| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-78644SS | |||
| Azide and BSA Free | NBP2-80781 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77477SS | |||
| Azide and BSA Free | NBP2-80624 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-113SS | |||
| Azide and BSA Free | NBP2-80853 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77456SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78978SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05423-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg "Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20535_rabbit-polyclonal-sod2-mn-sod-antibody-1610202419405789.jpg)
![Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Somatostatin-R2-SSTR2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-157-img0005.jpg "Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0004.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0003.jpg)
![Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-FDO66Q-Immunohistochemistry-NB110-78644-img0002.jpg "Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg)
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0007.jpg "Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0009.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0006.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue)Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0011.jpg)
![Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Flow-Cytometry-NB200-113-img0012.jpg)
![Western Blot: SERCA2 ATPase Antibody [NB100-237] - Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 and 200 ug/mL) (A) or to similar dose (500 ug/ml) of LDL or HDL (B) for 24 hours. HL-1 cells incubated in absence of LDL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23516438) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NB100-237-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody [NB100-237] - Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 and 200 ug/mL) (A) or to similar dose (500 ug/ml) of LDL or HDL (B) for 24 hours. HL-1 cells incubated in absence of LDL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23516438) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of human and mouse SERCA2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody NB100-237 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NB100-237-img0001.jpg)
![Western Blot: SERCA2 ATPase Antibody [NB100-237] - LDL reduces SERCA expression & SR calcium loading.Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 & 200 µg/mL) (A) or to similar dose (500 µg/ml) of LDL or HDL (B) for 24 hours. The bar graph below shows SERCA2 band quantification. Unchanged levels of beta -tubulin are shown as loading control. Results are mean±SEM of three independent experiments performed in duplicate. *P<0.05 versus. HL-1 cells incubated in absence of LDL. C Calcium images acquired at rest before (CON) & during a rapid caffeine application. D Representative calcium transients recorded in cultures incubated without lipoproteins (CON), with 500 µg/ml LDL or with 500 µg/ml HDL. The lower panel shows the corresponding time integrals of the calcium transients. E summary of the effect of LDL & HDL on the time integral of the caffeine induced calcium transient. *P<0.05 vs. cells incubated with LDL Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-237_rabbit-polyclonal-serca2-atpase-antibody-31020241683920.jpg)
![Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Western-Blot-NBP1-77456-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.")
![Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-NBP1-77456-img0003.jpg)
![Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Simple-Western-NBP1-77456-img0005.jpg)
![Total IRS-1, p-IRS-1 and downstream substrates level in blood neuron-derived extracellular vesicles. (A) The representative protein blot images of IRS-1 and different phosphorylated forms of IRS-1, including Y612, S616, S636/639, and S312. HSP70 and CD63 were exosomal proteins and markers. GAPDH was the protein loading control (1 and 2 indicate different samples). Comparison of the levels of total IRS-1, p-IRS-1Y612, p-IRS-1S616, p-IRS-1S636/639, p-IRS-1S312(B), and downstream IRS-1 substrates (C–E) between healthy controls [with or without diabetes mellitus (DM)] and Parkinson’s disease (PD) patients (with or without DM). The phosphorylation status of IRS-1, P70S6K, Akt, and Erk was normalized to total form IRS-1, P70S6K, Akt, and Erk. (F) The EV markers–CD63 and HSP70 was not different between 4 groups. Data was presented as mean +/- SEM. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33344443), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77456_rabbit-polyclonal-hsp70-hspa1a-antibody-western-blot-13220261535269.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-78978-img0003.jpg "Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78978] - Tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78978-img0002.jpg)
![Western Blot: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis using IGF1R-Beta mouse mAb against truncated IGF1R recombinant protein.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-3G5C1-Western-Blot-NB110-87052-img0021.jpg "Western Blot: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis using IGF1R-Beta mouse mAb against truncated IGF1R recombinant protein.")
![Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) [NB110-87052] - Expression of growth factor receptors in male breast cancer. Expression of HER2, FGFR2, EGFR, IGF-I R/IGF1R, and MET in representative cases of male breast cancer. Size bars equal 20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0053353) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-3G5C1-Immunohistochemistry-NB110-87052-img0022.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of ovarian cancer tissues using IGF1R-Beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-3G5C1-Immunohistochemistry-Paraffin-NB110-87052-img0019.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of rectum cancer tissues using IGF1R-Beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-3G5C1-Immunohistochemistry-Paraffin-NB110-87052-img0020.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052] - Representative IHC staining in primary breast tumors (left; no membrane staining for Claudin-4, CAIX & GLUT-1 (significant negative to positive conversion), positive membrane staining for EGFR & IGF1R (negative to positive conversion n.s.) & metastases (right; positive membrane staining). Magnification: 20× (inlet 40×). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25417118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-87052_mouse-monoclonal-igf-i-r-igf1r-antibody-3g5c1-310202415382448.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg "Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg)
![Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg)
![Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.](http://images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg)
![Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg)
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 and -58E7 upregulated CIP2A mRNA and protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 and -58E7 upregulated CIP2A mRNA and protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-68264] - Human colon carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-Paraffin-NB100-68264-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CIP2A Antibody [NB100-68264] - Detection of Human CIP2A on HeLa whole cell lysate using NB100-68264. CIP2A was also immunoprecipitated by rabbit anti-CIP2A antibody NB100-68263.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation and DNA synthesis of HPV-16E7-expressing cells with CIP2A siRNA for 48 hr. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Knockdown-Validated-NB100-68264-img0004.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 & -58E7 upregulated CIP2A mRNA & protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7; & (B) Quantification. (C) qRT-PCR analysis of CIP2A mRNA level in PHKs expressing HPV-16E7, -58E7, -6E7. (D) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7 & 16E7 mutants L22A & C24G. Babe, vector control. *, P < 0.05; **, P < 0.01; & ***, P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415291953.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Silencing CIP2A caused decreased Cdk1 & Cdk2 proteins in 16E6‐expressing cells. A, Western blot analysis of CIP2A, Cdk4, Cdk6, cyclin D1, Cdk1, Cdk2, cyclin B1, cyclin A2 & cyclin E1 protein levels in cells expressing HPV‐16E6 transfected with CIP2A siRNA & then treated with PBS or 10 μg/mL bleomycin for 24 h. A representative of 3 independent experiments is shown. B, Quantification of all cell cycle‐related proteins. Data from 3 experiments are summarized. C, Relative mRNA levels of all cell cycle‐related genes determined by qRT‐PCR. Data from 3 experiments are summarized. *, P < .05; **, P < .01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415395935.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of Cdk1 & Cdk2 by CIP2A knockdown in cervical cancer SiHa cells caused G1 arrest. A, Western blot analysis of 16E6, p53 & CIP2A after HPV‐16E6 knockdown in cervical cancer SiHa cells. B, Protein expression of CIP2A, B‐Myb, Cdk1 & Cdk2 in SiHa cells after CIP2A knockdown. Data from a representative of 3 experiments are shown. C, Flow cytometric analysis of SiHa cells with CIP2A knockdown treated with PBS or bleomycin. A representative flow cytometry of 3 independent experiments is shown. D, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. *, P < .05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415334911.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation & DNA synthesis of HPV-16E7-expressing cells(A) Western blot analysis of protein level of 16E7 & CIP2A in RPE1-16E7 cells & (B) with CIP2A siRNA for 48 hr. (C) CCK8 assay of cell proliferation of RPE1-16E7 cells with CIP2A siRNA. (D) Flow cytometry of cells with CIP2A siRNA & labeled with BrdU for 2 hr, then stained with PI & BrdU; & (E), Quantification. Babe, vector control. **, P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241534369.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of Cdk1 & Cdk2 by CIP2A knockdown in cervical cancer SiHa cells caused G1 arrest. A, Western blot analysis of 16E6, p53 & CIP2A after HPV‐16E6 knockdown in cervical cancer SiHa cells. B, Protein expression of CIP2A, B‐Myb, Cdk1 & Cdk2 in SiHa cells after CIP2A knockdown. Data from a representative of 3 experiments are shown. C, Flow cytometric analysis of SiHa cells with CIP2A knockdown treated with PBS or bleomycin. A representative flow cytometry of 3 independent experiments is shown. D, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. *, P < .05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415395929.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of CIP2A by siRNA impeded cell viability & DNA synthesis in HPV‐16E6–expressing cells. A, Elevated expression of CIP2A protein in 16E6‐expressing RPE1 cells. B, Western blot analysis of CIP2A & p53 proteins after transfection with scrambled siRNA (siCon) or CIP2A siRNA (siCIP2A) for 48 h. A representative of 3 independent experiments is shown. C, Cell viability assay of RPE1‐16E6 cells with CIP2A knockdown. D, Representative flow cytometry of BrdU staining profiles is shown. E, The mean & SD of BrdU‐positive cells from 3 experiments are summarized. **, P < .01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415384150.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - CIP2A siRNA knockdown caused G1 arrest & decreased Cdk1 & Cdk2 protein levels in E7-expressing cells(A) Flow cytometry of cells expressing 16E7 transfected with CIP2A siRNA for 48 hr, treated with DMSO control or bleomycin (10 μg/mL) for 24 hr, then stained with PI. G1, S & G2 phases are indicated. (B) Western blot analysis of Cdk1, Cdk2, Cyclin A2, Cdk4, Cdk6, Cyclin D1 protein levels in cells expressing HPV-16E7 transfected with CIP2A siRNA. Babe, vector control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241534317.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation & DNA synthesis of HPV-16E7-expressing cells(A) Western blot analysis of protein level of 16E7 & CIP2A in RPE1-16E7 cells & (B) with CIP2A siRNA for 48 hr. (C) CCK8 assay of cell proliferation of RPE1-16E7 cells with CIP2A siRNA. (D) Flow cytometry of cells with CIP2A siRNA & labeled with BrdU for 2 hr, then stained with PI & BrdU; & (E), Quantification. Babe, vector control. **, P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-3102024153564.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of CIP2A by siRNA impeded cell viability & DNA synthesis in HPV‐16E6–expressing cells. A, Elevated expression of CIP2A protein in 16E6‐expressing RPE1 cells. B, Western blot analysis of CIP2A & p53 proteins after transfection with scrambled siRNA (siCon) or CIP2A siRNA (siCIP2A) for 48 h. A representative of 3 independent experiments is shown. C, Cell viability assay of RPE1‐16E6 cells with CIP2A knockdown. D, Representative flow cytometry of BrdU staining profiles is shown. E, The mean & SD of BrdU‐positive cells from 3 experiments are summarized. **, P < .01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415284523.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Regulation of Cdk1 & Cdk2 by CIP2A is dependent on B‐Myb rather than c‐Myc. A, Western blot analysis of CIP2A, c‐Myc, phospho‐S62‐Myc & B‐Myb protein levels in 16E6‐expressing cells after CIP2A knockdown. beta ‐Tubulin was used as a loading control. B, Protein levels of B‐Myb, c‐Myc & phospho‐S62‐Myc in 16E6‐expressing PHKs & (C) RPE1 cells. D, Protein levels of B‐Myb, Cdk1 & Cdk2, CIP2A & p53 in 16E6‐expressing cells after B‐Myb knockdown with siRNA. Data from a representative of 3 experiments are shown. E, Knockdown of B‐Myb down‐regulates Cdk1 & Cdk2 luciferase reporter activities. RPE1 cells were cotransfected with the Cdk1 or Cdk2 promoter‐luciferase constructs & renilla luciferase control plasmid together with B‐Myb siRNA plasmid. Cells were harvested after 48 h, & lysates were assayed for luciferase activity. F, Flow cytometric analysis of 16E6‐expressing cells transfected with B‐Myb siRNA treated with PBS or bleomycin. G1, S & G2 phases are indicated. A representative flow cytometry of 3 independent experiments is shown. G, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. H, Western blot analysis of B‐Myb, Cdk1 & Cdk2 in B‐Myb–overexpressing CIP2A knockdown cells. Data from a representative of 3 experiments are shown. *, P < .05; ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241612638.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Induction of CIP2A mRNA & protein expression by HPV‐16E6 in PHKs. A, mRNA expression of HPV‐16E6 in PHKs expressing 16E6 & F2V using beta ‐actin as a loading control. B, Protein levels of HPV‐16E6, p53 & p21 in PHKs expressing 16E6 & F2V. Expression of GAPDH was used as a loading control. A representative of 2 independent experiments is shown. C, HPV‐16E6 expression leads to increased protein expression of CIP2A in PHKs. Data from a representative of 3 experiments are shown. D, Data from 3 experiments are summarized. E, Relative CIP2A mRNA expression was determined by qRT‐PCR in the above cells. Data from 3 experiments are summarized. The mean & standard deviation (SD) of 3 independent experiments are shown. Babe, pBabe‐puromycin vector. *, P < .05; **, P < .01; & ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-3102024161263.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Regulation of Cdk1 & Cdk2 by CIP2A is dependent on B‐Myb rather than c‐Myc. A, Western blot analysis of CIP2A, c‐Myc, phospho‐S62‐Myc & B‐Myb protein levels in 16E6‐expressing cells after CIP2A knockdown. beta ‐Tubulin was used as a loading control. B, Protein levels of B‐Myb, c‐Myc & phospho‐S62‐Myc in 16E6‐expressing PHKs & (C) RPE1 cells. D, Protein levels of B‐Myb, Cdk1 & Cdk2, CIP2A & p53 in 16E6‐expressing cells after B‐Myb knockdown with siRNA. Data from a representative of 3 experiments are shown. E, Knockdown of B‐Myb down‐regulates Cdk1 & Cdk2 luciferase reporter activities. RPE1 cells were cotransfected with the Cdk1 or Cdk2 promoter‐luciferase constructs & renilla luciferase control plasmid together with B‐Myb siRNA plasmid. Cells were harvested after 48 h, & lysates were assayed for luciferase activity. F, Flow cytometric analysis of 16E6‐expressing cells transfected with B‐Myb siRNA treated with PBS or bleomycin. G1, S & G2 phases are indicated. A representative flow cytometry of 3 independent experiments is shown. G, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. H, Western blot analysis of B‐Myb, Cdk1 & Cdk2 in B‐Myb–overexpressing CIP2A knockdown cells. Data from a representative of 3 experiments are shown. *, P < .05; ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415535140.jpg)