Lipid-and-Metabolism reagents: Novus Biologicals

NB400-114

Fatty Acid Synthase/FASN Antibody - BSA Free

(2 Reviews)

(29 Publications)

0.1 ml

NB400-114

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY license.

<b>Genetic Strategies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed and open symbols indicate replicates from the primary screen and during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d and in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC and 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) and ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b and d where representative images are shown and for g where the mean+/-s.d. is shown for each point of a representative experiment, all data represent the means+/-s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB400-114SS

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NBP2-13172

GADD153/CHOP Antibody - BSA Free

(1 Review)

(27 Publications)

0.1 ml

NBP2-13172

<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. Western blot assays showing the effects of psoralen and thapsigargin on ER-stress related protein expression. Image collected and cropped by CiteAb from the following publication (//biolres.biomedcentral.com/articles/10.1186/s40659-019-0241-8), licensed under a CC-BY license.

Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - Mouse liver tissue. Antigen retreival: Citrate buffer pH=6.0,Blocking: 10% BSA in 0.1%Tween in TBS, Primary antibody: 1:100 in 1% BSA in 0.1%Tween in TBS,Incubation: Overnight at 4C, Secondary antibody: 1:200 incubation for 1hr at room temperature, AB complex: 1:100 for 1hr at room temprature Develpment DAB. IHC-P image submitted by a verified customer review.

Flow Cytometry: GADD153/CHOP Antibody [NBP2-13172] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody NBP2-13172F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.

Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.

Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. a Western blot assays showing the effects of psoralen & thapsigargin on ER-stress related protein expression. b Western blot assays showing the effects of TUDC on the expression of ER-stress related protein induced by PSO. c The mRNA levels of GRP78 & DDIT3 with TUDC under PSO & TG treatment. Values are mean ± SD (n = 3) & * is means compared to the Con group, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31277690), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Reduction of fibronectin prevents Dex-induced ER stress in human GTM-3 cells. (A) GTM3 cells were transfected with a plasmid expressing CRISPR-Cas9 targeting fibronectin (CR-FN) & then treated with Veh or Dex for 48 hours. Fibronectin knockdown partially reduced ER stress as evident from reduced GRP78 & CHOP levels after Dex treatment (n = 2). (B) Human GTM3 cells were treated with Dex (100 nM) with or without CR-FN for 48 hours. Fixed cells were stained for fibronectin & KDEL to examine Dex-induced fibronectin & ER stress. Fibronectin knockdown reduced fibronectin & ER stress in Dex-treated TM cells (n = 2). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - TM cells are sensitive to ER stress from Dex-induced abnormal ECM compared to other ocular cells: Primary human TM cells (A), RPE (B) & corneal fibroblasts (C) were treated with Veh or Dex for 3 days & immunostained for fibronectin & KDEL. Although fibronectin was increased in all of these cell types, ER stress was only observed in TM cells. (D) Human GTM3 or ARPE-19 cells were treated with Veh or Dex for 3 days. Fibronectin & chronic ER stress markers ATF4 & CHOP were examined in cellular lysates (n = 2). Although Dex increased fibronectin in both GTM3 & ARPE-19 cells, chronic ER stress was only observed in GTM3 cells indicating that TM cells are more prone to ER stress from Dex-induced ECM accumulation. (E) GTM-3 or ARPE-19 cells were treated with cellular fibronectin (cFn) for 2 days & ER stress was examined by Western blot analysis. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Overexpression of extracellular fibronectin induces ER stress in primary human TM cells. (A) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & cellular lysates were analyzed for ER stress markers. (B) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & fixed cells were stained with fibronectin & KDEL antibodies. Cellular fibronectin increased extracellular fibronectin & ER stress in GTM3 cells (n = 2). Scale bar = 50 μm. (C) GTM3 cells were transduced with fibronectin activation lentiviral particles for 48 hours & stained with fibronectin & KDEL antibodies. Increased fibronectin expression alone is sufficient to induce ER stress in TM cells (n = 2). Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-13172AF350
Alexa Fluor 405	NBP2-13172AF405
Alexa Fluor 488	NBP2-13172AF488
Alexa Fluor 532	NBP2-13172AF532
Alexa Fluor 594	NBP2-13172AF594
Alexa Fluor 647	NBP2-13172AF647
Alexa Fluor 700	NBP2-13172AF700
Alexa Fluor 750	NBP2-13172AF750
Allophycocyanin	NBP2-13172APC
Biotin	NBP2-13172B
CoraFluor 1	NBP2-13172CL1
DyLight 350	NBP2-13172UV
DyLight 405	NBP2-13172V
DyLight 488	NBP2-13172G
DyLight 550	NBP2-13172R
DyLight 594	NBP2-13172DL594
DyLight 650	NBP2-13172C
DyLight 680	NBP2-13172FR
DyLight 755	NBP2-13172IR
FITC	NBP2-13172F
HRP	NBP2-13172H
Janelia Fluor 525	NBP2-13172JF525
Janelia Fluor 549	NBP2-13172JF549
Janelia Fluor 646	NBP2-13172JF646
Janelia Fluor 669	NBP2-13172JF669
PE	NBP2-13172PE
PerCP	NBP2-13172PCP
mFluor Violet 450 SE	NBP2-13172MFV450
mFluor Violet 500 SE	NBP2-13172MFV500
mFluor Violet 610 SE	NBP2-13172MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-13172-0.025ml

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NB110-41576

Abhd5 Antibody - BSA Free

(29 Publications)

0.1 ml

NB110-41576

Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.

Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Protein content of important players involved in lipid droplet dynamics in Caucasian and South Asian subjects before and after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 and (e) PLIN5 protein content. Data are presented as mean ± SEM and were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect.

The turnover of DAG and TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) and ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - A. ATGL (n = 8) & CGI-58 (n = 7) protein content in the three skeletal muscles (SOL, RG, & WG & heart).Ponceau stain for total protein was used as a loading control. Data are reported as mean±SE & bars with the same letter are not significantly different within the skeletal muscles (p<0.05). Insets: representative blots: lane 1, WG (white gastrocnemius); lane 2, RG (red gastrocnemius); lane 3, SOL (soleus). B. ATGL & CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - The turnover of DAG & TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) & ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the right ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Protein content of important players involved in lipid droplet dynamics in Caucasian & South Asian subjects before & after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 & (e) PLIN5 protein content. Data are presented as mean ± SEM & were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-41576AF350
Alexa Fluor 405	NB110-41576AF405
Alexa Fluor 488	NB110-41576AF488
Alexa Fluor 532	NB110-41576AF532
Alexa Fluor 594	NB110-41576AF594
Alexa Fluor 647	NB110-41576AF647
Alexa Fluor 700	NB110-41576AF700
Alexa Fluor 750	NB110-41576AF750
Allophycocyanin	NB110-41576APC
Biotin	NB110-41576B
CoraFluor 1	NB110-41576CL1
DyLight 350	NB110-41576UV
DyLight 405	NB110-41576V
DyLight 488	NB110-41576G
DyLight 550	NB110-41576R
DyLight 594	NB110-41576DL594
DyLight 650	NB110-41576C
DyLight 680	NB110-41576FR
DyLight 755	NB110-41576IR
FITC	NB110-41576F
HRP	NB110-41576H
Janelia Fluor 525	NB110-41576JF525
Janelia Fluor 549	NB110-41576JF549
Janelia Fluor 646	NB110-41576JF646
Janelia Fluor 669	NB110-41576JF669
PE	NB110-41576PE
PerCP	NB110-41576PCP
mFluor Violet 450 SE	NB110-41576MFV450
mFluor Violet 500 SE	NB110-41576MFV500
mFluor Violet 610 SE	NB110-41576MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-41576SS

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NBP1-78159

LDLR Antibody (C7) - BSA Free

(1 Review)

(24 Publications)

0.1 ml

NBP1-78159

Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.

Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.

(A) Flowchart of the experiments used for testing the feasibility of the new system for enrichment of cells genome-edited at a single target locus. Four days after transfection with pCGsap1/LDLR, pgRNA#3, and pEGFP-N1, cells were treated with IB4SAP for a short period and cultured in normal medium for more than 10 days. The emerging colonies were propagated for molecular biological and cytochemical analyses; (B) cytochemical staining of clones LA-2 and -6 with AF594-IB4. Untransfected MPEFs were stained as positive control. Phase, photographs taken under light microscopy; AF594-IB4, photographs taken under UV illumination to detect AF594-IB4–derived red fluorescence. Bar = 30 μm; (C) electrophoretic pattern of polymerase chain reaction (PCR) products derived from the amplification of LA-1 to -7. Arrows indicate the size of PCR products for each gene (LDLR and GGTA1). M, 100 bp-ladder markers; (D) direct sequencing of PCR products from LA-2 (left panel) and LA-6 (right panel) using primers specific to LDLR or GGTA1 gene. Arrows above ideograms indicate the sites showing indels. In the bottom of each panel, sequencing results of inserts sub-cloned into TA cloning vector are shown. The numbers of clones examined are shown in parentheses. The translation initiation codon ATG is shown by boxes or in red. PAM is indicated by underlines. The deleted portion in the clones is shown by dotted lines; (E) immunocytochemistry of LA-6 and intact MPEFs (used as positive control) using anti-LDLR antibody. The parental MPEFs reactive to the second antibody (fluorescein-labeled anti-mouse IgG) alone are designated as negative control. All cells were counterstained with DAPI upon reaction with the second antibody. Bar = 30 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29207527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Po, Bv

Species Glossary

Reactivity: Hu, Rt, Po, Bv

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78159AF350
Alexa Fluor 405	NBP1-78159AF405
Alexa Fluor 488	NBP1-78159AF488
Alexa Fluor 532	NBP1-78159AF532
Alexa Fluor 594	NBP1-78159AF594
Alexa Fluor 647	NBP1-78159AF647
Alexa Fluor 700	NBP1-78159AF700
Alexa Fluor 750	NBP1-78159AF750
Allophycocyanin	NBP1-78159APC
Allophycocyanin/Cy7	NBP1-78159APCCY7
Biotin	NBP1-78159B
CoraFluor 1	NBP1-78159CL1
DyLight 350	NBP1-78159UV
DyLight 405	NBP1-78159V
DyLight 488	NBP1-78159G
DyLight 550	NBP1-78159R
DyLight 594	NBP1-78159DL594
DyLight 650	NBP1-78159C
DyLight 680	NBP1-78159FR
DyLight 755	NBP1-78159IR
FITC	NBP1-78159F
HRP	NBP1-78159H
Janelia Fluor 525	NBP1-78159JF525
Janelia Fluor 549	NBP1-78159JF549
Janelia Fluor 646	NBP1-78159JF646
Janelia Fluor 669	NBP1-78159JF669
PE	NBP1-78159PE
PE/Atto594	NBP1-78159PEATT594
PE/Cy5.5	NBP1-78159PECY55
PE/Cy7	NBP1-78159PECY7
PerCP	NBP1-78159PCP
mFluor Violet 450 SE	NBP1-78159MFV450
mFluor Violet 500 SE	NBP1-78159MFV500
mFluor Violet 610 SE	NBP1-78159MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78159SS
Azide and BSA Free	NBP2-80833

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NB100-654

HIF-1 alpha Antibody - BSA Free

(1 Review)

(19 Publications)

0.1 ml

NB100-654

<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-654] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody NB100-654 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - HIF1-alpha analysis using NB100-654. Image submitted from verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-654.

Simple Western: HIF-1 alpha Antibody [NB100-654] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Gel Super Shift Assays: HIF-1 alpha Antibody [NB100-654] - Mouse HIF-1 alpha+/+ and HIF-1 alpha+/- cells were exposed to hypoxia or 125 uM CoCl2 for 6 h, nuclear extracts were then prepared and EMSA carried out. HIF-1 isolated from hypoxia or CoCl2-treated cells bound to the wild-type probe (lanes #1-6) but not to the mutant probe (lanes #7-12). HIF-1/DNA complex was detected in hypoxia (H)-or CoCl2 (Co)-treated cells (lanes #2, 3, 5, 6) but not in control (C) cells (lanes #1, 4). More complex (darker band) was seen in hypoxia-or CoCl2-treated HIF-1 alpha+/+ cells (lanes #2, 3) than that in hypoxia-or CoCl2-treated HIF-1 alpha+/- cells (lanes #5, 6). Supershift assay showed that the HIF-1/DNA complex was shifted up in the presence of wild-type (wt) oligo probe and 4 ug HIF-1 alpha antiboby (lanes #13 & 14). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. (A) The cells were incubated in the presence or absence of 125 μM CoCl2 for 6 hr. HIF-1 alpha mRNA expression was determined by RT-PCR. Each bar represents the mean ± SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisk & number sign indicate significant difference compared to relevant controls (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). (B) Western blots using nuclear proteins show that both hypoxia (H) & CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha +/+ & HIF-1 alpha +/- cells. There was no HIF-1 alpha protein detected in control cells (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17474992), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-654AF350
Alexa Fluor 405	NB100-654AF405
Alexa Fluor 488	NB100-654AF488
Alexa Fluor 532	NB100-654AF532
Alexa Fluor 594	NB100-654AF594
Alexa Fluor 647	NB100-654AF647
Alexa Fluor 700	NB100-654AF700
Alexa Fluor 750	NB100-654AF750
Allophycocyanin	NB100-654APC
Biotin	NB100-654B
CoraFluor 1	NB100-654CL1
DyLight 350	NB100-654UV
DyLight 405	NB100-654V
DyLight 488	NB100-654G
DyLight 550	NB100-654R
DyLight 594	NB100-654DL594
DyLight 650	NB100-654C
DyLight 680	NB100-654FR
DyLight 755	NB100-654IR
FITC	NB100-654F
HRP	NB100-654H
Janelia Fluor 525	NB100-654JF525
Janelia Fluor 549	NB100-654JF549
Janelia Fluor 646	NB100-654JF646
Janelia Fluor 669	NB100-654JF669
PE	NB100-654PE
PerCP	NB100-654PCP
mFluor Violet 450 SE	NB100-654MFV450
mFluor Violet 500 SE	NB100-654MFV500
mFluor Violet 610 SE	NB100-654MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-654SS

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NB110-94158

MAT2A Antibody - BSA Free

(3 Reviews)

(19 Publications)

0.1 ml

NB110-94158

Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MAT2A Antibody (NB110-94158) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: MAT2A Antibody [NB110-94158] - Loss of PKM2 impairs methylation capacity, reduces DNA methylation and leads to the expression of endogenous retroviral elements. Western blot analysis of MAT2A, P53, P21 and PKM2 in control, P53KD, PKM2KD and PKM2KD/P53KD ECs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06406-8), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MAT2A Antibody [NB110-94158] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of MAT2A antibody (NB110-94158). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Western Blot: MAT2A Antibody [NB110-94158] - Total protein from HeLa and HepG2 cells and Human and Mouse Kidney was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-MAT2A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.

Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - MAT2A antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - Mouse embryonic stem cells (mESCs) were fixed with paraformaldehyde, permeabilized with Triton, blocked with 4% BSA and 1% normal goat serum, and incubated with 1:200 dilution of MAT2A antibody. Then, samples were stained with anti-rabbit Alexa Fluor 594 (1:1000) and NucBlue. ICC/IF image submitted by a verified customer review.

Simple Western: MAT2A Antibody [NB110-94158] - Human HepG2 cell lysate (0.2 ug/uL total protein). Antibody at 1:250. Simple Western image submitted by a verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Bv, Pm, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Ch

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-94158AF350
Alexa Fluor 405	NB110-94158AF405
Alexa Fluor 488	NB110-94158AF488
Alexa Fluor 532	NB110-94158AF532
Alexa Fluor 594	NB110-94158AF594
Alexa Fluor 647	NB110-94158AF647
Alexa Fluor 700	NB110-94158AF700
Alexa Fluor 750	NB110-94158AF750
Allophycocyanin	NB110-94158APC
Biotin	NB110-94158B
CoraFluor 1	NB110-94158CL1
DyLight 350	NB110-94158UV
DyLight 405	NB110-94158V
DyLight 488	NB110-94158G
DyLight 550	NB110-94158R
DyLight 594	NB110-94158DL594
DyLight 650	NB110-94158C
DyLight 680	NB110-94158FR
DyLight 755	NB110-94158IR
FITC	NB110-94158F
HRP	NB110-94158H
Janelia Fluor 525	NB110-94158JF525
Janelia Fluor 549	NB110-94158JF549
Janelia Fluor 646	NB110-94158JF646
Janelia Fluor 669	NB110-94158JF669
PE	NB110-94158PE
PerCP	NB110-94158PCP
mFluor Violet 450 SE	NB110-94158MFV450
mFluor Violet 500 SE	NB110-94158MFV500
mFluor Violet 610 SE	NB110-94158MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-94158SS

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NBP1-06709

LDLR Antibody - BSA Free

(1 Review)

(18 Publications)

0.1 ml

NBP1-06709

Simple Western: LDLR Antibody [NBP1-06709] - LDL R Antibody [NBP1-06709] - Simple Western lane view shows a specific band for LDL R in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: LDLR Antibody [NBP1-06709] - LDL R Antibody [NBP1-06709] - Western Blot on HepG2 whole cell lysate.

Immunocytochemistry/Immunofluorescence: LDL R Antibody [NBP1-06709] - LDL receptor antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: Rabbit Polyclonal LDLR Antibody [NBP1-06709] - Analysis of LDLR in bovine and canine corpus luetum. Image for a verified customer review.

Reactivity:

Hu, Mu, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-06709AF350
Alexa Fluor 405	NBP1-06709AF405
Alexa Fluor 488	NBP1-06709AF488
Alexa Fluor 532	NBP1-06709AF532
Alexa Fluor 594	NBP1-06709AF594
Alexa Fluor 647	NBP1-06709AF647
Alexa Fluor 700	NBP1-06709AF700
Alexa Fluor 750	NBP1-06709AF750
Allophycocyanin	NBP1-06709APC
Biotin	NBP1-06709B
CoraFluor 1	NBP1-06709CL1
DyLight 350	NBP1-06709UV
DyLight 405	NBP1-06709V
DyLight 488	NBP1-06709G
DyLight 550	NBP1-06709R
DyLight 594	NBP1-06709DL594
DyLight 650	NBP1-06709C
DyLight 680	NBP1-06709FR
DyLight 755	NBP1-06709IR
FITC	NBP1-06709F
HRP	NBP1-06709H
Janelia Fluor 525	NBP1-06709JF525
Janelia Fluor 549	NBP1-06709JF549
Janelia Fluor 646	NBP1-06709JF646
Janelia Fluor 669	NBP1-06709JF669
PE	NBP1-06709PE
PerCP	NBP1-06709PCP
mFluor Violet 450 SE	NBP1-06709MFV450
mFluor Violet 500 SE	NBP1-06709MFV500
mFluor Violet 610 SE	NBP1-06709MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-06709SS

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NBP2-46892

LC3B Antibody (1251A) - BSA Free

(3 Reviews)

(15 Publications)

0.1 mg

NBP2-46892

Western Blot: LC3B Antibody (1251A) [NBP2-46892] - The expression of LC3B in rat tissue. This image was submitted via customer Review.

<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - Jurkat cells were either untreated (A) or treated with 50uM chloroquine for 24 hours (B). An intracellular stain was performed with anti-LC3B (1251A) antibody [Catalog # NBP2-46892] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Effects of PDTC on LC3 on the hippocampus of rats harvested 12 h after CLP. Values of protein data are normalized to actin level and given in relative arbitrary units. LC3-II increased in CLP rats receiving vehicle or PDTC compared with those in sham-operated rats. PDTC + CLP rats showed an increase in LC3-II compared with vehicle-treated rats. Data were expressed as mean +/- SEM, n = 6/group. *P < 0.05 vs sham-operated group. # P < 0.05 vs 12 h or Veh + CLP group. PDTC pyrrolidine dithiocarbamate, LC-3 microtubule-associated protein light chain-3, CLP cecal ligation and puncture. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26067996) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with Chloroquine for 24 hours prior to fixation, permeabilization and incubation with anti- [Catalog # NBP2-46892] and anti tubulin (NB100-690) antibodies. Image enlargement shows the accumulation of LC3 (green) on autophagosomes in response to Chloroquine treatment. Tubulin staining is shown in red and DNA is counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: LC3B Antibody (1251A) [NBP2-46892] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.

Flow Cytometry: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells untreated or treated with 50 uM chloroquine for 24h. Image from the Alexa Fluor 405 version of this antibody.

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Analysis shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with Chloroquine. PVDF membrane was probed with 0.2 ug/mL rabbit anti-LC3B monoclonal Antibody (1251A) (Catalog # NBP2-46892, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody. A specific band was detected for LC3B at a molecular weight of approximately 15 kDa in CQ treated NIH-3T3 and PC12 cell lines.

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis using HeLa cells treated with (+) or without (-) Chloriquine (CQ). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa.

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of monoclonal anti-LC3B Antibody (Clone 1251D) [Catalog # NBP2-46892]. HeLa and Neuro2A cells were treated with or without 50 uM chloroquine for 24 hours as indicated. Whole cell protein was then separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 min and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2 ug/ml anti-LC3B Antibody in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the accumulation of LC3B II upon chloroquine treatment. Bands for LC3 were detected at a molecular weight of approximately 15 kDa in treated HeLA cells, and both treated and untreated Neuro2A cells.

Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti- [Catalog # NBP2-46892] and 1:500 anti-tubulin [Catalog # NB100-690] for 1 h at room temperature. LC3B reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).

Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - HeLa human cervical epithelial carcimoa cell line either treated with 50 uM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit anti-Human LC3B Alexa Fluor 647 conjugated monoclonal antiobdy (1251A) [Catalog # NBP2-46892AF647]. To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer Kit (FC012-NOV).

Immunoprecipitation: LC3B Antibody (1251A) [NBP2-46892] - Western blot analysis of LC3 immunoprecipitation. 10 ug of monoclonal anti-LC3B antibody (clone 1251A) [Catalog # NBP2-46892] was used to immunoprecipitate LC3 from 200 ug of total protein from HeLa cells treated with or without 50 uM chloroquine for 24 h. Antibody-protein was captured by magnetic protein A/G beads, washed and subjected to SDS-PAGE on a 4-15% gel. Protein was transferred to 0.2 um PVDF membrane and probed with 2 ug/ml anti-LC3B (NB100-2220) and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence. The detection of LC3 at a molecular weight of approximately 12 kDa and the antibody heavy chain (Ab Hc) at a molecular weight of approximately 50 kDa is indicated.

Western Blot: LC3B Antibody (1251A) - BSA Free [NBP2-46892] - Effects of PDTC on LC3 (a), Beclin1 (b), LAMP-1 (c), & Rab7 (d) on the hippocampus of rats harvested 12 h after CLP. Values of protein data are normalized to actin level & given in relative arbitrary units. LC3-II increased while Beclin1, Rab7, & LAMP-1 declined in CLP rats receiving vehicle or PDTC compared with those in sham-operated rats. PDTC + CLP rats showed an increase in LC3-II, Beclin1, Rab7, & LAMP-1 compared with vehicle-treated rats. Data were expressed as mean ± SEM, n = 6/group. *P < 0.05 vs sham-operated group. #P < 0.05 vs 12 h or Veh + CLP group. PDTC pyrrolidine dithiocarbamate, LC-3 microtubule-associated protein light chain-3, CLP cecal ligation & puncture Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0336-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LC3B Antibody (1251A) - BSA Free [NBP2-46892] - a LC3 in brains harvested 3, 6, 12, 24, & 48 h after CLP. b Immunofluorescence for LC3 in neurons after CLP injury. Values of protein data were related to signals obtained from actin protein & given as relative arbitrary units. LC3-II significantly increased in CLP rats at 6, 12, 24, & 48 h after surgery compared with sham-operated rats. Three-color staining for anti-LC3 antibody (green), NeuN (red), & DAPI (blue) showed that a staining pattern changes from largely diffuse to predominantly punctate & cytoplasmic in hippocampal neurons after CLP. Data were expressed as mean ± SEM, n = 6/group. *P < 0.05 vs sham-operated group. LC-3 microtubule-associated protein light chain-3, CLP cecal ligation & puncture, DAPI 4,6-diamidino-2-phenylindole Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0336-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, Ze

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-46892AF350
Alexa Fluor 405	NBP2-46892AF405
Alexa Fluor 488	NBP2-46892AF488
Alexa Fluor 532	NBP2-46892AF532
Alexa Fluor 594	NBP2-46892AF594
Alexa Fluor 647	NBP2-46892AF647
Alexa Fluor 700	NBP2-46892AF700
Alexa Fluor 750	NBP2-46892AF750
Allophycocyanin	NBP2-46892APC
Biotin	NBP2-46892B
CoraFluor 1	NBP2-46892CL1
DyLight 350	NBP2-46892UV
DyLight 405	NBP2-46892V
DyLight 488	NBP2-46892G
DyLight 550	NBP2-46892R
DyLight 594	NBP2-46892DL594
DyLight 650	NBP2-46892C
DyLight 680	NBP2-46892FR
DyLight 755	NBP2-46892IR
FITC	NBP2-46892F
HRP	NBP2-46892H
Janelia Fluor 525	NBP2-46892JF525
Janelia Fluor 549	NBP2-46892JF549
Janelia Fluor 646	NBP2-46892JF646
Janelia Fluor 669	NBP2-46892JF669
PE	NBP2-46892PE
PerCP	NBP2-46892PCP
mFluor Violet 450 SE	NBP2-46892MFV450
mFluor Violet 500 SE	NBP2-46892MFV500
mFluor Violet 610 SE	NBP2-46892MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-46892SS
Azide and BSA Free	NBP2-80829

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NB110-40764

Perilipin-3/TIP47 Antibody - BSA Free

(18 Publications)

0.1 ml

NB110-40764

Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle. Representative blots of PLIN3, ATGL, ARF1, ARFRP1 and loading control GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0091675), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).

Reactivity:

Hu, Mu, Po, Pm

Species Glossary

Reactivity: Hu, Mu, Po, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-40764AF350
Alexa Fluor 405	NB110-40764AF405
Alexa Fluor 488	NB110-40764AF488
Alexa Fluor 532	NB110-40764AF532
Alexa Fluor 594	NB110-40764AF594
Alexa Fluor 647	NB110-40764AF647
Alexa Fluor 700	NB110-40764AF700
Alexa Fluor 750	NB110-40764AF750
Allophycocyanin	NB110-40764APC
Biotin	NB110-40764B
CoraFluor 1	NB110-40764CL1
DyLight 350	NB110-40764UV
DyLight 405	NB110-40764V
DyLight 488	NB110-40764G
DyLight 550	NB110-40764R
DyLight 594	NB110-40764DL594
DyLight 650	NB110-40764C
DyLight 680	NB110-40764FR
DyLight 755	NB110-40764IR
FITC	NB110-40764F
HRP	NB110-40764H
Janelia Fluor 525	NB110-40764JF525
Janelia Fluor 549	NB110-40764JF549
Janelia Fluor 646	NB110-40764JF646
Janelia Fluor 669	NB110-40764JF669
PE	NB110-40764PE
PerCP	NB110-40764PCP
mFluor Violet 450 SE	NB110-40764MFV450
mFluor Violet 500 SE	NB110-40764MFV500
mFluor Violet 610 SE	NB110-40764MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-40764SS

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NB300-591

LAMP-2/CD107b Antibody

(1 Review)

(17 Publications)

100 uL

NB300-591

Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues stained with anti-LAMP-2/CD107b.

Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 25ug of NRK cell lysates using anti-LAMP-2/CD107b.

$Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Validation of subcellular fractionation and immunocytochemistry. RLC proteins are present at ER microsomes. Lysates from 7 DIV cortical neurons were subjected to differential centrifugation to isolate nuclear pellet (P1), postnuclear supernatant (PNS), mitochondrial pellet (P2), and ER microsomal pellet (P3), as depicted in the graphic on the right. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embr.201744853) licensed under a CC-BY license.$

Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 10 ug of HeLa (left panel), NIH/3T3 (middle panel) and NRK (right panel) whole cell lysates using anti-LAMP-2/CD107b.

Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b (green) in NIH/3T3 cells.

Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human tonsils were stained with anti-LAMP-2/CD107b.

Exposure of microglia to cART resulted in blockade of autophagosome–lysosome fusion. (A) rPMs were seeded into a 12-well plate followed by tandem fluorescent-tagged MAP1LC3B plasmid. Next, cells were exposed to cART (5 µM each of TDF, FTC, & DTG) for an additional 24 h & observed by confocal imaging. The results showed that cART exposure significantly increased the formation of autophagosomes (yellow puncta). (B) Representative bar graph showing the number of autophagosome (yellow puncta) per cell. (C) Representative bar graph showing the number of autolysosome (red puncta) per cell. (D) rPMs were seeded into 12-well plates followed with cART exposure for 24 h. Cells were then double immunostained with MAP1LC3B & LAMP2 antibody & observed by immunofluorescent microscopy. (E,F) Representative bar graphs showing cART-mediated decreased LAMP2 puncta & decreased colocalization of MAP1LC3B & LAMP2. BAF—autophagosome fusion inhibitor, & rapamycin (RAP—autophagy inducer) were used as controls for autophagy flux. Data is from three independent experiments & is expressed as means ± SEM & were analyzed using one-way ANOVA. *, p < 0.05 vs. control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Exposure of rat primary microglial cells (rPMs) to combined antiretroviral therapy (cART) cocktail resulted in impaired lysosomal function. rPMs were seeded into six-well plates & treated with cART (5 µM each of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), & dolutegravir (DTG)) for the indicated time periods. (A,B) Exposure of microglia to cART resulted in a significant decrease in expression of lysosomal-associated membrane protein 2 (LAMP2) at 6 to 24 h post-treatment. (C) Representative bar graph showing cART-mediated significantly increased lysosomal membrane permeabilization (LMP) (24 h). (D,E) Microglia exposed to cART demonstrated a significant decrease in levels of mature cathepsin D (mCTSD) at 24 h post-treatment. (F) Representative bar graph showing exposure of cART significantly reduced the CTSD activity in rPMs (24 h). (G) Representative bar graph showing cART-mediated increased lysosomal pH in rPMs. (H,I) Acridine orange staining showing increased green color & reduced red color in cART-treated rPMs. H2O2 was used as a positive control for lysosome damage. Data is from three independent experiments. Actin beta (ACTB) served as a protein loading control for western blots. Data are expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; N.S., non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LAMP-2/CD107b Antibody [NB300-591] - HSPA overexpression abrogated cART-mediated impairment of lysosomal function. Control rPMs & heat shock protein family A (HSPA) overexpressing rPMs were seeded into six-well plates subjected to various treatments for 24 h. Protein homogenates were prepared for the detection of the indicated molecules. (A,B) Representative western blots showing overexpressing HSPA in rPMs reversed cART-mediated downregulation of LAMP2 & mCTSD expression levels. (C) Representative bar graph showing overexpression of HSPA in rPMs protected lysosomal pH. (D,E) Representative bar graphs showing HSPA protected LMP (D), & CTSD activity (E) in cART-treated rPMs. For all western blots, ACTB served as a protein loading control. Data is from three independent experiments & is expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; #, p < 0.05 vs. cART. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-125

PER2 Antibody - BSA Free

(3 Reviews)

(13 Publications)

0.1 ml

NB100-125

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Deletion of IL-1 beta signaling ameliorates expression of hepatic circadian transcription factors following DSS-PN.8–10 week old IL1KO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. Values are Mean+/- SEM, N = at least 3/condition. *p<0.05. **p<0.01, ***P<0.001, ****P<0.0001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Genetic inhibition of TNF alpha signaling normalizes expression of hepatic circadian transcription factors following DSS-PN.8–10 week old TNFRKO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. N = 4 per condition. Values are Mean+/- SEM. *p<0.05. **p<0.01, ***p<0.001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-125AF350
Alexa Fluor 405	NB100-125AF405
Alexa Fluor 488	NB100-125AF488
Alexa Fluor 532	NB100-125AF532
Alexa Fluor 594	NB100-125AF594
Alexa Fluor 647	NB100-125AF647
Alexa Fluor 700	NB100-125AF700
Alexa Fluor 750	NB100-125AF750
Allophycocyanin	NB100-125APC
Biotin	NB100-125B
CoraFluor 1	NB100-125CL1
DyLight 350	NB100-125UV
DyLight 405	NB100-125V
DyLight 488	NB100-125G
DyLight 550	NB100-125R
DyLight 594	NB100-125DL594
DyLight 650	NB100-125C
DyLight 680	NB100-125FR
DyLight 755	NB100-125IR
FITC	NB100-125F
HRP	NB100-125H
Janelia Fluor 525	NB100-125JF525
Janelia Fluor 549	NB100-125JF549
Janelia Fluor 646	NB100-125JF646
Janelia Fluor 669	NB100-125JF669
PE	NB100-125PE
PerCP	NB100-125PCP
mFluor Violet 450 SE	NB100-125MFV450
mFluor Violet 500 SE	NB100-125MFV500
mFluor Violet 610 SE	NB100-125MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-125SS

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NB100-2068

ABCA1 Antibody (HJ1) - BSA Free

(17 Publications)

0.1 ml

NB100-2068

Flow Cytometry: ABCA1 Antibody (HJ1) [NB100-2068] - Staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ABCA1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2068AF350
Alexa Fluor 405	NB100-2068AF405
Alexa Fluor 488	NB100-2068AF488
Alexa Fluor 532	NB100-2068AF532
Alexa Fluor 594	NB100-2068AF594
Alexa Fluor 647	NB100-2068AF647
Alexa Fluor 700	NB100-2068AF700
Alexa Fluor 750	NB100-2068AF750
Allophycocyanin	NB100-2068APC
Allophycocyanin/Cy7	NB100-2068APCCY7
Biotin	NB100-2068B
CoraFluor 1	NB100-2068CL1
DyLight 350	NB100-2068UV
DyLight 405	NB100-2068V
DyLight 488	NB100-2068G
DyLight 550	NB100-2068R
DyLight 594	NB100-2068DL594
DyLight 650	NB100-2068C
DyLight 680	NB100-2068FR
DyLight 755	NB100-2068IR
FITC	NB100-2068F
HRP	NB100-2068H
Janelia Fluor 525	NB100-2068JF525
Janelia Fluor 549	NB100-2068JF549
Janelia Fluor 646	NB100-2068JF646
Janelia Fluor 669	NB100-2068JF669
PE	NB100-2068PE
PE/Atto594	NB100-2068PEATT594
PE/Cy5.5	NB100-2068PECY55
PE/Cy7	NB100-2068PECY7
PerCP	NB100-2068PCP
mFluor Violet 450 SE	NB100-2068MFV450
mFluor Violet 500 SE	NB100-2068MFV500
mFluor Violet 610 SE	NB100-2068MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2068SS
Azide and BSA Free	NBP2-80554

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Fatty Acid Synthase/FASN Antibody - BSA Free

GADD153/CHOP Antibody - BSA Free

Abhd5 Antibody - BSA Free

LDLR Antibody (C7) - BSA Free

HIF-1 alpha Antibody - BSA Free

MAT2A Antibody - BSA Free

LDLR Antibody - BSA Free

LC3B Antibody (1251A) - BSA Free

Perilipin-3/TIP47 Antibody - BSA Free

LAMP-2/CD107b Antibody

PER2 Antibody - BSA Free

ABCA1 Antibody (HJ1) - BSA Free