Ferritin Heavy Chain Antibody

Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-06985] - Human ARPE-19 cells. Antibody at 1:100 overnight. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Ferritin Heavy Chain Antibody Summary

• Immunogen: Peptide with sequence C-DKHTLGDSDNES corresponding to C-Terminus according to NP_002023.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FTH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
• Application Notes: This Ferritin Heavy Chain Antibody is validated for ICC/IF from a verified customer review.
• Control:

  Ferritin Heavy Chain Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ferritin Heavy Chain Antibody

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferritin Heavy Chain Antibody (NBP1-06985)(3)

Publications using NBP1-06985

• Ashok A, chaudhary S, Wise A et al. Release of Iron-Loaded Ferritin in Sodium Iodate-Induced Model of Age Related Macular Degeneration: An In-Vitro and In-Vivo Study Antioxidants (Basel) 2021-08-27 [PMID: 34439501]
• Bossoni L, Grand Moursel L, Bulk M et al. Human-brain ferritin studied by muon spin rotation: a pilot study J Phys Condens Matter 2017-10-18 [PMID: 28872048] (WB, Human)
• Sammarco MC, Ditch S, Banerjee A, Grabczyk E. Ferritin L and H subunits are differentially regulated on a post-transcriptional level. J Biol Chem 2008-02-22 [PMID: 18160403]

Reviews for Ferritin Heavy Chain Antibody (NBP1-06985) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Ajay Ashok ICC Human 01/27/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: ARPE-19 cells
• Species: Human
• Lot: G2E2706B

Comments

• Comments: Use at a concentration of 1:100 overnight

reviewed by: Ajay Ashok WB Human 06/16/2017

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Human

Bioinformatics

• Gene Symbol: FTH1
• Entrez:
  • 2495(Human)
• Uniprot:
  • NP_002023.2(Human)