| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11038 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13600SS | |||
| Azide and BSA Free | NBP2-80924 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80589 | |||
| BSA Free | NB110-60521SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04965-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58116-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59677SS | |||
| Azide and BSA Free | NBP2-80856 | |||
![Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Western-Blot-H00009700-M01-img0005.jpg "Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Immunohistochemistry-Paraffin-H00009700-M01-img0004.jpg)
![Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Western-Blot-NBP3-11037-img0003.jpg "Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0010.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Human tonsil stained with Nucleolin Antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Analysis of human cells administered in pig brain using Nucleolin antibody. Antibody did NOT stain porcine tissue allowing the presence of human cells to be determined. Primary antibody dilution 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0009.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western blot analysis on different lysates with NBP2-67381 at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate (negative) Lane 3: A549 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67381 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67381_-western-blot-639204597490072015.jpg)
![Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunohistochemistry-Paraffin-NBP1-33506-img0003.jpg "Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-NBP1-33506-img0007.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-2611202518191318.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251830124.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251829022.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-26112025196217.jpg)
![Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPG-Antibody-Western-Blot-NB100-74611-img0010.jpg "Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XPG-Antibody-Immunohistochemistry-Paraffin-NB100-74611-img0011.jpg)
![Immunoprecipitation: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for IP at 6 ug per reaction. For blotting immunoprecipitated ERCC5/XPG, NB100-74611 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPG-Antibody-Immunoprecipitation-NB100-74611-img0009.jpg)
![Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Western-Blot-NB120-13600-img0005.jpg "Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.")
![Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Immunohistochemistry-Paraffin-NB120-13600-img0006.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Western Blot: BAP1 Antibody (1G8) [NB110-60521] - Detection of BAP1 in HeLa WCE.](http://images.novusbio.com/fullsize/BAP1-Antibody-1G8-Western-Blot-NB110-60521-img0005.jpg "Western Blot: BAP1 Antibody (1G8) [NB110-60521] - Detection of BAP1 in HeLa WCE.")
![Flow Cytometry: BAP1 Antibody (1G8) [NB110-60521] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using BAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BAP1-Antibody-1G8-Flow-Cytometry-NB110-60521-img0009.jpg)
![Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis in HeLa nuclear Extracts.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP2-24531-img0007.jpg "Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis in HeLa nuclear Extracts.")
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24531-img0005.jpg)
![Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis of hnRNPK in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this experiment.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP2-24531-img0002.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24531-img0004.jpg)
![Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0002.jpg "Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.")
![<b>Biological Strategies Validation. </b>Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 incubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nbp1-04965_rabbit-polyclonal-eif4g1-p-ser1232-antibody-103202523464542.jpg)
![Western Blot: RAD52 Antibody [NBP2-58116] - HCC1937 human breast cancer cells untreated (1) and treated with a PARP inhibitor for 96 h (2). Two RAD52 bands can be appreciated at around 55 kDa. Ponceau red staining is used as total protein loading control (below). Primary antibody dilution 1:500 (overnight, 4C, rotation). Image from verified customer review.](http://images.novusbio.com/fullsize/RAD52-Antibody-Western-Blot-NBP2-58116-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RAD52 Antibody [NBP2-58116] - HCC1937 human breast cancer cells untreated (1) and treated with a PARP inhibitor for 96 h (2). Two RAD52 bands can be appreciated at around 55 kDa. Ponceau red staining is used as total protein loading control (below). Primary antibody dilution 1:500 (overnight, 4C, rotation). Image from verified customer review.")
![Western Blot: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - Total protein from human HeLa, Jurkat, and mouse MEF and Neuro2A cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Mre11 in block buffer and detected with an anti-Armenian Hamster HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Western-Blot-NBP2-59677-img0001.jpg "Western Blot: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - Total protein from human HeLa, Jurkat, and mouse MEF and Neuro2A cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Mre11 in block buffer and detected with an anti-Armenian Hamster HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Mre11 (15B8.1E7.6) at 2 ug/ml overnight at 4C and detected with an anti-Armenian hamster IgG Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunocytochemistry-Immunofluorescence-NBP2-59677-img0003.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse prostate tissue section using 1:500 dilution of Mre11 antibody (clone 15B8.1E7.6). The signal was developed using HRP-DAB indirect detection method and the sections were counterstained using hematoxylin staining. This Mre11 antibody generated a strong nuclear with mild to moderate cytoplasmic staining in the glandular epithelial cells.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunohistochemistry-Paraffin-NBP2-59677-img0005.jpg)
![Flow Cytometry: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - An intracellular stain was performed on A431 cells with Mre11 [15B8.1E7.6] Antibody NBP2-59677B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Flow-Cytometry-NBP2-59677-img0006.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse prostate using 1:500 dilution of Mre11 antibody (clone 15B8.1E7.6). The signal was developed using HRP-DAB indirect detection method and the sections were counterstained using hematoxylin staining. This Mre11 antibody generated a strong nuclear positivity with considerable mild to moderate cytoplasmic staining in the glandular epithelial cells. Select cells depicted MRE11 foci which reflects towards the presence of DNA damage in those cells.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunohistochemistry-Paraffin-NBP2-59677-img0004.jpg)
![Flow (Intracellular): Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - An intracellular stain was performed on HeLa Cells with Mre11 (15B8.1E7.6) antibody NBP2-59677 (blue). Unstained cells are shown in orange. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by armenian hamster IgG Alexa Flour 488-conjugated secondary.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Flow-Intracellular-NBP2-59677-img0002.jpg)
