| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-139SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74678SS | |||
| Azide and BSA Free | NBP2-80876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70526 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37387-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB19553-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-62091SS | |||
| Azide and BSA Free | NBP2-80878 | |||
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0005.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Apolipoprotein A5 (1G5G9) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunocytochemistry-Immunofluorescence-NB400-139-img0006.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB400-139-img0004.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0003.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Detection of Apo A5 in HepG2 cell lysate with NB400-139.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0002.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser605-Antibody-10A8-Western-Blot-NB110-74678-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.")
![Immunocytochemistry/Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01] - Analysis of monoclonal antibody to CDS1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Immunocytochemistry-Immunofluorescence-H00001040-M01-img0005.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 monoclonal antibody (M01), clone 2D10. Analysis of CDS1 expression in human liver.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0007.jpg)
![ELISA: CDS1 Antibody (2D10) [H00001040-M01] - Detection limit for recombinant GST tagged CDS1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-ELISA-H00001040-M01-img0008.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-31020241548365.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415483622.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415482211.jpg)
![Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Western-Blot-NBP2-01800-img0026.jpg "Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunocytochemistry-Immunofluorescence-NBP2-01800-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0024.jpg)
![Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2B6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Flow-Cytometry-NBP2-01800-img0014.jpg)
![Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2B6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2B6.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Western-Blot-NBP2-01800-img0025.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0019.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0020.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0021.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0022.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Immunohistochemistry-Paraffin-NBP2-01800-img0023.jpg)
![Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-3D5-Flow-Cytometry-NBP2-01800-img0013.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp2-27561_goat-polyclonal-enpp-1-antibody-imgenex-img-3895-12920231134472.jpg "Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.")
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.](http://images.novusbio.com/fullsize/nbp2-27561_goat-polyclonal-enpp-1-antibody-imgenex-img-3895-12920231134473.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NB100-1471-img0005.jpg "Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Western-Blot-NBP1-06985-img0001.jpg "Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-06985] - Human ARPE-19 cells. Antibody at 1:100 overnight. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06985-img0003.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Immunohistochemistry-Paraffin-NBP1-06985-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0004.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.")
![Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Flow-Cytometry-NBP2-37387-img0005.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0003.jpg)
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-ELISA-NBP2-37387-img0001.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: CD36 Antibody (877302) [MAB19553] - Analysis of human platelets tissue lysate with 1 ug/ml of hCD36 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hCD36 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Western-Blot-MAB19553-img0002.jpg "Western Blot: CD36 Antibody (877302) [MAB19553] - Analysis of human platelets tissue lysate with 1 ug/ml of hCD36 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hCD36 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: CD36 Antibody (877302) [MAB19553] - CD36 was detected in immersion fixed U937 cell using CD36 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Immunocytochemistry-MAB19553-img0001.jpg)
![Immunohistochemistry: CD36 Antibody (877302) [MAB19553] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using CD36 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cardiomyocytes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Immunohistochemistry-MAB19553-img0003.jpg)
![Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate](http://images.novusbio.com/fullsize/NHE8-Antibody-7A11-Western-Blot-NB110-62091-img0003.jpg "Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate")
