| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06609SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34663-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29617SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22128SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-617SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86635-25ul | |||
![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0024.jpg "Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibodyused for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30475] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP2-76410 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30475-img0022.jpg)
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0016.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NB100-87026-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0013.jpg)
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NB100-87026-img0015.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
![Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NBP1-06609-img0005.jpg)
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0009.jpg "Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0007.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.Positive control:Lane 1: HepG2 Lane 2: NIH-3T3Lane 3: MCF-7 Lane 4: Rat kidney tissue](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0002.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0004.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0006.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent analysis of HeLa cells using Ku70/XRCC6 antibody (KU729). Image from verified customer review.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Immunocytochemistry-Immunofluorescence-NBP2-34247-img0008.jpg "Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent analysis of HeLa cells using Ku70/XRCC6 antibody (KU729). Image from verified customer review.")
![Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Flow-Cytometry-NBP2-34247-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Immunocytochemistry-Immunofluorescence-NBP2-34247-img0004.jpg)
![Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Flow-Cytometry-NBP2-34247-img0002.jpg)
![Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/nbp2-34247_mouse-monoclonal-ku70-xrcc6-antibody-ku729-1772024936268.jpg)
![Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/nbp2-34247_mouse-monoclonal-ku70-xrcc6-antibody-ku729-17720241003213.jpg)
![Western Blot: TREX1 Antibody [NBP2-29617] - Analysis of TREX1 partial recombinant protein by using TREX1 antibody at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/TREX1-Antibody-Western-Blot-NBP2-29617-img0001.jpg "Western Blot: TREX1 Antibody [NBP2-29617] - Analysis of TREX1 partial recombinant protein by using TREX1 antibody at a concentration of 1ug/ml.")
![Immunohistochemistry-Frozen: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human normal stomach using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration. Specific cytoplasmic and nuclear staining was observed in the glandular cells/mucosal epithelium of stomach [Magnification 10X].](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Frozen-NBP2-29617-img0014.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of TREX1 protein in a transverse section of normal skeletal muscle from human using 7.5 ug/ml concentration of TREX1 antibody. Specific staining was observed in the cytoplasm and the nuclei of the myocytes.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0003.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human normal prostate using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration. Cytoplasmic and nuclear staining was observed in the stromal as well as glandular cells/epithelial cell of prostate. [Magnification 40X].](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0006.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human normal skin using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration. Specific cytoplasmic and nuclear staining was observed in all the epidermal cells and some cells of the dermal layer [Magnification 40X].](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0007.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human bladder cancer using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0008.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0009.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Aanalysis of FFPE tissue section of human hepatic/liver carcinoma using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0010.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human uterine cancer using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0011.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0012.jpg)
![Immunohistochemistry-Paraffin: TREX1 Antibody [NBP2-29617] - Analysis of FFPE tissue section of human rectal adenocarcinoma using rabbit polyclonal TREX1 antibody at 7.5 ug/ml concentration.](http://images.novusbio.com/fullsize/TREX1-Antibody-Immunohistochemistry-Paraffin-NBP2-29617-img0013.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0006.jpg "Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.")
![Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-22128-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0005.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0002.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0004.jpg)
![ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-ELISA-NBP2-22128-img0001.jpg)
![Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW109-Western-Blot-NB100-617-img0003.jpg "Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.")
![Western Blot: DNA-PKcs Antibody [NB100-658] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954 whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-658) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0025.jpg "Western Blot: DNA-PKcs Antibody [NB100-658] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954 whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-658) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-658] - Detection of Human DNA-PKcs in HeLa whole cell lystate using NB100-658. DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-659 and NB100-660.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0023.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-658] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. DNA-PKcs antibody at 1:1000 dilution O/N. Secondary antibody: HRP-conjugated Donkey anti-rabbit IgG polyclonal antibody at 1:2000 dilution. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0024.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-658] - Molecular Characterization of G1 Resection. End joining events in GC92 cells treated with siKu70/80, siLig4, siLig1/3, or DNA-PKcs antibody. Data are mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5316416/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Knockdown-Validated-NB100-658-img0026.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-658] - Molecular Characterization of G1 Resection(A) Schematic of the NHEJ reporter assay. The repair of two I-SceI-induced DSBs can result in loss of the intervening fragment, which is detected by a CD4+ signal (Rass et al., 2009). CD4+ clones were amplified by PCR (green arrows) across the repair site & sequenced. Repair of the two DSBs can also occur without loss of the intervening fragment, which escapes detection.(B) gamma H2AX foci in GC92 WT & Artemis KO cells treated with siDNA-PKcs or siCtIP. Cells were transfected with I-SceI, & foci were scored in I-SceI+ & I-SceI− cells (identified by immunofluorescence [IF] against I-SceI). Data are mean ± SEM.(C) End joining events in GC92 WT & Artemis KO cells containing the NHEJ reporter substrate. Cells were transfected with RFP or cMyc-Artemis constructs. Events were quantified by the fraction of CD4+ & RFP/cMyc+cells relative to all RFP/cMyc+cells, & results were normalized to WT cells. Data are mean ± SEM.(D) End joining events in GC92 WT & Artemis KO cells treated with siCtIP. Data are mean ± SEM.(E) Distribution of deletion sizes obtained from the sequence analysis of GC92 WT & siLig1/3-treated cells. nt, nucleotide.(F) End joining events in GC92 cells treated with siKu70/80, siLig4, siLig1/3, or siDNA-PKcs. Data are mean ± SEM.See also Table S1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28132842), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-658_rabbit-polyclonal-dna-pkcs-antibody-31020241682113.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31294 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. HDAC1 antibody (NBP1-31294) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg "Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86635_rabbit-polyclonal-smc4-antibody-30420251371429.jpg)
