| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-582SS | |||
| Azide and BSA Free | NBP2-80959 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-74555-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2215SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05767SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2216SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
![Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Western-Blot-NB600-582-img0004.jpg "Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license.")
![Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Western-Blot-NB600-582-img0002.jpg)
![Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Simple-Western-NB600-582-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, and WT Bel-7402 cells. Data were expressed as mean -/+ standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT group, respectively.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Knockout-Validated-NB600-582-img0005.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the expression of Nrf2‐related signalling proteins in OA (660 μmol/L) & PA (330 μmol/L)‐induced HepG2 cells. GEN was added into HepG2 cells prior 1 h to stimulation of OA & PA for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm was detected by Western blot. (D & E) Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta & AMPK beta was detected by Western blot. F‐H, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control Group; *P < .05 & **P < .01 vs OA & PA Group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-31020241534375.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - KO & OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. (A) Total cellular TG content of SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (B) HPLC-MS determination of the SFAs to MUFAs ratios (palmitic acid to palmitoleic acid, 16:0 to 16:1) (stearic acid to oleic acid, 18:0 to 18:1) as well as the content of 16:0, 16:1, 18:0, & 18:1 in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (C) The transcript level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (D & E) The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. Data were expressed as mean ± standard error of the mean. *P < 0.05, **P < 0.01, & ***P < 0.001 vs. WT group, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202415384185.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Construction of knockout (KO) & overexpression (OE) cell lines. (A) The sequencing results of KO positive clone cells; (B) The messenger RNA (mRNA) level of SREBP1 in wild type (WT) & OE Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group; (C & D) The protein level of SREBP1 in WT, OE, & KO Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-31020241539748.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 & SREBP2. a SREBP1, immunoblots & b quantification. Values are mean ± SD, n = 3. **P < 0.01 & *p < 0.05 for treated vs. control cells. c, d SREBP2. Cells were treated with 10 ng/ml pro-NGF in the absence or presence of 0.5 μM PF429242 (PF), which is a selective inhibitor of the Site 1 protease (S1P). c Immunoblots & d quantification. PF decreased the amount of processed SREBP2 in these cells; however, addition of pro-NGF increased the relative level of cleaved SREBP2. Values are mean ± SD, n = 3. *p < 0.05 for treated vs. the corresponding control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31296846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416161836.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 & SREBP2. a SREBP1, immunoblots & b quantification. Values are mean ± SD, n = 3. **P < 0.01 & *p < 0.05 for treated vs. control cells. c, d SREBP2. Cells were treated with 10 ng/ml pro-NGF in the absence or presence of 0.5 μM PF429242 (PF), which is a selective inhibitor of the Site 1 protease (S1P). c Immunoblots & d quantification. PF decreased the amount of processed SREBP2 in these cells; however, addition of pro-NGF increased the relative level of cleaved SREBP2. Values are mean ± SD, n = 3. *p < 0.05 for treated vs. the corresponding control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31296846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416175210.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the protein expression of Nrf2, ACC & mTORC signalling pathways in tyloxapol‐induced mice. GEN (50, 75, 100 mg/kg) was administered to WT or Nrf2−/− mice prior 1 h to stimulation of tyloxapol (500 mg/kg) for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm of WT mice was detected by Western blot. (D & E) Protein expression of Nrf2, PPAR alpha & PPAR gamma in Nrf2−/− mice was detected by Western blot. F‐H, Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta , AMPK beta in WT & Nrf2−/− mice was detected by Western blot. I‐M, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 WT & Nrf2−/− mice was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control group; *P < .05 & **P < .01 vs Tyloxapol group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416171460.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Paraffin-NB100-74555-img0009.jpg "Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.")
![Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NB100-74555-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74555-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74555-img0006.jpg)
![Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Free-Floating-NB100-74555-img0008.jpg)
![Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunoprecipitation-NB100-74555-img0007.jpg)
![Simple Western: SREBP1 Antibody [NB100-2215] - Image shows a specific band for SREBP1 in 1 mg/mL of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Simple-Western-NB100-2215-img0007.jpg "Simple Western: SREBP1 Antibody [NB100-2215] - Image shows a specific band for SREBP1 in 1 mg/mL of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-2215] - CDC2 associates with SPDY at ERES. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), and SPDY (B, red). Both CDC2 and SPDY localize to the same cortical domain (C). Note that the center of this oocyte is dented (the area that contains the arrow) causing the structure to appear in the middle of the oocyte, whereas it is located in the cortex. Images are Z-projections of 2 (A-C); scale bars represent 20 um in C and 5 um in C'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (B'-C'). Arrowheads denote the position of the GV. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-9-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2215-img0011.jpg)
![Western Blot: SREBP1 Antibody [NB100-2215] - MiR-181a overexpression impairs and miR-181a inhibition improves glucose and lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1. 2 mM NEFA), and miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors and then treated with 1. 2 mM NEFA). It was followed with or without 100 nM insulin. Immunoblot analysis (left) and quantification (right) of SREBP-1c and PPARI+- in HepG2 cells. Image collected and cropped by Citeab from the following publication (Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis. Oncotarget (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Western-Blot-NB100-2215-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-2215] - SREBP1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Nuclear and punctate vesicle staining was observed.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2215-img0006.jpg)
![Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - nalysis of FFPE tissue section of mouse liver with SREBP1 antibody at 1:200. The antibody generated an expected cytoplasmic-nuclear immunostaining of SREBP1 protein in the hepatocytes.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunohistochemistry-Paraffin-NB100-2215-img0008.jpg)
![Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1. Lane 1: human liver. Lane 2: mouse liver.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Western-Blot-NB100-2215-img0004.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - MiR-181a overexpression impairs & miR-181a inhibition improves glucose & lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1.2 mM NEFA), & miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors & then treated with 1.2 mM NEFA). B was followed with or without 100 nM insulin. (A) Immunoblot analysis (top) & quantification (bottom) of SIRT1, PGC-1 alpha & acetylated PGC-1 alpha in HepG2 cells. (B) Immunoblotting analysis (top) & quantification (bottom) of insulin-stimulated phosphorylation of AKT in HepG2 cells. (C) Glucose concentration in medium. (D) The glycogen content in HepG2 cells. (E) The mRNA expression levels of G6Pase & PEPCK. (F) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha in HepG2 cells. (G) TG content in HepG2 cells. *P 25 (n = 24). There was no significant difference. (F) The BMI and H-scores of PPAR alpha , SREBP1, and FAS in each EMC patient are shown in scatter plots. Spearman’s rank correlation coefficient showed a weak inverse correlation between BMI and the PPAR alpha H-score and a moderate or weak correlation between BMI and SREBP1 or FAS. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37305395), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-immunohistochemistry-13220261944580.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0002.jpg "Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.")
![Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/DIO3-Antibody-Flow-Cytometry-NBP1-05767-img0005.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0003.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NB100-2216-img0005.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2216-img0006.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - NR and Metf promote FoxO1-mediated Lipa upregulation in visceral AT of adult mice. Adult C57/BL6 mice (5 months) were nutrient restricted (NR) by 24 h fasting or treated for 10 days with Metf (400 mg/kg) dissolved in drinking water (n=4 mice per group). Western blot of FoxO1 and Lipa in total protein extracts of explanted visceral (epididymal) AT. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013404) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0009.jpg)
![Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Simple-Western-NBP1-54155-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody NBP1-54155 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54155-img0010.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free [NBP1-54155] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (NBP1-54155). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lipase-A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-54155-img0011.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0001.jpg)
![Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP1-54155-img0005.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416154610.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416165629.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - AMPK drives Lipa-released FFAs oxidation restraining energetic catastrophe. (a) 3T3-L1 cells were transfected with DN-AMPK or empty vector. RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha , carnitine palmitoyltransferase 1b & acyl-CoA oxidase 1 mRNA levels were performed after 4 h of NR or 16 h of Metf treatment. Dashed line indicates the mRNA value of untreated DN-AMPK cells (Ctr). mRNA levels of untreated cells transfected with empty vector were similar to untreated DN-AMPK cells (data not shown). (b) Cheminoluminescent assay of ATP level in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector after 8 h NR or 16 h Metf treatment. ATP level was expressed as pmol ATP per mg protein. (c) After 8 h of NR or 16 h Metf treatment, FFAs were enzymatically detected in culture medium of 3T3-L1 adipocytes transfected with DN-AMPK or empty vector. Values were expressed as μg FFAs per mg protein. (d) Left panel: western blot of AMPKpT172, PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 8 h NR. Right panel: cytofluorimetric analysis of apoptosis in DN-AMPK cells subjected to 8 h NR. (e) Western blot of PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & treated with Metf for 16 h. (f) Western blot of FoxO1, Lipa, LC3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 4 h NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05, °°P<0.01 versus Metf treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241618936.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171493.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171436.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - Lipa downregulation impairs lipid breakdown & elicits cell death in nutrient restricted adipocytes. (a) 3T3-L1 adipocytes were transfected with siRNA against Lipa (Lipa(−)) or with a scramble siRNA (Scr). Western blot of Lipa, PARP-1 & cleaved form of caspase-3 in total protein extracts from 3T3-L1 adipocytes after 4 h of NR. (b) TG content was quantified by ORO staining in fixed 3T3-L1 adipocytes 6 h after NR. (c) RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha & carnitine palmitoyltransferase 1b mRNA levels was performed in 3T3-L1 adipocytes 4 h after NR. (d) FFAs were analyzed in culture medium 6 h after NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05 versus NR treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416163713.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241616083.jpg)
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31944-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NBP1-31944-img0009.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunohistochemistry-Paraffin-NBP1-31944-img0004.jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-202202482557..jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020242011458.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020241957213.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-1610202419414513.jpg)
![Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-141120251833591.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-141120251839311.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - RT2 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0013.jpg "Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - RT2 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - U87 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0011.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0012.jpg)
![Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-NBP1-31386-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31386_rabbit-polyclonal-dopamine-beta-hydroxylase-antibody-1610202419405733.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)