| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40764SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92687SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22127SS | |||
| Azide and BSA Free | NBP2-80575 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-134SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-2525SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-156SS | |||
| Azide and BSA Free | NBP2-80857 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-103SS | |||
![Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Western-Blot-NB300-731-img0017.jpg "Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0007.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in HeLa Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0006.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0012.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0015.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0016.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody (NB110-40764) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0007.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody (NB110-40764) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40764-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody conjugated to DyLight 550 (NB110-40764R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0008.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle. Representative blots of PLIN3, ATGL, ARF1, ARFRP1 and loading control GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0091675), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40764-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0005.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Knockdown-Validated-NBP1-92687-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0003.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of different cell lysates using mouse mAb to vimentin, NBP1-92687, dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Western-Blot-NBP1-92687-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin, NBP1-92687, dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (2D1) [NBP1-92687] - Human breast cancer tissue stained for Vimentin (white) and countersatined with DAPI (blue). Alexa Fluor 750 version of antibody used (NBP1-92687AF750). Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-Paraffin-NBP1-92687-img0012.jpg)
![Immunohistochemistry: Vimentin Antibody (2D1) [NBP1-92687] - Mouse cortex microslice section (from postnatal day 3) was immunostained with (1:500 dil.). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-NBP1-92687-img0005.jpg)
![Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Simple-Western-NBP1-92687-img0004.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - High glucose (HG) concentrations induced epithelial-to-mesenchymal transition protein expression & enhanced migration activity in colorectal cancer (CRC) cells. SW480 (low metastatic potential) & SW620 (high metastatic potential) cells were cultured in different concentrations of glucose (normal: NG; HG; & osmotic control: NG + l-glucose). (A) Morphological change occurred from epithelial to mesenchymal type in the HG-concentration group. (B) HG concentration caused downregulation of E-cadherin & upregulation of N-cadherin, beta CTN, & vimentin, but c-myc was unchanged, as detected using Western blotting. beta -actin was evaluated as an internal control. (C,D) Wound healing assay showed that HG concentration promoted cell motility in SW480 & SW620 CRC cells after 48 & 72 h of culture, compared with the NG & NG + l-glucose groups. (E) In a Transwell migration assay, 3.5 × 105 SW480 & SW620 CRC cells were plated onto a 24-well plate & cultured in NG & HG-concentration medium for 96 h. HG concentration promoted cell motility in SW480 & SW620 cells. NG + l-glucose cells were evaluated as ostomic controls. (F) These data show that HG concentration caused upregulation of p-IGF1R in CRC. In addition, HG concentration promoted IGF1R downstream signaling, including p-Src & p-ERK; these proteins were increased when CRC cells were cultured in HG-concentration medium. Levels of beta -actin were evaluated as loading controls. Statistically significant differences between the two groups were judged using Student’s t-tests; * p < 0.05, ** p < 0.005, *** p < 0.001; n.s. = nonsignificant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30965609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92687_mouse-monoclonal-vimentin-antibody-2d1-310202416113533.jpg)
![Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Simple-Western-NBP2-22127-img0006.jpg "Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunocytochemistry-Immunofluorescence-NBP2-22127-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and 72 hours following exposure to 0.4 uM GNE-617. Full-length western blots are shown in S2D Fig. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0164166), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0009.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunohistochemistry-Paraffin-NBP2-22127-img0005.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0008.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0007.jpg)
![ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-ELISA-NBP2-22127-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Western-Blot-NB400-134-img0005.jpg "Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.")
![Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-134-img0008.jpg)
![Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification]](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunohistochemistry-Paraffin-NB400-134-img0009.jpg)
![Flow Cytometry: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Flow-Cytometry-NB400-134-img0006.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0007.jpg "Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2525-img0006.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0008.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Thermal injury induces hepatic endoplasmic reticulum (ER) stress response in vivo. Western blots of ER stress proteins and ER calcium handling/chaperone proteins and/or their phosphoprotein levels in liver samples isolated from two control and six burned animals 24 hrs after burn injury. All Western blots are from the same animals. *Non-specific band. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/j.1582-4934.2008.00644.x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0009.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0005.jpg)
![Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Dual-RNAscope-ISH-IHC-NB400-156-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Immunocytochemistry-Immunofluorescence-NB400-156-img0007.jpg)
![Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Western-Blot-NB400-156-img0006.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (5C11) [H00004360-M02] - MRC1 monoclonal antibody (M02), clone 5C11. Analysis of MRC1 expression in human pancreas.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-5C11-Western-Blot-H00004360-M02-img0007.jpg "Western Blot: MMR/CD206/Mannose Receptor Antibody (5C11) [H00004360-M02] - MRC1 monoclonal antibody (M02), clone 5C11. Analysis of MRC1 expression in human pancreas.")
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (5C11) [H00004360-M02] - Analysis of monoclonal antibody to MRC1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-5C11-Immunohistochemistry-Paraffin-H00004360-M02-img0005.jpg)
![ELISA: MMR/CD206/Mannose Receptor Antibody (5C11) [H00004360-M02] - Detection limit for recombinant GST tagged MRC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-5C11-ELISA-H00004360-M02-img0008.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0011.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.")
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NBP1-30894-img0032.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0023.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0024.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0025.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0026.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0028.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0031.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020241959359.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma stroma. alpha smooth muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242011442.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242075975.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041219.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205699.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242063310.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420122078.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205673.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420104449.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041283.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-1610202419414539.jpg)
![Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Western-Blot-NB120-6401-img0011.jpg "Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Human pancreas cancer cells. Antibody at 1:250 dilution. Overnight incubation. No permeabilization. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunocytochemistry-Immunofluorescence-NB120-6401-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Staining of FFPE human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26, followed by Goat Anti Mouse IgG (Fab)-FITC.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Immunohistochemistry-Paraffin-NB120-6401-img0006.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of the subcutaneous xenografts in nude mice with H&E staining (left panel) and IHC with anti-pan CK (right panel) at 400x magnification. Asterisks (*) indicate representative keratinization and keratin-pearl formation. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0014.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of NHRI-HN1 tumors in nude mice and B6 mice with H&E staining (upper panels) at 400x and 1000x magnifications and IHC with anti-pan CK (middle panel) and EGFR (lower panel) at 400x magnification. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Detection of EMT & increased motility in NHRI-HN1 cells. (A) Summary of the most enriched pathways associated with tumorigenesis in syngeneic mice by comparing tumorigenic NHRI-HN1 cells with nontumorigenic cells, including M1-2, M2-3 & NHRI-HN2, using GSEA analysis. Blue indicates a negative normalized enrichment score (NES) & orange indicates a positive NES. (B) Morphology of M1-2 & NHRI-HN1 cells via phase-contrast microscopy at 100× magnification. (C) Relative adhesion activity in M1-2 & NHRI-HN1 cells, determined by normalizing the mean OD 490 nm value of NHRI-HN1 cells to that of M1-2 cells. (D) Cells stained with Alexa Fluor 488 phalloidin, anti-pan-CK & anti-EGFR antibodies at 400× magnification. (E) Immunoblot analysis of epithelial (E-cadherin, alpha -catenin & beta -catenin), mesenchymal (N-cadherin & Vimentin) proteins & EMT-related transcription factors, including Twist, Snail & Slug in M1-2 & NHRI-HN1 cells. Protein levels were normalized to an internal control, alpha -tubulin. Ratios were determined by dividing the normalized protein levels in NHRI-HN1 cells by that in M1-2 cells. (F) Representative images (left) & relative data (right) for migration activity of M1-2 & NHRI-HN1 cells. (G) Representative images (left) & relative data (right) for invasion activity of M1-2 & NHRI-HN1 cells at 200× magnification. The relative migration or invasion activity was determined by normalizing the mean number of cells that have migrated or invaded per field of NHRI-HN1 cells to that of M1-2 cells. Error bars represent SE; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31878324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6401_mouse-monoclonal-cytokeratin-pan-antibody-pck-26-310202416212383.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Knockdown-Validated-NB600-103-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: Calreticulin Antibody [NB600-103] - Lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Simple-Western-NB600-103-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-103-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - Analysis of FFPE human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in glands was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunohistochemistry-Paraffin-NB600-103-img0007.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB600-103 at 1:1,000. ECL exposure, 10 seconds.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0003.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0004.jpg)