| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6405SS | |||
| Azide and BSA Free | NBP2-80848 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-64775-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33148 | |||
| IHC-Prediluted | NBP2-44386 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46603SS | |||
| Azide and BSA Free | NBP2-80846 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46603SS | |||
| BSA Free | NBP2-46603 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-65956-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50419-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47672-0.1mg | |||
| IHC-Prediluted | NBP2-48136 | |||
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P ≤0.01). (B) MHCII revealed a fainter basic expression emphasizing the nucleus and was found significantly increased after 0.5 ng/mL IL-17 stimulation (**P ≤ 0.01). (C) TAPII was detected in the nucleus and cytoplasm. We detected a dose-dependent tendency but no significantly increased expression after IL-17 stimulation. For MHCI, TAPII and MHCII and the concentrations applied, analysis of variance between the independent experiments revealed no significant difference. DAPI, 4′, 6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24678820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85960_rabbit-polyclonal-sfxn2-antibody-western-blot-13220261739815.jpg "Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P ≤0.01). (B) MHCII revealed a fainter basic expression emphasizing the nucleus and was found significantly increased after 0.5 ng/mL IL-17 stimulation (**P ≤ 0.01). (C) TAPII was detected in the nucleus and cytoplasm. We detected a dose-dependent tendency but no significantly increased expression after IL-17 stimulation. For MHCI, TAPII and MHCII and the concentrations applied, analysis of variance between the independent experiments revealed no significant difference. DAPI, 4′, 6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24678820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Immunohistochemistry-Frozen-NB100-64775-img0015.jpg "Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.")
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0013.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0002.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0004.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0006.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0007.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0008.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0009.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0010.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0014.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A19-P-Immunohistochemistry-Paraffin-NBP1-30151-img0012.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A19-P-Immunohistochemistry-Paraffin-NBP1-30151-img0011.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0011.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0007.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0005.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0006.jpg)
![Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Frozen-NBP2-15197-img0010.jpg)
![Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Western-Blot-NBP2-46603-img0005.jpg "Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0003.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-BSA-Free-Flow-Cytometry-NBP2-46603-img0007.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells. The tumor stroma cells did not display any Melan-A/MART-1 expression signal.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0002.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0004.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0006.jpg)
![Western Blot: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Western-Blot-NBP2-80846-img0001.jpg "Western Blot: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi)](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80846-img0003.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80846-img0004.jpg)
![Immunohistochemistry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi)](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80846-img0002.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80846-img0005.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0005.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Paraffin-embedded human melanoma. pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0004.jpg)
![Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Low-Endotoxin-Immunohistochemistry-Paraffin-NB100-65956-img0013.jpg "Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.")
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0008.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0009.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0010.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0011.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0012.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0004.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0005.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0006.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0006.jpg "Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0002.jpg)
![Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). conjugated with APC (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Flow-Cytometry-NBP2-45316-img0003.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0001.jpg)
![Immunofluorescence: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with CF594 (Cyan).The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunofluorescence-NBP2-45316-img0004.jpg)
![Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-western-blot-362024112212..jpg "Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112318..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112412..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112523..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112620..jpg)
![Flow Cytometry: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of HL-60 cells labeling MHC class I.Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-flow-cytometry-362024112742..jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue labeling MHC class I with Rabbit anti-MHC class I antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 c, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunocytochemistry-immunofluorescence-362024112932..jpg)