| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56698SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24861 | |||
| BSA Free | NB100-56720SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27216 | |||
| Azide and BSA Free | NBP2-25199SS | |||
| Azide and BSA Free | NBP2-27216-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27369SS | |||
| Azide and BSA Free | NBP2-80862 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-24867 | |||
| BSA Free | NBP2-25186SS | |||
| Low Endotoxin, Azide and BSA Free | NBP2-24867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76337-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-245SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27363 | |||
| BSA Free | NBP2-27362SS | |||
| Azide and BSA Free | NBP2-27363-0.5mg | |||
| Azide and BSA Free | NBP2-27363-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-26585 | |||
| BSA Free | NBP2-25191SS | |||
| Azide and BSA Free | NBP2-26585-1mg | |||
| Azide and BSA Free | NBP2-26585-25ug | |||
![Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MyD88 Antibody [NB100-56698] - Raw264.7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MyD88 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56698-img0008.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0005.jpg)
![Western Blot: MyD88 Antibody [NB100-56698] - Analysis of MyD88 in human spleen cell lysate using 0.5 ug/ml of NB100-56698.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0003.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0004.jpg)
![Western Blot: MyD88 Antibody - BSA Free [NB100-56698] - TLR & other LPS-response elements in LPS-stimulated HSCs.(A) Microarray data show time-dependent changes in the indicated transcripts. For clarity control gene expression at 1 & 24h is offset to 0.5h & 24.5h respectively. (B) A representative Western blot (left pane) & densitometric analysis (right panel) of the indicated molecules at 24h following stimulation with 10 ng/ml LPS. (C) qPCR analysis of the indicated molecules with p values showing statistical differences. The values (B,C) shown are form 3 separate determinations from different batches of HSCs. Statistical significance was derived from student’s t-test using Microsoft-excel program. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0082159), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56698_rabbit-polyclonal-myd88-antibody-310202415371913.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Frozen: TLR3 Antibody [NB100-56571] - Frozen mouse eye tissue sections stained with TLR3, a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) marker. Bottom panel: Tissue stained with isotype control (no TLR3 Ab), RPE and DAPI. Data courtesy of Kleinman et al.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-Frozen-NB100-56571-img0002.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Analysis using the FITC conjugate of NB100-56571. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0008.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Analysis of TLR3 in mouse spleen tissue lysate using NB100-56571 at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0005.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Pig lung poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of IC ventilation. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-NB100-56571-img0006.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TLR3 Antibody [NB100-56571] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. Panel shows the knockdown effects of siRNAs. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Knockdown-Validated-NB100-56571-img0010.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415523938.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024165731.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415535158.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - TLR3‐knockout (TLR3‐KO) attenuated cardiac autophagy induced by MI. The infarct & remote tissues were separately sampled from the left ventricle after 4 weeks of MI. Accordingly, anterior & posterior tissues of the left ventricle were sampled from sham hearts as controls. (A) Representative Western blot images & quantitative analyses of autophagy markers. n = 4–8/group. Quantitative data are fold changes of WT‐sham. aP < 0.05, AP < 0.01 versus respective WT‐sham; bP < 0.05, BP < 0.01 versus respective WT‐MI. (B) Representative electron microphotographs of ultrathin sections of resin‐embedded heart biopsies. Arrows indicate autophagic vacuoles. (C) Lysates of infarct tissue were immunoprecipitated (IP), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG served as a control. Green arrows indicate non‐specific bands. Representative images were taken from four independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415525214.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - A TLR3 agonist polyinosinic‐polycytidylic acid (poly(I:C)) induced autophagy in cultured cardiomyocytes through a TRIF‐dependent pathway. (A) Poly(I:C) increased autophagy markers in cultured H9c2 rat ventricular cells. (B) Poly(I:C) stimulated autophagosome formation but did not affect autophagic flux. Primary cultured neonatal rat ventricular myocytes (NRVMs) were transfected with a tandem mRFP‐GFP‐LC3 adenovirus for 24 hrs, followed by treatment with poly(I:C) (100 μg/ml, 4 hrs). Autophagosomes & autolysosomes were, respectively, visualized as yellow‐ & red‐only punctas under a confocal microscope. (C) An autophagic flux inhibitor chloroquine (CQ) induced accumulations of LC3‐II & p62/SQSTM1 proteins in H9c2 myocytes receiving poly(I:C) (100 μg/ml, 4 hrs). CQ was applied at 10 μM, immediately prior to poly(I:C). (D) Effects of indicated siRNA on poly(I:C)‐induced changes in autophagy markers in NRVMs. All the siRNAs were transfected at 50 nM for 48 hrs, & poly(I:C) was added at 100 μg/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. The upper panel shows the knockdown effects of siRNAs, & the lower panel shows representative Western blot images (presented from four independent experiments) & densitometry quantitative data (normalized into ‘fold of vehicle group’). All quantitative data are expressed as means ± S.D. aP < 0.05, AP < 0.01 versus vehicle; bP < 0.05, BP < 0.01 versus poly(I:C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024168394.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-Western-Blot-NB100-56720-img0005.jpg)
![Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-Immunohistochemistry-Paraffin-NB100-56720-img0003.jpg)
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0010.jpg "In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.")
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0012.jpg)
![IKK gamma Inhibitor Peptide Set [NBP2-26504] - The IKK complex consists of IKKalpha + IKKbeta+ NEMO. NEMO (IKKgamma/NF-kB Essential MOdulator) is a scaffold protein required for the kinase activity of IKKalpha + IKKbeta. NBD (NEMO Binding Domain) inhibits formation of the IKK complex (IKKalpha + IKKbeta+ NEMO) by binding to NEMO and blocking IKK complex formation. In this regard, NBD is considered to be an 'IKKalpha and IKKBeta decoy' Blocking IKK complex formation suppresses downstream events in the NF-kB signaling pathway that rely on the IKK complex including phosphorylation of IkBalpha, and subsequence IkBalpha ubiquination, degradation and release of NF-kB. Hence, NF-kB is retained in the cytoplasm in its inactive state.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-NBP2-26504-img0014.jpg)
![Functional (Inhibition): IKK gamma Inhibitor Peptide Set [NBP2-26504] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-26504) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-26504-img0016.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0007.jpg "Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.")
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0004.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0009.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0010.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.](http://images.novusbio.com/fullsize/nbp2-25199_mouse-monoclonal-cd4-antibody-rpa-t4-imgenex-img-5916a-25ug-17720241044654.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0005.jpg "Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 antibody in MCF-7 cells using an isotype control (top) and MyD88 Antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-27369-img0002.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0012.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0001.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - Intracellular analysis of MyD88 antibody in Jurkat cells using 0.5 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody. This antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0003.jpg)
![Flow (Intracellular): MyD88 Antibody (4D6) [NBP2-27369] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-27369. Staining of MyD88 antibody in Jurkat cells using 10 ul (0.5 ug) of MyD88 antibody. Green represents isotype control (20108); red represents anti-MyD88 antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Intracellular-NBP2-27369-img0006.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 (4D6) antibody NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0007.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 [4D6] Antibody NBP2-27369AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0008.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0009.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on U-937 cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0010.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 Antibody (4D6) NBP2-27369F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0011.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0009.jpg "Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 700 conjugate of NBP2-24867. Staining of human PBMC. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0008.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Azide Free version of NBP2-25186. CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0005.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjugate) isotype control (blue). Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0013.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of PerCP conjugate of NBP2-25186. A surface stain was performed on Jurkat cells with CD3 (OKT3) antibody NBP2-24867PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0004.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue). Image using the Low Endotoxin and Azide Free form of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0011.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0013.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.")
![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0012.jpg)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A antibody at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420414565.gif)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17/IL-17A with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120241928814.jpg)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A antibody at 5 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420312943.gif)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17/IL-17A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202419293859.gif)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420171759.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17/IL-17A with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120242042535.gif)
![Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NB600-242-img0010.jpg "Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry: FoxP3 Antibody [NB600-242] - (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-242-img0008.jpg)
![Immunohistochemistry: FoxP3 Antibody [NB600-242] - (8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NB600-242-img0012.jpg)
![Flow Cytometry: FoxP3 Antibody [NB600-242] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB600-242-img0009.jpg)
![Immunofluorescence: FoxP3 Antibody [NB600-242] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-242-img0011.jpg)
![Control: FoxP3 Antibody [NB600-242] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Control-NB600-242-img0013.jpg)
![Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse stained with FoxP3 and CD4.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-245-img0013.jpg "Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse stained with FoxP3 and CD4.")
![Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse incubated only with CD4 and no Foxp3.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-245-img0012.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-245] - Fox P3 was detected in immersion fixed paraffin-embedded sections of human tonsil using rabbit anti-human antibody (Catalog # NB600-245) at 1:100 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (Catalog # VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB600-245-img0011.jpg)
![Western Blot: FOXP3 Antibody [NB600-245] - Detection of human FOXP3 using NB 600-245. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector](http://images.novusbio.com/fullsize/FOXP3-Antibody-Western-Blot-NB600-245-img0009.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD8A and FOXP3 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB600-245-img0014.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD4 and FOXP3 antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB600-245-img0015.jpg)
![Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.](http://images.novusbio.com/fullsize/EBI3-Antibody-15k8D10-Tissue-Culture-Substratum-NBP2-27362-img0001.jpg "Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.")
![Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/EBI3-Antibody-15k8D10-Immunohistochemistry-Paraffin-NBP2-27362-img0002.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) [NBP2-25191] - CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Clone GK1.5) at 1 ug/mL overnight at 4 degrees C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Immunohistochemistry-Frozen-NBP2-25191-img0005.jpg "Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) [NBP2-25191] - CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Clone GK1.5) at 1 ug/mL overnight at 4 degrees C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes.")
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the PE conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody ; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0008.jpg)
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0006.jpg)
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0007.jpg)