| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38646SS | |||
| BSA Free | NBP3-38646 | |||
| BSA Free | NB100-690SS | |||
| Azide and BSA Free | NBP2-80570 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-144SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-506SS | |||
| Azide and BSA Free | NBP2-80571 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1639SS | |||
| Azide and BSA Free | NBP2-80569 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2216SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47254SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57308-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23602SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80849-25ul | |||
![Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Simple-Western-NB100-690-img0023.jpg "Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa.")
![Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with primary antibody at a 0.5ug/ml dilution for 15 min at RT.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Immunohistochemistry-NB100-690-img0028.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated with primary antibody for 15 min at a 5 ug/ml concentration. Corner image is staining with secondary only.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Immunohistochemistry-NB100-690-img0029.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Immunohistochemistry-NB100-690-img0031.jpg)
![Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Western-Blot-NB100-690-img0032.jpg)
![Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Flow-Cytometry-NB100-690-img0016.jpg)
![Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-DM1A-Immunomicroscopy-NB100-690-img0037.jpg)
![Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - GS treatment increases markers of beiging in 3T3-L1 adipocytes. GS treatment upregulates markers of beiging, including beta-3AR (C) proteins. Data presented as mean +/- SEM from n = 4 replicates per group. * p 0 for sensitive & <0 resistant. (D) Apoptotic H1299 (null p53), A549 (wild type p53), Bm7-shGFP (mutant p53), & Bm7-shTP53 (knock-down p53) cells treated with simvastatin were detected using flow cytometry, *P < 0.05 & **P < 0.01. (E) Apoptotic HCC827-shGFP (mutant p53) & HCC827-shTP53 (knock-down p53) cells treated with simvastatin & cisplatin were detected using flow cytometry, *P < 0.05 & **P < 0.01. (F) Western blots of indicated proteins involved in apoptosis & autophagy in both Bm7 & HCC827 cells with control (shGFP) & p53 knockdown (shTP53) treated with simvastatin is shown. MDM2, murine double minute 2; AKT, serine–threonine kinase; PARP, poly (ADP-ribose) polymerase; mTOR, mammalian target of rapamycin; WT, wild type. Full-length blots/gels are presented in Supplementary Fig. 1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31892709), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-690_mouse-monoclonal-alpha-tubulin-antibody-dm1a-img-80196-310202415541925.jpg)
![Western Blot: alpha Tubulin Antibody (DM1A) - BSA Free [NB100-690] - Pre-treatment with 0.5 mM sodium arsenite (SA) enhances permissivity in a cell-type-specific manner across reovirus strains. (A) CV-1, HeLa, L929, or HPDE cells were left untreated (no SA) or were treated with 0.5 mM SA for 30 min prior to infection (Pre-SA). Following this, cells were infected with T3D such that ~20% to 50% of cells were infected & at 18 h p.i. cells were fixed & immunostained for μNS & DAPI to visualize viral factories (VFs). The percent of cells containing VFs was quantified ((# of cells containing VFs/total # of cells) × 100) from three independent experiments. The expression level of μNS (B) & μ1 (C) was determined in CV-1, L929, or HeLa cells either left untreated (no SA) or treated with 0.5 mM SA for 30 min (Pre-SA) before infection with T3D at MOI = 1. At 18 h p.i., cells were harvested & the expression level of the indicated proteins was determined by immunoblot. M = mock. Densitometry analysis of the band intensity for μNS & μ1 was adjusted to the matched alpha -tubulin loading control for two independent experiments. Columns represent mean ± SEM. (D) CV-1; (E) L929; or (F) HeLa cells were left untreated (no SA) or were treated with 0.5 mM SA prior to infection (Pre-SA). Cells were then infected with the reovirus strains, T3D, T1L, or T3A, as described in (A). At 18 h p.i., cells were fixed & immunostained for μNS & DAPI to detect VFs. The percent of cells containing VFs was quantified ((# of cells containing VFs/total # of cells) × 100) from at least two independent experiments. * p < 0.05; ** p < 0.01; two-tailed unpaired t test. The error bars indicate S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31216693), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-690_mouse-monoclonal-alpha-tubulin-antibody-dm1a-img-80196-31020241553208.jpg)
![Immunohistological analysis of brain stem section stained with rabbit polyclonal Laminin Antibody [NB300-144], dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunohistochemistry-Free-Floating-NB300-144-img0018.jpg "Immunohistological analysis of brain stem section stained with rabbit polyclonal Laminin Antibody [NB300-144], dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.")
![MxIF analysis of mouse submandibular salivary gland morphogenesis. MxIF of a developmental TMA including embryonic stages (E14, E16, E18) and postnatal stages (P5 and P20) was performed using sequential application of directly conjugated antibodies to detect multiple markers of tissue structures and cell types on the same tissue sections. Tissue compartments. The epithelium, mesenchyme, neurons, and basement membranes was detected using antibodies directed towards E-cadherin (ECAD, red), platelet-derived growth factor (PDGFR, green), beta III tubulin (bIII, magenta), and Laminin Antibody [NB300-144] (LMN, cyan), respectively. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.20134309), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunohistochemistry-NB300-144-img0022.jpg)
![Staining of mouse section of cortex stained with Laminin Antibody [NB300-144] (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunohistochemistry-Free-Floating-NB300-144-img0014.jpg)
![Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using Laminin Antibody [NB300-144]. Pepsin antigen retrieval was performed on this tissue sample.](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunohistochemistry-NB300-144-img0008.jpg)
![Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) Laminin Antibody [NB300-144] recognizes 3 laminin isotypes: alpha 1 (440 kDa), beta 1 (220 kDa) and gamma 1 (220 kDa). Also recognized is a laminin binding protein at 120 kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant. Theoretical molecular weight of LAMA1 is 337 kDa.](http://images.novusbio.com/fullsize/Laminin-Antibody-Western-Blot-NB300-144-img0017.jpg)
![IF Confocal analysis of HeLa cells using Laminin Antibody [NB300-144] (1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-144-img0011.jpg)
![Western blot analysis of the effects of IP6, Ins, IP6 + Ins and normal saline on the levels of collagen IV, Laminin and Fibronectin, with Laminin Antibody [NB300-144]. IP6 or Ins treatment decreased the protein expression of LN and FN, and the combined IP6 + Ins treatment resulted in significantly greater effects compared with treatment with either compound alone. The Western blot membranes were stripped and reprobed for beta-actin as an internal control to confirm equal loading. (A) representative blots from one of three separate experiments; (B) relative band intensities based on densitometry. The results are expressed as the mean +/- standard deviation from three independent experiments. * p < 0.05 compared to the IP6 + Ins group; #p < 0.05 compared to the normal saline group. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/8/5/286), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Laminin-Antibody-Western-Blot-NB300-144-img0024.jpg)
![A surface stain was performed on HeLa cells with the Alexa Fluor 647 conjugate of Laminin Antibody [NB300-144AF647] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Laminin-Antibody-Flow-Cytometry-NB300-144-img0020.jpg)
![Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right), using Laminin Antibody [NB300-144]. The Laminin polyclonal antibody was used at 1 ug/mL. Theoretical Molecular Weight of NB300-144 is 337 kDa.](http://images.novusbio.com/fullsize/Laminin-Antibody-Western-Blot-NB300-144-img0010.jpg)
![VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) using Laminin Antibody [NB300-144] shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Laminin-Antibody-Immunohistochemistry-NB300-144-img0023.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - hS/PCs sequentially secrete basement membrane proteins during microstructure reorganization & function. Encapsulated hS/PCs in HA-PEGDA hydrogels initially produce laminin (red) (A–C) & collagen IV (green) (D–F) even at the single-cell state (A,D). Perlecan (green) (G–I) secretion follows to stabilize the laminin & collagen IV networks. Scalebar = 20 μm in all confocal micrographs. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31750298), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-144_rabbit-polyclonal-laminin-antibody-310202415171342.jpg)
![Western Blot: Laminin Antibody [NB300-144] - Western blot analysis of the effects of IP6, Ins, IP6 + Ins & normal saline on the levels of collagen IV, Lamininand Fibronectin. IP6 or Ins treatment decreased the protein expression of collagen IV, LN & FN, & the combined IP6 + Ins treatment resulted in significantly greater effects compared with treatment with either compound alone. The samples were probed with antibodies against p-collagen IV, p-LN, & p-FN. The Western blot membranes were stripped & reprobed for beta -actin as an internal control to confirm equal loading. (A) representative blots from one of three separate experiments; (B) relative band intensities based on densitometry. The results are expressed as the mean ± standard deviation from three independent experiments. * p < 0.05 compared to the IP6 + Ins group; #p < 0.05 compared to the normal saline group. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/8/5/286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-144_rabbit-polyclonal-laminin-antibody-310202416212338.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0021.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - NIH3T3 cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-NB600-506-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Rat FR cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0023.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0015.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunohistochemistry-Paraffin-NB600-506-img0005.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0017.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0019.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0002.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0013.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0012.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0016.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Tissue levels of VEGF & PCNA.VEGF levels were estimated by (A) western blot & (B) ELISA. (C) PCNA levels estimated by western blot. Data were normalized with tubulin. (D) Representative images of PCNA immunohistochemistry staining in the nucleus is shown for CS-exposed mice (CSE) & CS-exposed & LGF-treated mice (CSE+LGF). Scale bars = 50 µm. LGF promoted an increase of PCNA+ cells as showed in the bar graph. ** P<0.01 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-310202415304283.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - LGF ameliorates oxidative stress.Bars represent (A) 3NT & (B) Nrf2 levels estimated by western blot. Data were normalized with tubulin. * P<0.05 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241539740.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Tissue levels of VEGF & PCNA.VEGF levels were estimated by (A) western blot & (B) ELISA. (C) PCNA levels estimated by western blot. Data were normalized with tubulin. (D) Representative images of PCNA immunohistochemistry staining in the nucleus is shown for CS-exposed mice (CSE) & CS-exposed & LGF-treated mice (CSE+LGF). Scale bars = 50 µm. LGF promoted an increase of PCNA+ cells as showed in the bar graph. ** P<0.01 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241534356.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - LGF ameliorates oxidative stress.Bars represent (A) 3NT & (B) Nrf2 levels estimated by western blot. Data were normalized with tubulin. * P<0.05 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-310202415284516.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Role of cell-type-specific cyclooxygenase-2 (Cox-2) expression in Ras/p53-mediated tumor formation. a Experimental scheme. b Microscopic phenotype & histology of three-dimensional (3D) organoids with/without celecoxib treatment. c Relative 3D organoid formation was determined by the number of organoids larger than 100 µm, n = 3 independent experiments, 25 fields at high power field (HPF) per sample. Veh, vehicle control; C25, 25 µM; C50, 50 µM celecoxib. d Experimental scheme. e Ras/p53-mediated tumor incidence with/without condition Cox-2 knockout. Ptgs2wt/wt, n = 15 animals; Ptgs2flox/flox, n = 14 animals. f, g Histological phenotypes. h–k Immunoblotting of Keratin5 (Krt5), Krt6 & Loricrin (Lor) demonstrated decreased tumor susceptibility but increased differentiation status, n = 4 independent experiments. l Summary of the contribution of physiological stress factors in the susceptibility of tumor development from tumor-competent Krt15+ progenitors. Data with bar graphs are represented as mean ± SEM. Statistical significance was determined by pair-wise comparison using t test; ns = not significant, *p < 0.05, **p < 0.005. Scale bars, 100 µm. Ptgs2 prostaglandin-endoperoxide synthase 2 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31110179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241616019.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Microenvironmental acidic stressors & tumor susceptibility. a Experimental scheme. Epithelial cells isolated from the regions adjacent or distant from squamocolumnar junction (SCJ). b Microscopic phenotype of three-dimensional (3D) organoids from control & Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice. Histology of 3D organoids demonstrated that oncogenic Ras/p53 increased abnormal growth features. c–e Quantification of relative organoid formation (n = 3 independent experiments, 25 fields at high power field (HPF) per sample) & size distribution (n ≥ 150) from control & experimental mice expressing oncogenic Ras/p53 combination. f Experimental scheme. Phosphate-buffered saline (PBS) (vehicle) or PPI were daily treated by intraperitoneal (i.p.) injections (5 consecutive days per week). g Tumor incidence from Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice, with/without PPI treatment, n = 9 animals per each. Statistical significance was determined by Fisher’s exact test; *p < 0.05. h–j Immunoblotting of Keratin5 (Krt5) & Krt6 demonstrated relatively less tumor formation in the PPI treatment group, n = 5 independent experiments. k–m Immunostaining of Krt5, Krt6, & phospho-histone H3 (ph-H3), & histology demonstrated suppressed tumor formation by daily PPI treatment in Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice. PPI, proton pump inhibitor. Data with bar graphs are represented as mean ± SEM. Statistical significance was determined by pair-wise comparison using t test; *p < 0.05, **p < 0.005. Scale bars, 100 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31110179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241618944.jpg)
![Western Blot: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - Total protein from human HeLa and A431, mouse 3T3 and rat PC12 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-alpha Tubulin in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Western-Blot-NB100-1639-img0008.jpg "Western Blot: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - Total protein from human HeLa and A431, mouse 3T3 and rat PC12 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-alpha Tubulin in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Alpha tubulin molecular weight: 50 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YOL1/34) - BSA Free [NB100-1639] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with alpha Tubulin Antibody [YOL1/34] (NB100-1639) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1639-img0014.jpg)
![Flow (Intracellular): alpha Tubulin Antibody (YOL1/34) [NB100-1639] - An intracellular stain was performed on HeLa cells with Tubulin [YOL1/34] Antibody NB100-1639 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Flow-Intracellular-NB100-1639-img0007.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody [YOL1/34] NB100-1639AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Flow-Cytometry-NB100-1639-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tubulin Antibody [YOL1/34] at 5 ug/ml overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Immunocytochemistry-Immunofluorescence-NB100-1639-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YOL1/34) - BSA Free [NB100-1639] - alpha Tubulin Antibody (YOL1/34) [NB100-1639] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tubulin Antibody [YOL1/34] at 5 ug/ml overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Immunocytochemistry-Immunofluorescence-NB100-1639-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tubulin Antibody [YOL1/34] at 5 ug/ml overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Immunocytochemistry-Immunofluorescence-NB100-1639-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - Mutants unable to ubiquitinate histone H2B-K123 display growth and Cdc14 release defects when combined with MEN mutants. Representative images from Cdc14 release assay. Cells are assayed for spindle morphology and Cdc14 release phenotype by indirect immunofluorescence with anti-tubulin or anti-HA, which recognizes the HA-tagged version of Cdc14 in all strains. Cells are visualized by nuclear staining with DAPI and by differential interference contrast (DIC) optics for comparison. No release refers to dense nucleolar staining of Cdc14-HA, full release refers to cells with uniform Cdc14-HA localization throughout the nucleus, while partial release refers to cells with both nuclear and residual nucleolar staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1000588), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YOL1-34-Immunocytochemistry-Immunofluorescence-NB100-1639-img0012.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NB100-2216-img0005.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2216-img0006.jpg)
![Western Blot: Filamin A Antibody (4E10-1B2) [H00002316-M01] - FLNA monoclonal antibody (M01), clone 4E10-1B2 Analysis of FLNA expression in HL-60.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-4E10-1B2-Western-Blot-H00002316-M01-img0009.jpg "Western Blot: Filamin A Antibody (4E10-1B2) [H00002316-M01] - FLNA monoclonal antibody (M01), clone 4E10-1B2 Analysis of FLNA expression in HL-60.")
![Immunohistochemistry-Paraffin: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of monoclonal antibody to FLNA on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-4E10-1B2-Immunohistochemistry-Paraffin-H00002316-M01-img0007.jpg)
![Proximity Ligation Assay: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of protein-protein interactions between GP1BA and FLNA. HeLa cells were stained with anti-GP1BA rabbit purified polyclonal 1:1200 and anti-FLNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Filamin-A-Antibody-4E10-1B2-Proximity-Ligation-Assay-H00002316-M01-img0005.jpg)
![ELISA: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Filamin A Antibody (4E10-1B2) [H00002316-M01] - Detection limit for recombinant GST tagged FLNA is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-4E10-1B2-ELISA-H00002316-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57308-img0001.jpg "Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunohistochemistry-Paraffin-NBP2-57308-img0018.jpg)
![Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunohistochemistry-Paraffin-NBP2-57308-img0014.jpg)
![Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunohistochemistry-Paraffin-NBP2-57308-img0015.jpg)
![Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunohistochemistry-Paraffin-NBP2-57308-img0016.jpg)
![Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human liver shows moderate to strong membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Immunohistochemistry-Paraffin-NBP2-57308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg)
![Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80849 (A) shows similar protein distribution across tissues to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80849] - Analysis using Anti-RGN antibody NBP1-80849 (A) shows similar pattern to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0019.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0027.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80849] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0004.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0025.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0026.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0028.jpg)
![Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Western-Blot-NBP1-40067-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0003.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Analysis of anti-PTH1R antibody with human liver at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-Paraffin-NBP1-40067-img0001.jpg)
![Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-310202415175227.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-31020241534388.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0007.jpg "Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0001.jpg)
![Immunoprecipitation: alpha Tubulin Antibody (TU-01) [NB500-333] - alpha Tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Molecular weight of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunoprecipitation-NB500-333-img0006.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of alpha-tubulin (molecular weight of 50 kDa) in porcine brain lysate by antibody TU-01 biotin.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Tubulin filament in HeLa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0005.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of lysates of various cell lines by a newly developed monoclonal antibody (B). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0002.jpg)
