| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-235SS | |||
| Azide and BSA Free | NBP2-80597 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65541SS | |||
| Azide and BSA Free | NBP2-80849 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25196SS | |||
| Azide and BSA Free | NBP2-80634 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78295SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24965SS | |||
| Azide and BSA Free | NBP2-80633 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76739-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47840-0.1mg | |||
| IHC-Prediluted | NBP2-48335 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48028 | |||
| Azide and BSA Free | NBP2-34637-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44740 | |||
| Azide and BSA Free | NBP2-34653-0.1mg | |||
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0028.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunohistochemistry-Paraffin-NB600-235-img0025.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-BSA-Free-Flow-Cytometry-NB600-235-img0032.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0027.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0011.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0013.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0015.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0024.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0029.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0030.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - The effect of IL-21 & CD40L exposure on MEC1 & MEC2 cells.Expression of EBNA-2 & LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) & LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 & upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 & Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes & 5×105 were loaded in both untreated & IL-21 treated MEC1 & MEC2 lanes. Note low expression of EBNA-2 & high expression of LMP-1 after IL-21 treatment & induction of Blimp-1 after IL-21 treatment. (C) Activity of the W & C promoters that regulate EBNA-2 expression & LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp & Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 & LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 & LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-235_mouse-monoclonal-blimp1-prdm1-antibody-3h2-e8-31020241616096.jpg)
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, and pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells and plasma. Representative merged images above show co-localization of OX-6 (Green) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 expression in intestinal villi. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0017.jpg "Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, and pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells and plasma. Representative merged images above show co-localization of OX-6 (Green) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 expression in intestinal villi. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.")
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0011.jpg)
![Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-Paraffin-NB100-65541-img0014.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0005.jpg)
![Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis of fluorescent intensity revealed significant protection and retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells and plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6). Overall, the combination of cord blood cells and plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production and immune cell activation in the 6-OHDA rat model at all time points tested. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0019.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0012.jpg)
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0002.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044625.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044643.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, & pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells & plasma. This therapy also resulted in a significant decrease in TNF expression in this animal model. Quantitative analyses of the estimated OX-6 expression & TNF are displayed in the graphs above. Representative merged images above show co-localization of OX-6 (Green) or TNF (Red) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 & TNF expression in intestinal villi. Scale bar = 100 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415532025.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of TH-positive dopaminergic neurons, immune cell activation, & pro-inflammatory cytokine production. Tyrosine hydroxylase (TH) staining (top row) was used to investigate the potential effect of combined cord blood cell with plasma injection on dopaminergic neuron populations in the substantia nigra pars compacta (SNpc). Analysis of fluorescent intensity revealed significant protection & retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells & plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6) & pro-inflammatory cytokine production (TNF). Overall, the combination of cord blood cells & plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production & immune cell activation in the 6-OHDA rat model at all time points tested. Photomicrographs correspond to representative SN in coronal sections immune-labeled with TH, OX-6 or TNF antibody. Scale bar = 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415523917.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - CD19 antibody (CB19) was tested in Non-Hodgkin's lymphoma cells at 1:200 dilution. Green: Alexa Flour 488. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Immunocytochemistry-Immunofluorescence-NBP2-25196-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - CD19 antibody (CB19) was tested in Non-Hodgkin's lymphoma cells at 1:200 dilution. Green: Alexa Flour 488. Image from verified customer review.")
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Immunocytochemistry-Immunofluorescence-NBP2-25196-img0002.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0001.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0007.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0008.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0009.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0010.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0011.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0012.jpg)
![Western Blot: BTK [p Tyr223] Antibody [NBP1-78295] - WB analysis of BTKpY223 on Ramos whole cell lysate with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Western-Blot-NBP1-78295-img0004.jpg "Western Blot: BTK [p Tyr223] Antibody [NBP1-78295] - WB analysis of BTKpY223 on Ramos whole cell lysate with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.")
![Immunohistochemistry: BTK [p Tyr223] Antibody [NBP1-78295] - IHC analysis of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-NBP1-78295-img0002.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0011.jpg "Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.")
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in mouse splenocytes, 25 ug/10^6 cells. Anti-rat PE secondary and cell surface staining kit were used in this test.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0001.jpg)
![Flow (Cell Surface): CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents anti-CD19 APC.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cell-Surface-NBP2-24965-img0006.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 PE this antibody and either 1 ug of isotype control antibody (left) or 1 ug of anti-CD19 APC (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0007.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the PE conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 FITC this antibody and either 1 ug of isotype control antibody (left, ) or 1 ug of anti-CD19 PE (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0010.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0009.jpg "Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.")
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Raji cells with MS4A1/CD20 antibody (MEM-97) NBP1-44634AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0001.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Daudi cells with MS4A1/CD20 (MEM-97) antibody NBP1-44634PE (blue) along with a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0002.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibod](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0006.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Flow Cytometry: MS4A1/CD20 Antibody (MEM-97) [Alexa Fluor 647] [NBP1-44634AF647] - A surface stain was performed on human peripheral blood lymphocytes with MS4A1/CD20 (MEM-97) antibody NBP1-44634AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0007.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Ramos cells with CD20 antibody (MEM-97) NBP1-44634PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0008.jpg)
![Immunoprecipitation: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97)](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Immunoprecipitation-NBP1-44634-img0005.jpg)
![Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-NBP1-76739-img0006.jpg "Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)). White bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76739-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in Western Blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0008.jpg)
![Western Blot: IL-21R Antibody [NBP1-76739] - Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 ug /ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-21R Antibody [NBP1-76739] - Rat lung with IL-21 receptor antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-Paraffin-NBP1-76739-img0001.jpg)
![Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - Treatment of avian xenografts with IL-21 upregulates Blimp-1 expression. (A) The three-dimensional growth of BxPC-3 cells transplanted on the CAM of fertilized chicken eggs & resected tumors (three replicates) from vehicle control & IL-21 were photographed at day 17 of embryonic development. Upper row for vehicle control; lower row for IL-21-treated (150 ng/mL) tumors. (B) HE & IL-21R (brown) IHC staining of vehicle control & IL-21-treated (150 ng/mL) resected xenograft tumors. (C) Comparison & quantification of Blimp-1 (nuclei: red) expression of vehicle control & IL-21-treated (150 ng/mL) xenograft tumors. **** P Green: IL-21 Receptor Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-271120242011556.gif)
![Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-2711202419241320.jpg)
![Immunocytochemistry/Immunofluorescence: CD20 Antibody (IGEL/773) [NBP2-44745] - Immunofluorescence staining of Raji cells using CD20 Antibody (IGEL/773) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Immunocytochemistry-Immunofluorescence-NBP2-44745-img0006.jpg "Immunocytochemistry/Immunofluorescence: CD20 Antibody (IGEL/773) [NBP2-44745] - Immunofluorescence staining of Raji cells using CD20 Antibody (IGEL/773) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) [NBP2-44745] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (IGEL/773).](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44745-img0007.jpg)
![Flow Cytometry: CD20 Antibody (IGEL/773) [NBP2-44745] - Flow Cytometric Analysis of Raji cells. CD20 Antibody (IGEL/773) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Flow-Cytometry-NBP2-44745-img0005.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) [NBP2-44745] - Human Tonsil stained with CD20 Monoclonal Antibody (IGEL/773)](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44745-img0002.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) [NBP2-44745] - Human Lymphoma stained with CD20 Monoclonal Antibody (IGEL/773)](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44745-img0001.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) [NBP2-44745] - Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (C3e/1308). Image from the DyLight 594 version of this antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44745-img0004.jpg)
![Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Western-Blot-NB100-64992-img0003.jpg "Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was incubated with VisUBlock™ Mouse on Mouse Blocking Reagent (Catalog # VB001) and then subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Immunohistochemistry-Paraffin-NB100-64992-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Flow-Cytometry-NB100-64992-img0001.jpg)
![Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Western-Blot-NBP2-44738-img0005.jpg "Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (HM47/A9) [NBP2-44738] - Immunofluorescence Analysis of Raji cells labeling CD79a with CD79A Antibody (HM47/A9). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-44738-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0004.jpg)
![Flow Cytometry: CD79A Antibody (HM47/A9) [NBP2-44738] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Flow-Cytometry-NBP2-44738-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human tonsil stained with CD79a antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0001.jpg)
![Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Western-Blot-NBP2-34330-img0005.jpg "Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-34330-img0004.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0001.jpg)
![Flow Cytometry: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Flow-Cytometry-NBP2-34330-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0002.jpg)