Signal Transduction reagents: Novus Biologicals

NBP1-80858

RAB5C Antibody - BSA Free

(2 Reviews)

(7 Publications)

0.1 ml

NBP1-80858

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-80858-25ul

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NBP1-85814

Vimentin Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-85814

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85814-25ul

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NBP2-57308

MCT8/SLC16A2 Antibody - BSA Free

(6 Publications)

100 ul

NBP2-57308

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cerebral cortex shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human liver shows moderate to strong membranous positivity in hepatocytes.

Expression of MCT8 and OATP1C1 in the cerebral barriers of the human and macaque motor cortex. Representative brightfield (A,E,I,M) and fluorescence confocal microscope (B–D,F–H,J–L,N–T) photomicrographs showing immunostaining for MCT8 (A–H,Q–T) and OATP1C1 (I–P) in blood vessels of human and macaque motor cortex. (A,E) show strong MCT8 staining in the endothelial layer of small vessels and capillaries (white arrowheads) in humans and macaques, respectively. (B–D,F–H) show colocalization of MCT8 (green) with the endothelial markers UEA-I (red) and ENG (red). (I,M) show weak OATP1C1 staining in small vessels (white arrowheads) and venules (black arrowheads). The red arrowhead in M points to a glial cell positive for OATP1C1 with processes surrounding the capillary. (J–L,N–P) show colocalization of OATP1C1 (green) with UEA-I (red) and endoglin (red) in a few blood vessels of the human and macaque motor cortex, respectively. White arrowheads point to vessels. In both human and macaque brain tissues, MCT8-immunopositive capillaries are much more abundant than OATP1C1-immunopositive capillaries, which can only be seen occasionally. Moreover, (Q–T) show MCT8-expressing pericytes (white arrowheads) in the human motor cortex that in addition to express MCT8 (green), also show the pericyte biomarker PDGFR-beta (pink), but not the endothelial marker UEA-I (red). Counterstaining with DAPI (blue) shows nuclei of all cells. ENG: endoglin, UEA-I: Ulex Europaeus Agglutinin-I. PDGFR-beta : platelet-derived growth factor receptor beta. Scale bar = 100 μm (A,E,I,M), 25 μm (B–D), 50 μm (J–L), 120 μm (F–H,N–P), and 12 μm (Q–T). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 in pyramidal neurons of the human and macaque motor cortex. Representative brightfield photomicrographs show immunostaining for MCT8 in layers III (A), V (B) and VI (C) of the human motor cortex, and layers II (G), III (H), and V (I) of the macaque motor cortex. White arrowheads point to pyramidal neurons with immunopositive signal in the soma, apical and basal dendrites. Black arrowheads point to smaller cells with the morphology of interneurons. (D–F) (human) and (J–L) (macaque) show confocal microscope images from double-stained sections for MCT8 (green) and the pyramidal neuron markers SMI-32 (red) for 200 kDa neurofilament protein or neurogranin/RC3 (red), respectively. White arrowheads point to pyramidal neurons. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans, the MCT8 signal is located mainly in the pyramidal cell membrane, while in macaques it is located in the membrane and the cytoplasm. Scale bar = 50 μm (A), 120 μm (B,I), 25 μm (C–F), 60 μm (G), 62.5 μm (H), and 43.5 μm (J–L). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 and OATP1C1 in human and macaque motor cortex astrocytes. Representative brightfield (A–E,I) photomicrographs of sections immunostained for MCT8 (A,C) and OATP1C1 (B,D,E,I) in cortical layer I (A–D) and subjacent white matter (E,I). Confocal microscope images of human (F–H) and macaque (J–O) sections double-labeled for OATP1C1 (green) and GFAP (red). Counterstaining with DAPI (blue) shows nuclei of all cells. Note that there is full colocalization of both markers. White and red arrowheads point to astrocytes. Blue arrowheads point to a blood vessel. GFAP: glial fibrillary acidic protein. Scale bar = 50 μm (A–D,J–L), 110 μm (E,I), 25 μm (F–H), and 78 μm (M–O). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57308-25ul

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NBP2-02577

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

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NBP1-76511

Klotho Antibody - BSA Free

(5 Publications)

0.1 mg

NBP1-76511

Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Biological Strategies Validation. </b>Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.

Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P < .001, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. H, RT‐PCR was performed to measure the expression of miRNA‐34a. **P < .01, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. I, FFA was performed to measure the leakage of CNV. J, The leakage of CNV was analysed. K, ICGA was performed to measure the area of CNV. L, The area of CNV was analysed. **P < .01, ***P < .001, compared with the CNV 7‐d group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - microrna‐34a inhibits the expression of Klotho. ARPE‐19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin‐3a, hypoxia + AAV‐p53 mutant infection & hypoxia + miRNA‐34a inhibitor. A, Western blot assay of Klotho in ARPE‐19 cells was performed. B, The relative protein level of Klotho compared with the GAPDH level was analysed. ***P < .001, hypoxia group vs normal group. ###P < .001, ##P < .01, compared with the hypoxia group. %%P < .01, hypoxia + digoxin + nutlin‐3a group vs hypoxia + digoxin group. C, Schematic of the Klotho 3' UTR with the WT or mutated putative‐binding site of miRNA‐34a inserted into a luciferase (Luc) reporter. D, qRT‐PCR analysis of Klotho mRNA expression in HEK293T cells transfected with miRNA‐34a mimic for 24 h. **P < .01, miRNA‐34a mimic group vs control group. E, Western blot of Klotho in HEK293T cells transfected with a miRNA‐34a mimic for 24 h was performed. F, Immunoblotted Klotho was quantified & normalized to GAPDH. ***P < .001, miRNA‐34a mimic group vs control group. G Relative luciferase activity was detected in HEK293T cells co‐transfected with plasmids containing firefly luciferase & wild‐type (WT) or mutant (Mut) Klotho 3′‐UTR & miRNA‐34a mimic for 24 h. The luciferase activity values were normalized to Renilla reniformis luciferase (TK‐RL) activity. **P < .01, Luc‐Klotho 3′ UTR WT group vs vector group. Statistically non‐significant (NS), Luc‐Klotho 3′ UTR mutant group vs vector group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - High phytate-induced renal phosphate wasting is independent of FGF23.(A) The renal expression of downregulated genes associated with Ca2+ & phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). (B) Results of immunoblot analyses & protein levels of renal aKlotho, NHERF1, & NaPi-2a after 2 weeks for rats fed control, HP- LCa2+ , & HP-HCa2+ diets. (C) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). (D, E) Time course analysis of serum levels of intact FGF23 (D) & C-terminal FGF23 (E) in rats fed control, HP-LCa2+ , & HP-HCa2+ diets. (F) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa2+ , & HP-HCa2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32271147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

WD feeding induced changes in FGF-23 and Klotho expression are restored by DPP-4 inhibition. a FGF-23 staining; b Endothelial FGF-23; c Adventitia FGF-23; d Endothelial klotho staining; e Endothelial klotho staining f Adventitia klotho staining; Average gray intensities in the different cohorts. Values are mean +/- SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control and WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0 0.05 CDC vs WDC; †p < 0.05 WDC vs WDL; #p < 0.05 CDC vs CDL. Scale bars represent 50 mμ Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27391040), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76511AF350
Alexa Fluor 405	NBP1-76511AF405
Alexa Fluor 488	NBP1-76511AF488
Alexa Fluor 532	NBP1-76511AF532
Alexa Fluor 594	NBP1-76511AF594
Alexa Fluor 647	NBP1-76511AF647
Alexa Fluor 700	NBP1-76511AF700
Alexa Fluor 750	NBP1-76511AF750
Allophycocyanin	NBP1-76511APC
Biotin	NBP1-76511B
CoraFluor 1	NBP1-76511CL1
DyLight 350	NBP1-76511UV
DyLight 405	NBP1-76511V
DyLight 488	NBP1-76511G
DyLight 550	NBP1-76511R
DyLight 594	NBP1-76511DL594
DyLight 650	NBP1-76511C
DyLight 680	NBP1-76511FR
DyLight 755	NBP1-76511IR
FITC	NBP1-76511F
HRP	NBP1-76511H
Janelia Fluor 525	NBP1-76511JF525
Janelia Fluor 549	NBP1-76511JF549
Janelia Fluor 646	NBP1-76511JF646
Janelia Fluor 669	NBP1-76511JF669
PE	NBP1-76511PE
PerCP	NBP1-76511PCP
mFluor Violet 450 SE	NBP1-76511MFV450
mFluor Violet 500 SE	NBP1-76511MFV500
mFluor Violet 610 SE	NBP1-76511MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76511-0.025mg

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NBP1-52849

Cystathionase Antibody - BSA Free

(3 Reviews)

(8 Publications)

100 ul

NBP1-52849

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-67639

NLRP3/NALP3 Antibody (SC06-23)

(4 Publications)

100 ul

NBP2-67639

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-67433

STAT3 [p Ser727] Antibody (SY24-09)

(1 Review)

100 ul

NBP2-67433

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate

Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Human U251 glioma cell line, whole cell lysates.Cells were treated with SP600125, DB07268, JNK-IN-8 and SB203580. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-66888

HMG-CoA Reductase/HMGCR Antibody (JF0981)

(1 Review)

(7 Publications)

100 ul

NBP2-66888

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-45283

SNX27 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-45283

Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.

Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SXN27 antibody in A. 293T + tagged SNX27 protein and B. 293T empty vector.

Simple Western: SNX27 Antibody [NBP1-45283] - Simple Western lane view shows a specific band for SNX27 in 0.5 mg/ml of Human Cerebellum (left) and Mouse Cerebellum (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Bv, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-45283AF350
Alexa Fluor 405	NBP1-45283AF405
Alexa Fluor 488	NBP1-45283AF488
Alexa Fluor 532	NBP1-45283AF532
Alexa Fluor 594	NBP1-45283AF594
Alexa Fluor 647	NBP1-45283AF647
Alexa Fluor 700	NBP1-45283AF700
Alexa Fluor 750	NBP1-45283AF750
Allophycocyanin	NBP1-45283APC
Biotin	NBP1-45283B
CoraFluor 1	NBP1-45283CL1
DyLight 350	NBP1-45283UV
DyLight 405	NBP1-45283V
DyLight 488	NBP1-45283G
DyLight 550	NBP1-45283R
DyLight 594	NBP1-45283DL594
DyLight 650	NBP1-45283C
DyLight 680	NBP1-45283FR
DyLight 755	NBP1-45283IR
FITC	NBP1-45283F
HRP	NBP1-45283H
Janelia Fluor 525	NBP1-45283JF525
Janelia Fluor 549	NBP1-45283JF549
Janelia Fluor 646	NBP1-45283JF646
Janelia Fluor 669	NBP1-45283JF669
PE	NBP1-45283PE
PerCP	NBP1-45283PCP
mFluor Violet 450 SE	NBP1-45283MFV450
mFluor Violet 500 SE	NBP1-45283MFV500
mFluor Violet 610 SE	NBP1-45283MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-45283SS

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NB110-37258

SLIRP Antibody - BSA Free

(1 Publication)

0.1 ml

NB110-37258

Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

MUP1 is regulated by high-fat diet (HFD) and exercise training (ET). Heatmaps (A) of proteins altered (q < 0.05 and fold-change +/−50%) by ET on Chow (top), HFD (middle), or ET on HFD (bottom heatmap) with corresponding proteins from the other groups. Proteins depicted in (B) were either altered in the same direction by ET and ET on HFD, or they were oppositely altered by HFD and ET on Chow or ET on HFD (‘+’ denotes increased, ‘−’ decreased abundance; a diagonal strikethrough means that the protein was not significantly regulated). Quantification and representative images of western blot performed for the indicated proteins (C–G, n = 5–10). The effects of ET and HFD on Mup1 gene expression in muscle (H, n = 7–10), as well as the effect of ET effect on Mup1 gene expression in AMPK KD mice (I, n = 8). Data are means +/− SEM. ***p < 0.001 main effect of diet (HFD vs Chow). ###p < 0.001 and ##p < 0.01 main effects for activity (ET vs sedentary (SED)). ¤¤p < 0.01 difference between indicated groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30013070), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-37258AF350
Alexa Fluor 405	NB110-37258AF405
Alexa Fluor 488	NB110-37258AF488
Alexa Fluor 532	NB110-37258AF532
Alexa Fluor 594	NB110-37258AF594
Alexa Fluor 647	NB110-37258AF647
Alexa Fluor 700	NB110-37258AF700
Alexa Fluor 750	NB110-37258AF750
Allophycocyanin	NB110-37258APC
Biotin	NB110-37258B
CoraFluor 1	NB110-37258CL1
DyLight 350	NB110-37258UV
DyLight 405	NB110-37258V
DyLight 488	NB110-37258G
DyLight 550	NB110-37258R
DyLight 594	NB110-37258DL594
DyLight 650	NB110-37258C
DyLight 680	NB110-37258FR
DyLight 755	NB110-37258IR
FITC	NB110-37258F
HRP	NB110-37258H
Janelia Fluor 525	NB110-37258JF525
Janelia Fluor 549	NB110-37258JF549
Janelia Fluor 646	NB110-37258JF646
Janelia Fluor 669	NB110-37258JF669
PE	NB110-37258PE
PerCP	NB110-37258PCP
mFluor Violet 450 SE	NB110-37258MFV450
mFluor Violet 500 SE	NB110-37258MFV500
mFluor Violet 610 SE	NB110-37258MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-37258SS

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NBP1-44048

beta Amyloid [Pyro Glu3] Antibody - BSA Free

(7 Publications)

0.05 mg

NBP1-44048

Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: Beta Amyloid pyro E3 as as green fluorescent signal, Beta Amyloid 3 as yellow signal; and Bottom: Beta Amyloid 3 as red signal with co-incubation of Beta Amyloid pyro E3 peptide.

Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E3 antibody at 2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.

Reactivity:

Hu, Mu, Ca, Dr

Species Glossary

Reactivity: Hu, Mu, Ca, Dr

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Inhibitor Family

Inhibitor Target

Isotype

Kit Type

Lysate Subcellular Fraction

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

RAB5C Antibody - BSA Free

Vimentin Antibody - BSA Free

MCT8/SLC16A2 Antibody - BSA Free

Profilin 1 Antibody (OTI1D5)

Klotho Antibody - BSA Free

Cystathionase Antibody - BSA Free

NLRP3/NALP3 Antibody (SC06-23)

STAT3 [p Ser727] Antibody (SY24-09)

HMG-CoA Reductase/HMGCR Antibody (JF0981)

SNX27 Antibody - BSA Free

SLIRP Antibody - BSA Free

beta Amyloid [Pyro Glu3] Antibody - BSA Free