| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64988SS | |||
| Azide and BSA Free | NBP2-80647 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24716SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-37258SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85578-25ul | |||
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0008.jpg "Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cell-Surface-NB100-64988-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Immunocytochemistry-Immunofluorescence-NB100-64988-img0007.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunohistochemistry-Frozen-NB100-64988-img0001.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.](http://images.novusbio.com/fullsize/MTSS1-Antibody-Western-Blot-NBP2-24716-img0005.jpg "Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: MTSS1 Antibody [NBP2-24716] - Analysis of human colon using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/MTSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-24716-img0004.jpg)
![Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Western-Blot-NB110-37258-img0005.jpg "Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SLIRP (NB110-37258) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37258-img0009.jpg)
![Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunohistochemistry-NB110-37258-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37258-img0006.jpg)
![Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Simple-Western-NB110-37258-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0011.jpg "Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0005.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0010.jpg)
![Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP1-33202-img0006.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody (NBP1-33202) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33202_rabbit-polyclonal-staufen-antibody-immunocytochemistry-immunofluorescence-26112025185041.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 mRNA and protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative and un-transfected HO-8910PM cells were determined by RT-PCR and Western blot analysis, respectively. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Knockdown-Validated-H00008826-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 mRNA and protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative and un-transfected HO-8910PM cells were determined by RT-PCR and Western blot analysis, respectively. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - Western blot analysis for IQGAP1 in whole-cell lysates from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Western-Blot-H00008826-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00008826-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunohistochemistry-Paraffin-H00008826-M01-img0008.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 monoclonal antibody (M01), clone 2C5 Analysis of IQGAP1 expression in C32.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Western-Blot-H00008826-M01-img0010.jpg)
![Sandwich ELISA: IQGAP1 Antibody (2C5) [H00008826-M01] - Detection limit for recombinant GST tagged IQGAP1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Sandwich-ELISA-H00008826-M01-img0009.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - indicates that IQGAP1 mRNA & protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative & un-transfected HO-8910PM cells were determined by RT-PCR & Western blot analysis, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415345311.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - shows that IQGAP1 expression & cell invasive phenotype in ovarian cancer cell lines. (a) RT-PCR analysis for IQGAP1 using total RNA form HO-8910, SK-OV-3 & HO-8910PM cells. (b) Western blot analysis for IQGAP1 in whole-cell lysates from the indicated cell lines. (c) High IQGAP1 expression associated with enhanced invasive potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Matrigel-coated invasion chamber & the number of invading cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. (d) High IQGAP1 expression correlated with enhanced migratory potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Boyden chamber & the number of migrating cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415483611.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415482215.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-31020241548516.jpg)
![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - Parkin knockout improves the decline of mitochondrial dynamics proteins in SOD1-G93A spinal cord, but mitochondrial protein ubiquitination profiles and March5 and Mul1 levels are unaffected A, B Western blots (A) and quantification (B) of Miro1 in spinal cord homogenates at disease end stage. Beta-actin is used as loading reference. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0005.jpg "Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - Parkin knockout improves the decline of mitochondrial dynamics proteins in SOD1-G93A spinal cord, but mitochondrial protein ubiquitination profiles and March5 and Mul1 levels are unaffected A, B Western blots (A) and quantification (B) of Miro1 in spinal cord homogenates at disease end stage. Beta-actin is used as loading reference. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888) licensed under a CC-BY license.")
![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0004.jpg)
![ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-ELISA-H00055288-M01-img0003.jpg)
![Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12. Analysis of CFLAR expression in K-562.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Western-Blot-H00008837-M01-img0012.jpg "Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12. Analysis of CFLAR expression in K-562.")
![Immunocytochemistry/Immunofluorescence: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Immunocytochemistry-Immunofluorescence-H00008837-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Immunohistochemistry-Paraffin-H00008837-M01-img0009.jpg)
![Western Blot: FLIP Antibody (2F12) [H00008837-M01] - Western blot of FLIP provided courtesty of verified customer.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Western-Blot-H00008837-M01-img0005.jpg)
![Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12 Analysis of CFLAR expression in IMR-32.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Western-Blot-H00008837-M01-img0011.jpg)
![Western Blot: FLIP Antibody (2F12) [H00008837-M01] - Analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M01), clone 2F12.Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Western-Blot-H00008837-M01-img0013.jpg)
![Sandwich ELISA: FLIP Antibody (2F12) [H00008837-M01] - Detection limit for recombinant GST tagged CFLAR is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FLIP-Antibody-2F12-Sandwich-ELISA-H00008837-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining in human liver and testis tissues . Corresponding MAOB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining in human liver and testis tissues . Corresponding MAOB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-87493-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in human liver tissue.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-87493-img0005.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0008.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0009.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0010.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining in human liver and pancreas tissues using anti-ASGR2 antibody. Corresponding ASGR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining in human liver and pancreas tissues using anti-ASGR2 antibody. Corresponding ASGR2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human colon, liver, lymph node and pancreas using Anti-ASGR2 antibody NBP1-85578 (A) shows similar protein distribution across tissues to independent antibody NBP1-85579 (B).](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0014.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human pancreas using Anti-ASGR2 antibody NBP1-85578.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0018.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0009.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0010.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0015.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human colon.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0016.jpg)
![Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Staining of human liver using Anti-ASGR2 antibody NBP1-85578.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85578-img0017.jpg)
![Simple Western: Asialoglycoprotein Receptor 2 Antibody [NBP1-85578] - Simple Western lane view shows a specific band for ASGR2 in 0.2 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Asialoglycoprotein-Receptor-2-Antibody-Simple-Western-NBP1-85578-img0006.jpg)
