MAT2A Antibody

Images

 
Western Blot: MAT2A Antibody [NB110-94158] - Loss of PKM2 impairs methylation capacity, reduces DNA methylation and leads to the expression of endogenous retroviral elements. Western blot analysis of MAT2A, P53, P21 and ...read more
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NB110-94158] - Mouse embryonic stem cells (mESCs) were fixed with paraformaldehyde, permeabilized with Triton, blocked with 4% BSA and 1% normal goat serum, and ...read more
Immunohistochemistry-Paraffin: MAT2A Antibody [NB110-94158] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of MAT2A antibody (NB110-94158). The staining was developed using HRP labeled ...read more
Western Blot: MAT2A Antibody [NB110-94158] - Total protein from HeLa and HepG2 cells and Human and Mouse Kidney was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NB110-94158] - MAT2A antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Simple Western: MAT2A Antibody [NB110-94158] - Human HepG2 cell lysate (0.2 ug/uL total protein). Antibody at 1:250. Simple Western image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, ChSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MAT2A Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human MAT2A (within residues 1-100). [Swiss-Prot# P31153]
Localization
Cytoplasm
Predicted Species
Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This MAT2A antibody is useful for Western blot, where a band is seen at ~43 kDa, and Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HepG2 cells. Use in immunoprecipitation reported in scientific literature (PMID: 25294683).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MAT2A Overexpression Lysate
Control Peptide
MAT2A Peptide (NB110-94158PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB110-94158 in the following applications:

Publications
Read Publications using
NB110-94158 in the following applications:

  • IP
    1 publication
  • WB
    7 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29133573).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAT2A Antibody

  • AdoMet synthase 2
  • AMS2
  • EC 2.5.1.6
  • MAT 2
  • MAT2A
  • MATA2
  • MATA2SAMS2adoMet synthetase 2
  • MATII
  • MAT-II
  • Methionine adenosyltransferase 2
  • Methionine adenosyltransferase II
  • methionine adenosyltransferase II, alpha
  • S-adenosylmethionine synthase isoform type-2
  • S-adenosylmethionine synthetase isoform type-2
  • SAMS2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-94158
Species: Hu, Mu, Rt, Po, Bv, Pm, Ch
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for MAT2A Antibody (NB110-94158)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
Porcine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB110-94158 Applications Species
Secker KA, Bloechl B, Keppeler H et al. MAT2A as Key Regulator and Therapeutic Target in MLLr Leukemogenesis Cancers (Basel) May 24 2020 [PMID: 32456310] (WB, Human) WB Human
Wheeler MA, Clark IC, Tjon EC et al. MAFG-driven astrocytes promote CNS inflammation Nature Feb 1 2020 [PMID: 32051591] (WB, Mouse) WB Mouse
Roy DG, Chen J, Mamane V et al. Methionine Metabolism Shapes T Helper Cell Responses through Regulation of Epigenetic Reprogramming Cell Metab. Feb 4 2020 [PMID: 32023446] (Mouse) Mouse
Stone OA, El-Brolosy M, Wilhelm k et al. Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells. Nat Commun. Oct 9 2018 [PMID: 30301887] (WB, Human) WB Human
Zhong Q, Liu Z, Lin ZR et al. The RARS-MAD1L1 Fusion Gene Induces Cancer Stem Cell-like Properties and Therapeutic Resistance in Nasopharyngeal Carcinoma Clin. Cancer Res. 2017 Nov 13 [PMID: 29133573] (WB, Porcine) WB Porcine
Tomasi ML, Cossu C, Spissu Y et al. S-adenosylmethionine and methylthioadenosine inhibit cancer metastasis by targeting microRNA 34a/b-methionine adenosyltransferase 2A/2B axis Oncotarget. 2017 Oct 03 [PMID: 29108270] (WB, Mouse) WB Mouse
Scotton C, Bovolenta M, Schwartz E et al. Deep RNA profiling identified clock and molecular clock genes as pathophysiological signatures in collagen VI myopathy. J. Cell. Sci. 2016 Mar 04 [PMID: 26945058] (WB, Human, Mouse) WB Human, Mouse
Ramani K, Donoyan S, Tomasi Ml, Park S. Role of Methionine Adenosyltransferase Alpha2 and B Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation. J. Cell. Physiol. 2014 Oct 07 [PMID: 25294683] (IP, Human) IP Human
Ramani K, Tomasi ML. Transcriptional regulation of methionine adenosyltransferase 2A by peroxisome proliferator-activated receptors in rat hepatic stellate cells. Hepatology. 2012 Jan 23. [PMID: 22271545] (WB, Mouse) WB Mouse

Reviews for MAT2A Antibody (NB110-94158) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse and Mus musculus.

Reviews using NB110-94158:
Filter by Applications
Simple Western
(1)
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse and Mus musculus
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western MAT2A NB110-94158
Enlarge
4
reviewed by:
Anonymous
Simple Western Human 02/03/2020
View

Summary

ApplicationSimple Western
Sample TestedHepG2 CELLS
SpeciesHuman
LotB-1

Comments

CommentsMatériel: 1x25caps 12-230kDa
échantillon : 0.2µg/µL
anticorps : 1/250
Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
Immunocytochemistry MAT2A NB110-94158
Enlarge
5
reviewed by:
Anonymous
ICC Mouse and Mus musculus 02/18/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedJ1 embryonic stem cells,Mouse Embryonic Stem Cells
SpeciesMouse and Mus musculus
LotE-2

Comments

CommentsAntibody behaved as expected in IF (different conditions gave different levels of fluorescence that matched qPCR and Western blot results very well).
Western Blot MAT2A NB110-94158
Enlarge
4
reviewed by:
Steve Smith
WB Human 11/26/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate prepared from HEK 293 cells
SpeciesHuman

Blocking

Blocking DetailsPVDF was blocked with 5% milk in PBS buffer

Primary Anitbody

Dilution RatioPrimary antibody diluted 1000 times in PBS buffer

Secondary Antibody

Secondary DescriptionBioRad goat anti-rabbit HRP
Secondary Manufacturer Cat#170-5046
Secondary Concentration20000 times diluted

Details

Detection NotesDeveloped using BioRad WesternC Chemiluminescence Kit, 30 sec exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAT2A Antibody (NB110-94158). (Showing 1 - 1 of 1 FAQs).

  1. We received an enquiry about the following antibody: NB110-94158. Since the protein sequence between MAT1A and MAT2A shares high homology, the customer would like to know how specific NB110-94158 is for MAT2A. I checked the alignment between the antibody immunogen and the MAT1A protein and this is actually high at 76%. Therefore, it is likely that the antibody would cross-react with the MAT1A protein especially as it is a polyclonal antibody. I would appreciate if you can provide any available data about cross-reactivity testing on the NB110-94158 specificity for MAT2A. Any information you can provide is highly appreciated.
    • The immunogen for NB110-94158 shares under 45% similarity with MAT1A. It is extremely unlikely that this antibody would cross-react with MAT1A, given the large difference in homology. Customer feedback suggests that this antibody is absolutely specific for MAT2A.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-94158AF350
Alexa Fluor 405 NB110-94158AF405
Alexa Fluor 488 NB110-94158AF488
Alexa Fluor 532 NB110-94158AF532
Alexa Fluor 594 NB110-94158AF594
Alexa Fluor 647 NB110-94158AF647
Alexa Fluor 700 NB110-94158AF700
Alexa Fluor 750 NB110-94158AF750
Biotin NB110-94158B
DyLight 350 NB110-94158UV
DyLight 405 NB110-94158V
DyLight 488 NB110-94158G
DyLight 550 NB110-94158R
DyLight 594 NB110-94158DL594
DyLight 650 NB110-94158C
DyLight 680 NB110-94158FR
DyLight 755 NB110-94158IR
FITC NB110-94158F
HRP NB110-94158H
Janelia Fluor 549 NB110-94158JF549
Janelia Fluor 646 NB110-94158JF646

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Bioinformatics Tool for MAT2A Antibody (NB110-94158)

Discover related pathways, diseases and genes to MAT2A Antibody (NB110-94158). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MAT2A Antibody (NB110-94158)

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Pathways for MAT2A Antibody (NB110-94158)

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PTMs for MAT2A Antibody (NB110-94158)

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Research Areas for MAT2A Antibody (NB110-94158)

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Blogs on MAT2A.

MAT2A - A Protien Coding Gene
The enzyme S-adenosylmethionine synthetase, or MAT, catalyzes the formation of S- adenosylmethionine (AdoMet or SAMe) from methionine and ATP. AdoMet is the principal biological source of synthesized methyl, and is found in all cells but most prominen...  Read full blog post.

MAT2a, MAT2b, HIF-1 alpha: Roles in Liver Cancer and DNA methylation
Methionine Adenosyltransferase II alpha, also known as MAT2a, is a catalytic subunit of methionine adenosyltransferase (MAT) and essential enzyme for the catalysis of the principle biological methyl donor, S-adenosylmethionine (SAM) from methionine an...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
02/03/2020
Application: Simple Western
Species: Human

Anonymous
02/18/2019
Application: ICC
Species: Mouse and Mus musculus

Steve Smith
11/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAT2A