 knockdown cells. (B) In wild type cells, TRPM8 was upregulated & NF kappa B & TNF alpha were downregulated under cold conditions. (C) In KD cells, TRPM8 showed weak expression & NF kappa B & TNF alpha expression levels were increased. (D) Co-localization of TRPM8 & TNF alpha in the cytoplasm (cold condition & 500 nM menthol). Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-31020241536377.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappa B & TNF alpha in PC12 cells with Trpm8 knockdown under cold conditions (4 °C).(A) Construction of a Trpm8 (Dylight 649) knockdown stable cell line. WT represents wild type cells. KD signifies the Trpm8 knockdown cells. (B,C) Protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . NS: no significance. Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415291969.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Alteration of core temperature & the expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha in mouse brains under cold conditions.(A) Core body temperature under cold conditions (4 °C). (B) The mRNA expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . (C,D) The protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . Data are shown as the mean ± S.D. from 12 mice in each group. ##v.s. the control (zero hour), P < 0.01; #P < 0.05. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415392570.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Co-localization of TRPM8 & NF kappa B in PC12 cells under cold conditions.(A) Immunofluorescence assay image of TRPM8 & NF kappa B in the cytoplasm. WT represents wild type cells. KD represents Trpm8 knockdown cells. (B) The results of co-immunoprecipitation (CoIP) of endogenous TRPM8 & NF kappa B using NF kappa B antibodies. Western blot analysis was carried out to detect TRPM8 & NF kappa B. (C) Reverse CoIP confirmed interaction between NF kappa B & TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 & NF kappa B. Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-31020241539721.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappa B & TNF alpha in PC12 cells under cold conditions.(A) Intracellular Ca2+ in the cells under cold conditions (4 °C). The Ca2+ concentration in the cytoplasm at 4 °C is higher than that at 37 °C. (B) The mRNA expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . (C,D) The protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415304224.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0020.jpg "Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Mouse (B7-H1) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0019.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0021.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0011.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0010.jpg)