| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-119SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68136SS | |||
| Azide and BSA Free | NBP2-80973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB300-101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97417SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71774SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22444SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-543-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75522-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44999SS | |||
![Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0009.jpg "Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.")
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-119-img0004.jpg)
![Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunohistochemistry-NB300-119-img0006.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0010.jpg)
![Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-119_rabbit-polyclonal-nk1r-antibody-immunocytochemistry-immunofluorescence-1372023145313..jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - TACR1 & its downstream targets SRC & p-SRC are expressed in neuroblastoma cell lines(A) Endogenous TACR1, SRC & p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. (B) Quantitative RT-PCR of TACR1 mRNA in a panel of neuroblastoma cell lines (n = 3, error bars indicate standard deviation). (C–E) Quantification of SRC (C), p-SRC (D) & TACR1 (E) protein expression using densitometry analysis of western immunoblots (n = 3, error bars indicate standard deviation). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202415392524.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416205147.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416212385.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Intracellular-NB110-68136-img0010.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![<b>Biological Strategies Validation. </b>Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-BSA-Free-Flow-Cytometry-NB110-68136-img0016.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0008.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0007.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0011.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0013.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0007.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.")
![Immunohistochemistry: Neurokinin 1 Receptor Antibody [NB300-101] - Immunohistochemical analysis of Neurokinin 1 Receptor in mouse brain (selected cells, axon & dendrites) using NB300-101.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunohistochemistry-NB300-101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0010.jpg)
![Western Blot: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - Analysis of TRPV1 in following cell lysates: (1) Ntera2, (2) HepG2, (3) MCF7, and (4) Cos7.](http://images.novusbio.com/fullsize/TRPV1-Antibody-Western-Blot-NBP1-97417-img0002.jpg "Western Blot: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - Analysis of TRPV1 in following cell lysates: (1) Ntera2, (2) HepG2, (3) MCF7, and (4) Cos7.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - TRPV1 Antibody [NBP1-97417] - TRPV1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/TRPV1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97417-img0003.jpg)
![Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - The Vanilloid R1/TRPV1+ and Vanilloid R1/TRPV1+CD4+ immune cells in patients with allergic rhinitis. Immunohistochemical staining of Vanilloid R1/TRPV1 (single staining) and CD4/Vanilloid R1/TRPV1 (double staining) in patients with allergic rhinitis compared with control subjects. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6653), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-NBP1-71774-img0010.jpg "Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - The Vanilloid R1/TRPV1+ and Vanilloid R1/TRPV1+CD4+ immune cells in patients with allergic rhinitis. Immunohistochemical staining of Vanilloid R1/TRPV1 (single staining) and CD4/Vanilloid R1/TRPV1 (double staining) in patients with allergic rhinitis compared with control subjects. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6653), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - TRPV1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71774-img0007.jpg)
![Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - Analysis of TRPV1 in mouse dorsal root ganglion using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-NBP1-71774-img0008.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - Human stomach tissue. Positive staining can be observed in gastric neurons. Image from verified customer review.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NBP1-71774-img0009.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0014.jpg "Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.")
![Immunohistochemistry-Paraffin: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Paraffin-NBP1-22444-img0011.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0012.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/acel.13134) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0013.jpg)
![Immunohistochemistry-Frozen: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Frozen-NBP1-22444-img0010.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Age attenuates the role of CaV3.2 channels in Ca2+ spark generation & decreases CaV3.2 protein expression in VSMC. (a), Ca2+ fluorescence images of a Fluo‐4‐AM–loaded VSMC from a young mouse & time course of Ca2+ fluorescence changes in the cellular ROI (upper panel). Cell boundary is marked with dashed line. (b), same as (a) but in the presence of Ni2+ (50 µM). (c), same as (a) but in a VSMC from an old mouse. (d), same as (c) but in the presence of Ni2+ (50 µM). (e, f), summary of the results. Ca2+ spark frequency (e) & fraction of cells producing Ca2+ sparks (f) in VSMCs from young mice (n = 102), in VSMCs from young mice cells incubated with Ni2+ (n = 85), in VSMCs from aged mice (n = 129), & in VSMCs from aged mice cells incubated with Ni2+ (n = 127). Cells were isolated from 4 mice in each group; 25–40 cells were recorded & analyzed from each mouse. VSMC, vascular smooth muscle cell. (g), Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. n.s., not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32187825), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-22444_mouse-monoclonal-cav3-2-antibody-s55-10-31020241617145.jpg)
![Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) [NB300-543] - Biopsies of deparaffinized Human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Ryanodine-Receptor-1-Antibody-34C-Immunohistochemistry-Paraffin-NB300-543-img0001.jpg "Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) [NB300-543] - Biopsies of deparaffinized Human skeletal muscle tissue.")
![Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886] - The expressed and electrophysiology properties of Vanilloid R1/TRPV1 in primary cultured microglia. Representative Immunoblot bands and densitometric analysis of total protein (E) and membrane protein expression (F) of Vanilloid R1/TRPV1 in cortical primary cultured microglia with or without pretreatment of CAP (10 uM), hyperthermia (43C, 4*30 min) or LPS (1.0 ug/ml, 24 h). Data were presented as means +/- SEM, n = 3 per groups, *p < 0.05, **p < 0.01, one-way analysis of variance (ANOVA) followed by Dunnett's multiple comparison test with DMSO. Ctrl, Control; CAP, capsaicin; HT, hyperthermia. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Western-Blot-NB100-98886-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886] - The expressed and electrophysiology properties of Vanilloid R1/TRPV1 in primary cultured microglia. Representative Immunoblot bands and densitometric analysis of total protein (E) and membrane protein expression (F) of Vanilloid R1/TRPV1 in cortical primary cultured microglia with or without pretreatment of CAP (10 uM), hyperthermia (43C, 4*30 min) or LPS (1.0 ug/ml, 24 h). Data were presented as means +/- SEM, n = 3 per groups, *p < 0.05, **p < 0.01, one-way analysis of variance (ANOVA) followed by Dunnett's multiple comparison test with DMSO. Ctrl, Control; CAP, capsaicin; HT, hyperthermia. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Vanilloid R1/TRPV1 and toll-like receptor 4 (TLR4) mutually inhibited the transforming growth factor-beta1 (TGF-beta1) signaling in activated microglia. Photomicrograph immunocytochemistry indicated different co-expression of Vanilloid R1/TRPV1 (visualized in red Cy3), TLR4 (visualized in green DyLight 488), and cell nuclei (visualized in dark blue DAPI) in activated microglia. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98886-img0010.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Transient receptor potential vanilloid type 1 (Vanilloid R1/TRPV1) protein expression pattern in cortical microglia after rFS. Western blot analysis of total protein (A) and membrane protein expression (B) of Vanilloid R1/TRPV1 in three samples from rFSs group's mice. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Western-Blot-NB100-98886-img0008.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0002.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0003.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0004.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0005.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98886-img0006.jpg)
![Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Orai1-Antibody-3F6H5-Western-Blot-NBP1-75522-img0001.jpg "Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunofluorescence of Orai1 in human spleen tissue with Orai1 antibody at 20 u/ml.Red: Orai1 Antibody [3F6H5] Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-75522_mouse-monoclonal-orai1-antibody-3f6h5-271120241923622.gif)
![Immunocytochemistry/ Immunofluorescence: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunofluorescence of Orai1 in human spleen tissue with Orai1 antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp1-75522_mouse-monoclonal-orai1-antibody-3f6h5-2711202420324820.jpg)
![Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-75522_mouse-monoclonal-orai1-antibody-3f6h5-2711202419251517.gif)
![Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-75522_mouse-monoclonal-orai1-antibody-3f6h5-2711202420195396.jpg)
![Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours at room temperature at 1:5000. (Data provided by Karen McColl at Case Western Reserve University)](http://images.novusbio.com/fullsize/ITPR2-Antibody-Western-Blot-NB100-2466-img0001.jpg "Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours at room temperature at 1:5000. (Data provided by Karen McColl at Case Western Reserve University)")
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0004.jpg)
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0002.jpg)
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0003.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Western-Blot-NBP1-44999-img0009.jpg "Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.")
![Immunocytochemistry/Immunofluorescence: HOMER1 Antibody [NBP1-44999] - Detection of HOMER1 (Green) in Hela cells using NBP1-44999. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44999-img0007.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on mouse brain lysate.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Western-Blot-NBP1-44999-img0008.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0014.jpg "Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116] - FaDu xenograft, using Caveolin 2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31116-img0006.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0008.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0013.jpg)
![Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ug Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Immunoprecipitation-NBP1-31116-img0012.jpg)
