| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30328SS | |||
| Azide and BSA Free | NBP2-80712 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87695-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
![Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Western-Blot-NBP1-30328-img0004.jpg "Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.")
![Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Immunohistochemistry-NBP1-30328-img0005.jpg)
![Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Lane view shows a specific band for EGLN1/PHD2 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Simple-Western-NBP1-30328-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0013.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0006.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody [NB300-612] - analysis of LXR alpha was done on HeLa. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 2.5% BSA for 30 minutes at room temperature. Cells were labeled with LXR alpha Rabbit Polyclonal Antibody (PA1330, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Flow-Cytometry-NB300-612-img0012.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody (2ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples was electrophoresed using 4-12 % Bis-Tris gel, Electrophoresis System and Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Blot 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31944-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NBP1-31944-img0009.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunohistochemistry-Paraffin-NBP1-31944-img0004.jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-202202482557..jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020242011458.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020241957213.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-1610202419414513.jpg)
![Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-141120251833591.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-141120251839311.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0005.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of rat hippocampus stained with NBP1-92683 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Western blot of rat olfactory epithelium (OE) and frontal cortex (FC). NBP1-92683 stains a band at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Mixed neuron-glial cultures stained with Adenylate Cyclase 3 antibody (NBP1-92683, red) and Alpha Fodrin antibody (NBP1-92689, green). Note the strong and clean staining of neuronal cilia. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0003.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human liver and pancreas tissues. Corresponding FABP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human liver and pancreas tissues. Corresponding FABP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP1/L-FABP Antibody [NBP1-87695] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Western-Blot-NBP1-87695-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87695-img0010.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0020.jpg)
![Western Blot: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Western-Blot-NBP1-87695-img0006.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human pancreas shows no positivity in exocrine glandular cells and islets of Langerhans as expected.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0015.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0016.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0019.jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183346.jpg "Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918336.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183311.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918331.jpg)
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183335.jpg)
![Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NB100-57086-img0006.jpg "Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB100-57086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57086-img0008.jpg)
![Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunohistochemistry-Paraffin-NB100-57086-img0005.jpg)
![Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Flow-Cytometry-NB100-57086-img0007.jpg)
![Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proprotein-Convertase-9-PCSK9-Antibody-Western-Blot-NB300-959-img0005.jpg "Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-5ug-ml-staining-of-paraffin-embedded-Human-Liver-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254023.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-Negative-Control-showing-staining-of-paraffin-embedded-Human-Liver-with-no-primary-antibody--2932023925404.jpg)
![Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-Frozen-NBP1-30032-img0006.jpg "Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0001.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg)
![Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression & western blot analyses of ABCA4 protein levels in the canine retina.(A) Relative ABCA4 mRNA expression levels by quantitative RT-PCR in three different regions in three dogs with different genotypes (ABCA4+/+, ABCA4+/-, & ABCA4-/-), normalized to GAPDH expression. (B) Western blot analyses of ABCA4 (above), GAPDH (middle), & RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202415371970.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-3102024161606.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616064.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616097.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202416175238.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0013.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of LOVO cells labeling Lactate Dehydrogenase with Rabbit anti-Lactate Dehydrogenase A/LDHA antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lactate Dehydrogenase antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0014.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lactate Dehydrogenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0015.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Flow-Cytometry-NBP2-67483-img0001.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0011.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase A/LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0003.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0004.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0005.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0006.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0007.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0008.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0009.jpg)