p73 Antibody (5B429) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC-Fr, IHC-P, IP
Clone
5B429
Clonality
Monoclonal
Host
Mouse

p73 Antibody (5B429) [DyLight 650] Summary

Immunogen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Specificity
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 650
Format
DyLight 650 (A=652, E=672)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for p73 Antibody (5B429) [DyLight 650]

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for p73 Antibody (NBP2-24737C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-24737APC
Biotin Labeled NBP2-24737B
DyLight 350 Labeled NBP2-24737UV
DyLight 405 Labeled NBP2-24737V
DyLight 405LS Labeled NBP2-24737V3
DyLight 488 Labeled NBP2-24737G
DyLight 550 Labeled NBP2-24737R
DyLight 680 Labeled NBP2-24737FR
DyLight 755 Labeled NBP2-24737IR
HRP Labeled NBP2-24737H
PE Labeled NBP2-24737PE
PerCP Labeled NBP2-24737PCP

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol TP73

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