| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33084 | |||
| IHC-Prediluted | NBP2-44524 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47339SS | |||
| Azide and BSA Free | NBP2-80748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6106SS | |||
| Azide and BSA Free | NBP2-80778 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1397SS | |||
| Azide and BSA Free | NBP2-80991 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6125SS | |||
| Azide and BSA Free | NBP2-80901 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1762SS | |||
![Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0018.jpg "Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% (indicated by white arrowheads) and/or 30% (indicated by yellow arrowheads) at 30 min p.i. N = 10 with consistent results. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv976), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-395-img0020.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0016.jpg)
![Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by two other rabbit anti-MDC1 antibodies. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunoprecipitation-NB100-395-img0019.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After irradiation, cells were fixed & immunofluorescence stained for NBS1 (A) & quantified for cells with more than five nuclear foci (B). Bars, 10 μm. Error bars represent standard deviation from a triplicate of a representative experiment. DOX treated shBRUCE-U2OS cells were irradiated & the cell lysates subject to immunoblotting with the indicated antibodies showing the protein levels of ATM, MDC1 & NBS1 are not reduced post BRUCE knockdown (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26683461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-395_rabbit-polyclonal-mdc1-antibody-31020241534531.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-WA-1-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).")
![Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Western-Blot-NBP1-47339-img0006.jpg "Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0004.jpg)
![Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Flow-Cytometry-NBP1-47339-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunohistochemistry-Paraffin-NBP1-47339-img0005.jpg)
![Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Simple-Western-NBP1-47339-img0007.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0005.jpg "Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: hnRNP L Antibody (4D11) [NB120-6106] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NB120-6106-img0004.jpg)
![Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106]](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Immunohistochemistry-Frozen-NB120-6106-img0002.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Western blot for anti-hnRNP-L on HeLa cell extracts](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0001.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP L at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0003.jpg)
![Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-D45-Western-Blot-NB600-1397-img0005.jpg "Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.")
![Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-D45-Immunohistochemistry-Paraffin-NB600-1397-img0002.jpg)
![Flow (Intracellular): Transportin 1 Antibody (D45) [NB600-1397] - HeLa cells were stained with Transportin 1 NB600-1397 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-D45-Flow-Intracellular-NB600-1397-img0003.jpg)
![Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Transportin 1 (D45) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-D45-Western-Blot-NB600-1397-img0004.jpg)
![Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-D45-Immunohistochemistry-Paraffin-NB600-1397-img0001.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Western-Blot-NBP2-16521-img0021.jpg "Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.")
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse duodenum. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Chromatin Immunoprecipitation (ChIP): FOXO3 Antibody [NBP2-16521] - (D) ChIP assay for LMOD1 and EGFR (as a positive control region) in HCASMC chromatin lysates immunoprecipitated with antibodies to FOXO3 or a negative control rabbit IgG. n = 3. (E) Allele specific ChIP (haploChIP) for FOXO3 protein in DNA derived from cultured HCASMC ChIP experiments. DNA from cell line homozygous for the ancestral allele was used as a positive control and arbitrarily set to 1. Values represent mean +/- standard deviation of triplicates. Similar results were observed from n = 4 independent lines for each genotype. (F) Quantitative RT-PCR analysis of LMOD1 in HCASMCs following knock down of endogenous FOXO3 (F) or overexpression of FOXO3 (G). The results were reproduced in n = 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007755) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16521-img0030.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - 50 ug of mouse brain whole cell lysate). Antibody at 1:500.](http://images.novusbio.com/fullsize/FOXO3A-Antibody-Western-Blot-NBP2-16521-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/FOXO3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat colon. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0024.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0026.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-16521-img0027.jpg)
![Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - Jurkat whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ug of FOXO3A antibody 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunoprecipitation-NBP2-16521-img0029.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - LMOD1 expression is mediated by PDGF-BB-FOXO3 signaling cascade.(A) UCSC Browser screenshot of the LMOD1 CAD locus at chromosome 1q32.1 highlighting the candidate causal variant, rs34091558, overlapping ATAC-seq open chromatin & RNA-seq tracks in HCASMCs treated with PDGF-BB (n = 2 biological replicates). Genomic coordinates refer to hg19 assembly. Quantitative RT-PCR (B) & Western blotting (C) data revealing PDGF-BB & phosphorylated-FOXO3 (P-FOXO3) mediated LMOD1 expression. (D) Co-expression microarray analysis performed in a cohort of carotid atherosclerotic plaques (n = 127) indicating that LMOD1 & FOXO3 are positively correlated in arterial tissues. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30444878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-310202415345315.jpg)
![Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - Chemotherapy-induced A2BR expression promotes FOXO3 binding on pluripotency factor genes through decreased H3K27me3 & increased H3K27ac chromatin marks. A & B, MDA-MB-231 NTC or A2BR knockdown subclones were transfected with pLX304 (empty vector, EV) or pLX304 encoding A2BR. Cells were treated with vehicle (V) or 10 nM paclitaxel (P) for 72 h & chromatin immunoprecipitation (ChIP) was performed with antibody against FOXO3. Primers flanking the FOXO3 binding site in the NANOG, SOX2 & KLF4 gene (A) were used for qPCR (B; mean ± SEM; n = 3); **p Sample: Paraffin-embedded human glioblastoma.FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunohistochemistry-paraffin-2611202518191319.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-western-blot-2611202518303821.jpg)
![FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunocytochemistry-immunofluorescence-261120251845408.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-western-blot-261120251824390.jpg)
![FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunohistochemistry-paraffin-2611202518441020.jpg)
![Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Western-Blot-NB120-6125-img0003.jpg "Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Immunocytochemistry-Immunofluorescence-NB120-6125-img0005.jpg)
![Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Flow-Cytometry-NB120-6125-img0006.jpg)
![Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Western-Blot-NB120-6125-img0002.jpg)
![Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Western-Blot-NB100-292-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunohistochemistry-Paraffin-NB100-292-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunoprecipitation-NB100-292-img0018.jpg)
![Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Western-Blot-NB100-268-img0001.jpg "Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunoprecipitation-NB100-268-img0003.jpg)
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0007.jpg "Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0006.jpg)
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0001.jpg)
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0004.jpg)
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0002.jpg)
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0003.jpg)
![Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Simple-Western-NB100-1762-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Knockdown of KDM1A protein was confirmed by western blotting of whole-cell lysates from DAOY and ONS-76 cells. beta-actin served as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252778/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Knockdown-Validated-NB100-1762-img0008.jpg)
![Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0009.jpg "Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-471-img0004.jpg)
![Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0005.jpg)
![Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/WRN-Antibody-Immunoprecipitation-NB100-471-img0008.jpg)
![Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg)
![Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/p53-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB200-171-img0073.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0003.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg)