MAT2A Antibody

Images

 
Western Blot: MAT2A Antibody [NB110-94158] - Total protein from HeLa and HepG2 cells and Human and Mouse Kidney was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NB110-94158] - MAT2A antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, Po, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MAT2A Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human MAT2A (within residues 1-100). [Swiss-Prot# P31153]
Localization
Cytoplasm
Predicted Species
Porcine (94%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:2000
Application Notes
This MAT2A antibody is useful for Western blot, where a band is seen at ~43 kDa, and Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MAT2A Lysate (NBL1-12910)
Control Peptide
MAT2A Peptide (NB110-94158PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB110-94158 in the following applications:

Publications
Read Publications using
NB110-94158 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Monkey. 86% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAT2A Antibody

  • AdoMet synthase 2
  • AMS2
  • EC 2.5.1.6
  • MAT 2
  • MATA2SAMS2adoMet synthetase 2
  • MATII
  • MAT-II
  • Methionine adenosyltransferase 2
  • Methionine adenosyltransferase II
  • methionine adenosyltransferase II, alpha
  • S-adenosylmethionine synthase isoform type-2
  • S-adenosylmethionine synthetase isoform type-2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAT2A Antibody (NB110-94158)(3)

Review for MAT2A Antibody (NB110-94158) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-94158:
Filter by Applications
WB
(1)
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Human
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Images Ratings Applications Species Date Details
Western Blot MAT2A NB110-94158
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reviewed by:
Steve Smith
WB Human 11/26/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate prepared from HEK 293 cells
SpeciesHuman

Blocking

Blocking DetailsPVDF was blocked with 5% milk in PBS buffer

Primary Anitbody

Dilution RatioPrimary antibody diluted 1000 times in PBS buffer

Secondary Antibody

Secondary DescriptionBioRad goat anti-rabbit HRP
Secondary Manufacturer Cat#170-5046
Secondary Concentration20000 times diluted

Details

Detection NotesDeveloped using BioRad WesternC Chemiluminescence Kit, 30 sec exposure

Product General Protocols

View specific protocols for MAT2A Antibody (NB110-94158): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAT2A Antibody (NB110-94158). (Showing 1 - 1 of 1 FAQs).

  1. We received an enquiry about the following antibody: NB110-94158. Since the protein sequence between MAT1A and MAT2A shares high homology, the customer would like to know how specific NB110-94158 is for MAT2A. I checked the alignment between the antibody immunogen and the MAT1A protein and this is actually high at 76%. Therefore, it is likely that the antibody would cross-react with the MAT1A protein especially as it is a polyclonal antibody. I would appreciate if you can provide any available data about cross-reactivity testing on the NB110-94158 specificity for MAT2A. Any information you can provide is highly appreciated.
    • The immunogen for NB110-94158 shares under 45% similarity with MAT1A. It is extremely unlikely that this antibody would cross-react with MAT1A, given the large difference in homology. Customer feedback suggests that this antibody is absolutely specific for MAT2A.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-94158AF405
Alexa Fluor 488 Labeled NB110-94158AF488
Alexa Fluor 647 Labeled NB110-94158AF647
Alexa Fluor 700 Labeled NB110-94158AF700
Biotin Labeled NB110-94158B
DyLight 405 Labeled NB110-94158V
DyLight 488 Labeled NB110-94158G
DyLight 550 Labeled NB110-94158R
DyLight 650 Labeled NB110-94158C
FITC Labeled NB110-94158F
HRP Labeled NB110-94158H

Additional MAT2A Products

Bioinformatics Tool for MAT2A Antibody (NB110-94158)

Discover related pathways, diseases and genes to MAT2A Antibody (NB110-94158). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MAT2A Antibody (NB110-94158)

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Pathways for MAT2A Antibody (NB110-94158)

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PTMs for MAT2A Antibody (NB110-94158)

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Research Areas for MAT2A Antibody (NB110-94158)

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Blogs on MAT2A.

MAT2A - A Protien Coding Gene
The enzyme S-adenosylmethionine synthetase, or MAT, catalyzes the formation of S- adenosylmethionine (AdoMet or SAMe) from methionine and ATP. AdoMet is the principal biological source of synthesized methyl, and is found in all cells but most prominen...  Read full blog post.

MAT2a, MAT2b, HIF-1 alpha: Roles in Liver Cancer and DNA methylation
Methionine Adenosyltransferase II alpha, also known as MAT2a, is a catalytic subunit of methionine adenosyltransferase (MAT) and essential enzyme for the catalysis of the principle biological methyl donor, S-adenosylmethionine (SAM) from methionine an...  Read full blog post.

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Recent Reviews

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Steve Smith
11/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAT2A