Ryanodine Receptor Antibody (34C) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Rt, Am, Bv, Ca, Fi, Ma, Ma-Mn, Pm, Rb, Sh
• Applications: WB, ICC/IF, IHC, IP, B/N
• Clone: 34C
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Ryanodine Receptor Antibody (34C) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Partially purified chicken pectoral muscle ryanodine receptor.
• Specificity: This antibody detects ryanodine receptor Ryanodine Receptor-1 and Ryanodine Receptor-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chicken, it detects the alpha, beta and cardiac isoforms. This antibody also detects Ryanodine Receptor-3 in mouse cells. In frog, it detects the alpha and beta isoforms and in fish it detects the alpha isoform.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RYR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Ryanodine Receptor Antibody (34C) [CoraFluor™ 1]

• ARVC2
• ARVD2
• cardiac muscle ryanodine receptor-calcium release channel
• cardiac-type ryanodine receptor
• CCO
• central core disease of muscle
• islet-type ryanodine receptor
• kidney-type ryanodine receptor
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• ryanodine receptor 1
• ryanodine receptor 2 (cardiac)
• ryanodine receptor 2
• RYDR
• RYR
• RYR-1
• RYR-2
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• SKRR
• type 1-like ryanodine receptor
• type 2 ryanodine receptor
• VTSIP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYR1