| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81927-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87822-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90012-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97475-0.2mg | |||
| BSA Free | NBP1-97475-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1920SS | |||
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31294 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. HDAC1 antibody (NBP1-31294) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-2342024155517.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.")
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-234202415232510.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0014.jpg "Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunocytochemistry-Immunofluorescence-H00011004-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunohistochemistry-Paraffin-H00011004-M01-img0010.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi ( Cat # H00011004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01), clone 1G2 (Cat # H00011004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0012.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody (M01), clone 1G2.Lane 1: KIF2C transfected lysate(81.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0015.jpg)
![Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C transfected lysate using anti-KIF2C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KIF2C MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunoprecipitation-H00011004-M01-img0011.jpg)
![Sandwich ELISA: KIF2C Antibody (1G2) [H00011004-M01] - Detection limit for recombinant GST tagged KIF2C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Sandwich-ELISA-H00011004-M01-img0013.jpg)
![Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Western-Blot-H00004176-M01-img0012.jpg "Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunocytochemistry-Immunofluorescence-H00004176-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunohistochemistry-Paraffin-H00004176-M01-img0010.jpg)
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0005.jpg)
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0006.jpg)
![ELISA: MCM7 Antibody (6C2) [H00004176-M01] - Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-ELISA-H00004176-M01-img0011.jpg)
![Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Western-Blot-NBP1-87822-img0012.jpg "Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87822-img0007.jpg)
![Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human skeletal muscle shows moderate positivity in nuclear membrane in a small subset of myocytes.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunohistochemistry-Paraffin-NBP1-87822-img0018.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-TMPO antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Western-Blot-NBP1-87822-img0011.jpg)
![Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human lymph node shows strong positivity in nuclear membrane in germinal center cells.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunohistochemistry-Paraffin-NBP1-87822-img0013.jpg)
![Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunohistochemistry-Paraffin-NBP1-87822-img0014.jpg)
![Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human small intestine shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunohistochemistry-Paraffin-NBP1-87822-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining in human lymph node and skeletal muscle tissues using NBP1-87822 antibody. Corresponding TMPO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thymopoietin-LAP2-Antibody-Immunohistochemistry-Paraffin-NBP1-87822-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human placenta shows very strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0018.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0019.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0016.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-90012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BAF57-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90012-img0009.jpg "Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-90012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human endometrium shows high expression.](http://images.novusbio.com/fullsize/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0010.jpg)
![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0011.jpg)
![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0001.jpg)
![Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/BAF57-Antibody-Western-Blot-NBP1-90012-img0008.jpg)
![Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-97475-img0002.jpg "Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.")
![Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0010.jpg "Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1920-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:750 conc. of Nucleolin antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed in normal cells and nuclear staining was observed in cancer cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-Paraffin-NB100-1920-img0009.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg)
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg)
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RPA2-Antibody-Western-Blot-NB100-332-img0041.jpg "Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NB100-332-img0040.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 Antibody [NB100-332] - Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for IP at 3 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (NB100-23017). For blotting immunoprecipitated Phospho RPA32 (S4/S8), NB100-23017 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunoprecipitation-NB100-332-img0042.jpg)