| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38600-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37828-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-508SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47861-0.1mg | |||
| IHC-Prediluted | NBP2-48319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1031SS | |||
![Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SFPQ-Antibody-Immunohistochemistry-Paraffin-NBP2-20329-img0003.jpg "Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded mouse uterus, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SFPQ-Antibody-Immunohistochemistry-Paraffin-NBP2-20329-img0004.jpg)
![Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-910202420115084.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunohistochemistry-paraffin-261120251965224.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunocytochemistry-immunofluorescence-261120251847423.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunohistochemistry-paraffin-261120251824169.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80867-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80867-img0003.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80867-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80867-img0002.jpg)
![ELISA: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-ELISA-NBP2-80867-img0005.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Simple-Western-NBP2-80867-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Knockout-Validated-NBP2-80867-img0006.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0016.jpg "Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0013.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining in human testis and liver tissues using anti-POLB antibody. Corresponding POLB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38600-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining in human testis and liver tissues using anti-POLB antibody. Corresponding POLB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38600-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38600-img0005.jpg)
![Immunohistochemistry: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-NBP2-38600-img0002.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0019.jpg "Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0012.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0015.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0008.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0018.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0013.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0014.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0006.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0007.jpg)
![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0005.jpg "Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.")
![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0004.jpg)
![Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunoprecipitation-NB100-508-img0003.jpg)
![Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA70-Antibody-Western-Blot-NB100-2204-img0015.jpg "Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No.used at a dilution of 1:1,000 (1 ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml (right). Detection: DAB](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunohistochemistry-Paraffin-NB100-2204-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody NB100-2204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA70-Antibody-Western-Blot-NB100-2204-img0014.jpg)
![Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- RPA70 Lot2 used at a dilution of 1:1,000 (1 ug/ml) (left) and Lot3 used at a dilution of1:5,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunohistochemistry-NB100-2204-img0012.jpg)
![Immunoprecipitation: RPA70 Antibody [NB100-2204] - Detection of human RPA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPA70 antibody NB100-2204 (lot NB100-2204-3) used for IP at 6 ug per reaction. RPA70 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2204-2). For blotting immunoprecipitated RPA70, NB100-2204 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunoprecipitation-NB100-2204-img0017.jpg)
![Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NB100-2312-img0016.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody NB100-2312 (lot NB100-2312-3) used for IP at 6 ug per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot NB100-2312-2). For blotting immunoprecipitated FOXO1a, NB100-2312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunoprecipitation-NB100-2312-img0015.jpg)
![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0003.jpg "Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. DNA ligase III dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0003.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0004.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0001.jpg)
![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg "Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)