Ferritin Heavy Chain Antibody

Images

 
Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-06985] - Human ARPE-19 cells. Antibody at 1:100 overnight. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Ferritin Heavy Chain Antibody Summary

Immunogen
Peptide with sequence C-DKHTLGDSDNES corresponding to C-Terminus according to NP_002023.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FTH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
Application Notes
This Ferritin Heavy Chain Antibody is validated for ICC/IF from a verified customer review.
Control
Ferritin Heavy Chain Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-06985 in the following applications:

Publications
Read Publications using
NBP1-06985 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ferritin Heavy Chain Antibody

  • apoferritin
  • Cell proliferation-inducing gene 15 protein
  • Ferritin H subunit
  • ferritin heavy chain
  • ferritin, heavy polypeptide 1
  • FHC
  • FTHEC 1.16.3.1
  • FTHL6MGC104426
  • PIG15
  • placenta immunoregulatory factor
  • PLIF
  • proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ferritin Heavy Chain Antibody (NBP1-06985)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
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Filter By Species
Human
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-06985 Applications Species
Ashok A, chaudhary S, Wise A et al. Release of Iron-Loaded Ferritin in Sodium Iodate-Induced Model of Age Related Macular Degeneration: An In-Vitro and In-Vivo Study Antioxidants Jun 5 2021 (ICC/IF, Human) ICC/IF Human
Bossoni L, Grand Moursel L, Bulk M et al. Human-brain ferritin studied by muon spin rotation: a pilot study J Phys Condens Matter 2017 Oct 18 [PMID: 28872048] (WB, Human) WB Human
Sammarco MC, Ditch S, Banerjee A, Grabczyk E. Ferritin L and H subunits are differentially regulated on a post-transcriptional level. J Biol Chem 2008 Feb 22 [PMID: 18160403]

Reviews for Ferritin Heavy Chain Antibody (NBP1-06985) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-06985:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Ferritin Heavy Chain NBP1-06985
Enlarge
5
reviewed by:
Verified Customer
ICC Human 01/27/2021
View

Summary

ApplicationImmunocytochemistry
Sample TestedARPE-19 cells
SpeciesHuman
LotG2E2706B

Comments

CommentsUse at a concentration of 1:100 overnight
Western Blot Ferritin Heavy Chain NBP1-06985
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/16/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ferritin Heavy Chain Antibody (NBP1-06985) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
01/27/2021
Application: ICC
Species: Human

Verified Customer
06/16/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol FTH1
Entrez
Uniprot