Western Blot: MAT2A Antibody [NB110-94158] - Loss of PKM2 impairs methylation capacity, reduces DNA methylation and leads to the expression of endogenous retroviral elements. Western blot analysis of MAT2A, P53, P21 and ...read more
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NB110-94158] - Mouse embryonic stem cells (mESCs) were fixed with paraformaldehyde, permeabilized with Triton, blocked with 4% BSA and 1% normal goat serum, and ...read more
Immunohistochemistry-Paraffin: MAT2A Antibody [NB110-94158] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of MAT2A antibody (NB110-94158). The staining was developed using HRP labeled ...read more
Western Blot: MAT2A Antibody [NB110-94158] - Total protein from HeLa and HepG2 cells and Human and Mouse Kidney was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NB110-94158] - MAT2A antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Simple Western: MAT2A Antibody [NB110-94158] - Human HepG2 cell lysate (0.2 ug/uL total protein). Antibody at 1:250. Simple Western image submitted by a verified customer review.
This MAT2A antibody is useful for Western blot, where a band is seen at ~43 kDa, and Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HepG2 cells. Use in immunoprecipitation reported in scientific literature (PMID: 25294683).
43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Porcine reactivity reported in scientific literature (PMID: 29133573).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for MAT2A Antibody
AdoMet synthase 2
MATA2SAMS2adoMet synthetase 2
Methionine adenosyltransferase 2
Methionine adenosyltransferase II
methionine adenosyltransferase II, alpha
S-adenosylmethionine synthase isoform type-2
S-adenosylmethionine synthetase isoform type-2
MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MAT2A Antibody (NB110-94158). (Showing 1 - 1 of 1 FAQs).
We received an enquiry about the following antibody: NB110-94158. Since the protein sequence between MAT1A and MAT2A shares high homology, the customer would like to know how specific NB110-94158 is for MAT2A. I checked the alignment between the antibody immunogen and the MAT1A protein and this is actually high at 76%. Therefore, it is likely that the antibody would cross-react with the MAT1A protein especially as it is a polyclonal antibody. I would appreciate if you can provide any available data about cross-reactivity testing on the NB110-94158 specificity for MAT2A. Any information you can provide is highly appreciated.
The immunogen for NB110-94158 shares under 45% similarity with MAT1A. It is extremely unlikely that this antibody would cross-react with MAT1A, given the large difference in homology. Customer feedback suggests that this antibody is absolutely specific for MAT2A.
MAT2A - A Protien Coding Gene The enzyme S-adenosylmethionine synthetase, or MAT, catalyzes the formation of S- adenosylmethionine (AdoMet or SAMe) from methionine and ATP. AdoMet is the principal biological source of synthesized methyl, and is found in all cells but most prominen... Read full blog post.
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