| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-39474SS | |||
| Azide and BSA Free | NBP2-80562 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55376SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19388SS | |||
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP monoclonal antibody (M01), clone 2E2-D3 Analysis of TARDBP expression in A-431.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Western-Blot-H00023435-M01-img0025.jpg "Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP monoclonal antibody (M01), clone 2E2-D3 Analysis of TARDBP expression in A-431.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP in rat tissue samples. Image courtsey of product review by Daniel Pirici.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Immunohistochemistry-Paraffin-H00023435-M01-img0011.jpg)
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - PDI co-precipitates with TDP-43/TARDBP and is increased in mutant TDP-43 transgenic mouse spinal cords. Co-immunoprecipitation of wildtype and mutant Q331K TDP-43-mCherry from Neuro2a cell lysates using an anti-PDI antibody followed by TDP-43 immunoblot. Control immunoprecipitations were performed using untransfected (Untrans) or mCherry expressing cell lysates, buffer only with coprecipitating antibodies or TDP-43 Q331K cell lysates using an irrelevant, isotype-matched control antibody (IgG). Input control (2%) shows expression of mCherry-TDP43 in wildtype and Q331K cell lysates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081170) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Western-Blot-H00023435-M01-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Immunocytochemistry-Immunofluorescence-H00023435-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Immunohistochemistry-Paraffin-H00023435-M01-img0013.jpg)
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.Lane 1: TARDBP transfected lysate(44.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Western-Blot-H00023435-M01-img0026.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Immunohistochemistry-Paraffin-H00023435-M01-img0021.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi ( Cat # H00023435-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody (M01), clone 2E2-D3 (Cat # H00023435-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-Western-Blot-H00023435-M01-img0023.jpg)
![ELISA: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Detection limit for recombinant GST tagged TARDBP is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-2E2-D3-ELISA-H00023435-M01-img0027.jpg)
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - PDI co-precipitates with TDP-43 & is increased in mutant TDP-43 transgenic mouse spinal cords.(A) Co-immunoprecipitation of wildtype & mutant Q331K TDP-43-mCherry from Neuro2a cell lysates using an anti-PDI antibody followed by TDP-43 immunoblot. Control immunoprecipitations performed using untransfected (Untrans) or mCherry expressing cell lysates, buffer only with coprecipitating antibodies or TDP-43 Q331K cell lysates using an irrelevant, isotype-matched control antibody (IgG). Input control (2%) shows expression of mCherry-TDP43 in wildtype & Q331K cell lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081170), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023435-m01_mouse-monoclonal-tdp-43-tardbp-antibody-2e2-d3-310202415284521.jpg)
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - CLIP shows that TDP-43 directly interacts with translational target mRNAs. (A) Schematic diagram of the CLIP experiment. (B) Immunoblot showing specific IP of TDP-43 from MN1 cells under CLIP conditions. A TDP-43 band is specifically observed in the input & TDP-43 IP lanes from both untreated & UV-treated cells, but not in the IgG or anti-RBP X control lanes. RBP X is an IP of a different RNA-binding protein, which serves as an additional specificity control here. (C and D) Plots showing percentage of input mRNA in the TDP-43 IP for the mRNAs indicated in three separate CLIP assays from MN1 cells. Performing CLIP from UV-treated cells leads to significant recovery & enrichment relative to Gapdh for all mRNAs examined (C). In the absence of UV treatment, much less mRNA is recovered & no significant enrichment relative to Gapdh mRNA is observed. Note that the Y-axis scale in D is 1000× smaller than in C, indicating strong crosslinking dependence of mRNA co-immunoprecipitation (n = 3, s.e.m. error bars, P-values indicated in the plot, unpaired two-tailed t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30357366), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023435-m01_mouse-monoclonal-tdp-43-tardbp-antibody-2e2-d3-310202415483647.jpg)
![Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - SINE compounds partially rescue motor deficits in an animal model of ALS/FTD. (a) Schematic timeline for in vivo experiments. (b) Western blot demonstrating the expression of human TDP43 within the brains of AAV9-injected animals, which we have previously shown is largely restricted to neuronal cell types (Jackson et al.59). Full-length blots appear in Fig. S1. (c) The hang test measures the time it takes for animals to fall from grid-like scaffold when it is lifted & tilted. A single representative animal administered AAV9-TDP43 is shown; note the inability of the hind limbs to clasp the wire mesh. (d) TDP43 expression impaired motor performance on the hang test at 3 weeks, an effect that could be mitigated by 7.5 mg kg-1 KPT-350. (e) Rotarod testing at 4 weeks of age showed prominent deficits associated with TDP43 expression that were resistant to treatment with SINE compounds. n, number of animals per condition. ns, not significant, mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Bonferroni correction. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-22858-w), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023435-m01_mouse-monoclonal-tdp-43-tardbp-antibody-2e2-d3-310202415482260.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1beta and TNF-alpha in the SDH of CYP-induced cystitis. Western blots showing the expression of BDNF was further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean +/- SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NB100-98682-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1beta and TNF-alpha in the SDH of CYP-induced cystitis. Western blots showing the expression of BDNF was further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean +/- SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC and EA treatment on the expression of mBDNF and NT4 in neurons. Photomicrographs for mBDNF/NeuN or NT4/NeuN double-positive cells in the striatum and hippocampus (n = 5). The number of mBDNF/NeuN double-positive cells in the SVZ + striatum and hippocampus was significantly higher in the EA-treated MCAO group and the combined mBMSC + EA-treated MCAO group, compared to the number in the other groups. The number of NT4/NeuN double-positive cells in the hippocampus was significantly higher in the EA-treated MCAO group than in the vehicle-treated MCAO group. #p < 0.05 and ##p < 0.01 versus control group; *p < 0.05, **p < 0.01, and ***p < 0.001 versus vehicle-treated MCAO group; &&p < 0.01 versus mBMSC-treated MCAO group. Scale bar = 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-018-20481-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NB100-98682-img0007.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NB100-98682-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0003.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0005.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415371951.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415175223.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Antagonized TrkB could restrain the activation of astrocytes & microglia & supress the p38/JNK pathway to alleviate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. Western blots showed that the overexpression of a BDNF, b TrkB, cp-TrkB, d Iba1, e GFAP, fp-p38, gp-JNK, h TNF-alpha , & i IL-1 beta were downregulated in comparison to the CYP + DMSO group after ANA-12 treatment. All data were calculated as mean ± SEM (n = 5 per group). **p < 0.01, ***p < 0.001 vs. control group. ##p < 0.01, ###p < 0.001 vs. CYP + ANA-12 group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415291934.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further & promoted activation of astrocytes & microglia, & enhanced the p38/JNK pathway to aggravate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. a BDNF treated every other day after CYP injection could further lower the mechanical withdrawal threshold & suppress the retrieval of mechanical threshold when compared with the CYP + PBS group. After the exogenous BDNF injection, Western blots showing the expression of b BDNF, c TrkB, dp-TrkB, e Iba1, f GFAP, gp-p38, hp-JNK, i TNF-alpha , & j IL-1 beta were all further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean ± SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284921.jpg)
![Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC & EA treatment on the expression of trophic factors. (A) Photomicrographs & (B‒D) histograms for mBDNF, NT4, & VEGF expression (n = 4–5). mBDNF expression was markedly increased by treatment with mBMSC and/or EA compared to vehicle treatment. mBDNF expression levels were higher in the EA-treated MCAO group than in the mBMSC-treated MCAO group. NT4 expression in the SVZ & hippocampus was significantly higher in the combined mBMSC & EA treatment group than in the other groups. VEGF expression in the hippocampus was markedly higher in the mBMSC treatment group than in the vehicle-treated MCAO group. #p < 0.05, ##p < 0.01, & ###p < 0.001 versus control group; *p < 0.05, **p < 0.01, & ***p < 0.001 versus vehicle-treated MCAO group; &p < 0.05, &&p < 0.01, & &&&p < 0.001 versus mBMSC-treated MCAO group; Scale bar = 1 mm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29391466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284912.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - The expression of BDNF-TrkB signaling in the SDH of CYP-induced cystitis. a Changes of the mechanical threshold in CYP-induced cystitis model. Compared to that in the control group, the mechanical threshold of the cystitis group decreased significantly after the CYP injection & remained low until day 17, & the minimum threshold value was reached on day 12. The expression of b BDNF, c TrkB, & dp-TrkB were evaluated by western blots. Compared to the control group, they were upregulated on days 8, 12, & 17. e Immunofluorescence double staining assay of BDNF & p-TrkB in the SDH. BDNF & p-TrkB (red), NeuN, GFAP, & OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. & TrkB receptors expressed in neurons, microglia, & astrocytes. The white dotted lines in picture “BDNF/NeuN” showed the laminate of the SDH according to Rexed & Steiner. Scale bar = 100 μm. All data were calculated as mean ± SEM (n = 5 per group). *p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284910.jpg)
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Western-Blot-NBP1-39474-img0003.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0002.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0008.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0009.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0011.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0012.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0013.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin (APC).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0014.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0015.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0016.jpg)
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0012.jpg "Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with TDP-43/TARDBP Antibody conjugated to Alexa Fluor 488 (NB110-55376AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-TARDBP at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0011.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody [NB110-55376] - Analysis of FFPE human placenta using TDP-43 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunohistochemistry-Paraffin-NB110-55376-img0010.jpg)
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in HeLa WCE.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0004.jpg)
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in MCF-7 cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Analysis using the DyLight 550 conjugate of NB110-55376. Staining of Human iPS derived neurons, fixed in 4% formaldehyde solution. NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Blue dots in TDP staining is Hoechst.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Subcellular distribution of TDP-43 and CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0013.jpg)
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-46750-img0002.jpg "Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)")
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0008.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0003.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0005.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0007.jpg)
![Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-Western-Blot-NBP1-19388-img0004.jpg "Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19388-img0003.jpg)
![Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis of the EPO receptor in human placenta.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-19388-img0002.jpg)
![Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view shows a specific band for EPO Receptor in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-Simple-Western-NBP1-19388-img0005.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Proteins were extracted from control (Ctrl) and stretched (Stretch) 158N cells and western blot was performed for myelin protein PLP (26 kDa) following 20% of strain. Beta-Actin was used as a loading control. Results represent the mean +/- SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6505516/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0003.jpg "Western Blot: Myelin PLP Antibody [NB100-74503] - Proteins were extracted from control (Ctrl) and stretched (Stretch) 158N cells and western blot was performed for myelin protein PLP (26 kDa) following 20% of strain. Beta-Actin was used as a loading control. Results represent the mean +/- SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6505516/) licensed under a CC-BY license.")
![Western Blot: Myelin PLP Antibody [NB100-74503] - Figure 1 shows a Western blot of PLP in adult mouse brain samples using NB100-74503. Figure 2 shows an immunocytochemical staining of PLP in mouse brain thick section using NB100-74503. Staining (m) is limited to myelin tracts and oligodendrocytes.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0001.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Analysis of adult mouse brain samples.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0002.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% & 30%. Total RNA was extracted & RT-qPCR was performed in control (Ctrl) & stretched (Stretch) cells for myelin genes Mag (a, d), Cnp (b, e), & Plp (c, f, g, i) after strain of 20% (a, b, c, g) & 30% (d, e, f, i). Results represent the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05; **p < 0.01. (h, j) Proteins were extracted from control (Ctrl) & stretched (Stretch) 158N cells & western blot was performed for myelin protein PLP (26 kDa) following 20% (h) & 30% (j) of strain. beta -Actin was used as a loading control. Results represent the mean ± SEM of three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-310202415392571.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% & 30%. Total RNA was extracted & RT-qPCR was performed in control (Ctrl) & stretched (Stretch) cells for myelin genes Mag (a, d), Cnp (b, e), & Plp (c, f, g, i) after strain of 20% (a, b, c, g) & 30% (d, e, f, i). Results represent the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05; **p < 0.01. (h, j) Proteins were extracted from control (Ctrl) & stretched (Stretch) 158N cells & western blot was performed for myelin protein PLP (26 kDa) following 20% (h) & 30% (j) of strain. beta -Actin was used as a loading control. Results represent the mean ± SEM of three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-310202415334915.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-3102024168216.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-31020241555233.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-3102024165743.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0009.jpg "Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.")
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Ventral horn region of mouse spinal cord. Concentration 1:200](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0004.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0005.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0002.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates, Antibody Dilution: 1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0006.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Fetal Liver lysates, Antibody Dilution: 0.5 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0007.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Sample Tissue: Human ACHN Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0008.jpg)
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Rhesus macaque spinal cord. Concentration1:300.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0003.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Simvastatin treatment increases BDNF expression in primary cortical neurons of AS mice. (A) Primary cultured cortical neurons prepared from wild type & AS embryos were treated with 5 μM simvastatin at DIV14 for 12 h. Neurons were then fixed & processed for double immunofluorescence staining using Ube3a & BDNF antibodies. Representative images were shown. About 12 immunostained neurons in each group were checked for BDNF expression, fluorescence intensity in each cell was quantified & compared. Scale bar, 50 μm. (B) Immunoblot analysis of matured BDNF levels in simvastatin treated primary cortical neurons as described above. (C) Band intensity of the mature BDNF was quantified & normalized to Tuj1 (BDNF/Tuj1). Values are mean ± SD; n = 3. The ‘a’ point P < 0.05 compared to vehicle treated wild type group & “b” denote P < 0.05 with respect to vehicle treated AS group (one way ANOVA with Holm–Sidak post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31849603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59304_rabbit-polyclonal-bdnf-antibody-310202416182644.jpg)
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)