| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-108SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-481SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NLS1531AF405 | |||
| Alexa Fluor 488 | NLS1531AF488 | |||
| Alexa Fluor 647 | NLS1531AF647 | |||
| Alexa Fluor 700 | NLS1531AF700 | |||
| Biotin | NLS1531B | |||
| DyLight 405 | NLS1531V | |||
| DyLight 488 | NLS1531G | |||
| DyLight 550 | NLS1531R | |||
| DyLight 650 | NLS1531C | |||
| FITC | NLS1531F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36429-0.025mg | |||
| Azide and BSA Free | NBP2-81009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61640-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43262-0.5mg | |||
| BSA Free | NBP1-43262-25ug | |||
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, and caspases 3, 8, and 9) and inflammation (TNF-a) in the heart of hypertensive rats (A) SHR and (B) DOCA-salt, and rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected and cropped by Citeab from the following publication (The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension. Molecules (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Western-Blot-NBP1-05123-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, and caspases 3, 8, and 9) and inflammation (TNF-a) in the heart of hypertensive rats (A) SHR and (B) DOCA-salt, and rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected and cropped by Citeab from the following publication (The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension. Molecules (2018) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Immunohistochemistry-Paraffin-NBP1-05123-img0002.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - AML12 whole cell lysate. Good specific band is observed. Primary antibody at 1: 2000, secondary antibody at 1: 20000. Skimmed milk as blocking buffer. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Western-Blot-NBP1-05123-img0003.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-31020241534352.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-310202415394532.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-31020241535665.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0005.jpg "Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-108] - NTH1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-108-img0006.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma using NTH1 antibody at 5ug/ml concentration (1:200 dilution). The primary antibody binding to NTH1/ NTHL1 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0014.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0002.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of NTH1 antibody. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0013.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney cancer using 5 ug/ml concentration of NTH1 antibody. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0012.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0011.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0010.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0004.jpg "Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.")
![Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunohistochemistry-Frozen-NB100-481-img0005.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0007.jpg)
![Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunoprecipitation-NB100-481-img0006.jpg)
![Immunohistochemistry: Mas Antibody [NLS1531] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-NLS1531-img0001.jpg "Immunohistochemistry: Mas Antibody [NLS1531] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.")
![Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Representative immunolocalisation images of AT1R, AT2R, MasR, and ACE2 in adult WKY rats and aged WKY rats. Mean data for aortic expression of the AT1R expressed as relative fluorescent units in adult (n = 5) and aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijht/2012/192567/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-Paraffin-NLS1531-img0003.jpg)
![Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Analaysis of Brain, Neurons and Glia using NLS1531 at 15 ug/ ml.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-Paraffin-NLS1531-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - (a) Representative immunolocalisation images of AT1R, AT2R, MasR, & ACE2 in adult WKY rats & aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, & (e) ACE2 expressed as relative fluorescent units in adult (n = 5) & aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22187625), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1531_rabbit-polyclonal-mas-antibody-imgenex-img-71590-31020241517130.jpg)
![Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - Immunofluorescence studies in HEK293T cells overexpressing c-myc tagged MasR using MasR antibodies.Images of fluorescence signal corresponding to secondary cyanine (Cy3)-conjugated antibody (A), nuclear Hoechst 33258 staining (B), merged images (C) & bright field (D) are shown. In cells transfected with the pcDNA 3.1/c-myc-MasR construct, antibodies NLS-1531 & sc-54682 generated a similar staining pattern to that generated with the anti c-myc antibody. No staining was observed with any of these antibodies in cells transfected with the empty vector pcDNA 3.1. Antibody sc-135063 was capable of staining the plasma membrane of cells overexpressing the MasR but also generated widespread signals in cells transfected with the empty vector. For antibody AAR-013 there was no staining associated with the cell membrane. Intense immunocytochemical staining of identical distribution & intensity was revealed in cells transfected with the empty vector & those transfected with the c-myc-MasR construct. Images are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1531_rabbit-polyclonal-mas-antibody-imgenex-img-71590-310202416121931.jpg)
![Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] - Immunohistochemistry in heart & kidney from wild-type (WT) & MasR-KO mice.Negative controls performed by omitting the primary antibody demonstrated minimal background immunostaining in heart (A) & kidney (B) sections. (A) In heart sections, NLS-1531 antibody stained predominantly the cytoplasm of cardiomyocytes with similar intensity in WT & MasR-KO hearts. The AAR-013 antibody stained the cardiomyocytes nucleus & weaker staining was observed in their cytoplasm. The same pattern was found in heart sections of MasR-KO mice. (B) In kidney sections, for NLS-1531 antibody, staining was mostly restricted to cytoplasm of numerous tubules cells with similar intensity in WT & MasR-KO kidneys. Antibody AAR-013 stained most intensely tubules cells nucleus & weaker staining was observed in their cytoplasm. Glomeruli were also stained positively. The same pattern was found in kidney sections of MasR-KO. Preincubation of the AAR-013 antibody with the blocking peptide provided by the vendors eliminated the immunohistochemical nuclear staining both in WT & MasR-KO mice kidney sections. Images are representative of n = 3. Bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1531_rabbit-polyclonal-mas-antibody-imgenex-img-71590-31020241612234.jpg)
![Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Shugoshin-Antibody-3C11-Western-Blot-H00151648-M01-img0002.jpg "Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 transfected lysate using anti-SGOL1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGOL1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Shugoshin-Antibody-3C11-Immunoprecipitation-H00151648-M01-img0001.jpg)
![Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Western-Blot-NBP2-67416-img0012.jpg "Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunocytochemistry-Immunofluorescence-NBP2-67416-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse lung tissue. IF-stained paraffin section. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0010.jpg)
![Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image submitted by a verfied customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Western-Blot-NBP2-67416-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunocytochemistry-Immunofluorescence-NBP2-67416-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0007.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Western-Blot-NBP2-36429-img0018.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-NBP2-36429-img0012.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0017.jpg)
![Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Flow-Cell-Surface-NBP2-36429-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0013.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0014.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0015.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0016.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0040.jpg "Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunocytochemistry-Immunofluorescence-NB110-61640-img0034.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0044.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0031.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0039.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0041.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0042.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0043.jpg)
![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-NB100-563-img0006.jpg "Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0007.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0020.jpg "Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Mouse (B7-H1) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0019.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0021.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0011.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0010.jpg)
![Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Western-Blot-NB120-3577-img0005.jpg "Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.")
![Immunocytochemistry/Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577] - Primate amygdala.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3577-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577] - Figure 1 illustrates immunolocalization of ER beta in primate amygdala using NB120-3577.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Immunohistochemistry-Paraffin-NB120-3577-img0004.jpg)