| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65125SS | |||
| Azide and BSA Free | NBP2-80742 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-353SS | |||
| Azide and BSA Free | NBP2-80606 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80671 | |||
| BSA Free | NB100-136SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41291-0.025mg | |||
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A downregulation reduces proliferation and induces apoptosis in melanoma cells. Immunoblot analysis showing reduction in cancerous inhibitor of protein phosphatase 2A protein levels 48 h post siRNA transfection and effects on proliferation and apoptosis markers. The figure is representative of at last three independent biological experiments. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.425), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-74663-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-74663] - CIP2A downregulation reduces proliferation and induces apoptosis in melanoma cells. Immunoblot analysis showing reduction in cancerous inhibitor of protein phosphatase 2A protein levels 48 h post siRNA transfection and effects on proliferation and apoptosis markers. The figure is representative of at last three independent biological experiments. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.425), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: CIP2A Antibody [NB100-74663] - Characterization of CIP2A expression in a panel of normal human melanocytes (NHM) and melanoma cell lines. Cytoplasmic and nuclear expression of CIP2A in normal melanocytes, primary and metastatic melanoma cell lines assessed by immunohistochemical staining. CIP2A = cancerous inhibitor of protein phosphatase 2A. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.425), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-NB100-74663-img0012.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - Characterization of CIP2A expression in a panel of normal human melanocytes (NHM) and melanoma cell lines. Immunoblot analysis of protein expression levels of CIP2A. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.425), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-74663-img0009.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - Characterization of CIP2A expression in a panel of normal human melanocytes (NHM) and melanoma cell lines. Cytoplasmic and nuclear expression of CIP2A in normal melanocytes, primary and metastatic melanoma cell lines assessed by immunoblot analysis. CIP2A = cancerous inhibitor of protein phosphatase 2A. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.425), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-74663-img0010.jpg)
![Immunohistochemistry: CIP2A Antibody [NB100-74663] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-NB100-74663-img0008.jpg)
![Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-74663] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-Paraffin-NB100-74663-img0006.jpg)
![Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-74663] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-Paraffin-NB100-74663-img0007.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A is an inhibitor of PP2A-mediated DAPK dephosphorylationRSC96 cells were transfected with control, CIP2A siRNA (A), DKK-CIP2A (B), or DAPK siRNA (C) for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. PP2A activity & immunoblotting evaluations of DKK, CIP2A, pDAPK, & DAPK were performed. (D) RSC96 cells were incubated with GAS6 (100 ng/ml) for 30 min & co-immunoprecipitation between CIP2A, pDAPK & PP2A was evaluated. Immunoprecipitated PP2A activity was measured. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01, vs. control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415384132.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A is an inhibitor of PP2A-mediated DAPK dephosphorylationRSC96 cells were transfected with control, CIP2A siRNA (A), DKK-CIP2A (B), or DAPK siRNA (C) for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. PP2A activity & immunoblotting evaluations of DKK, CIP2A, pDAPK, & DAPK were performed. (D) RSC96 cells were incubated with GAS6 (100 ng/ml) for 30 min & co-immunoprecipitation between CIP2A, pDAPK & PP2A was evaluated. Immunoprecipitated PP2A activity was measured. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01, vs. control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415171316.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - GAS6 increases the amount of both CIP2A & p-DAPK in RSC96 cells(A) RSC96 cells were incubated with GAS6 (100 ng/ml), & immunoblotting evaluations of CIP2A & p-DAPK expression were performed. The lower panel shows the intensity during basal conditions from the scanned imaged (upper panel). (B) RSC96 cells incubated with & without GAS6 (100 ng/ml) for 30 min & analysed by immunofluorescence. (C) Immunohistochemistry images demonstrated that CIP2A expression levels were higher than sham levels at 14 days & 28 days in the cavernous nerve after BCNI. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415304268.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A is an inhibitor of PP2A-mediated DAPK dephosphorylationRSC96 cells were transfected with control, CIP2A siRNA (A), DKK-CIP2A (B), or DAPK siRNA (C) for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. PP2A activity & immunoblotting evaluations of DKK, CIP2A, pDAPK, & DAPK were performed. (D) RSC96 cells were incubated with GAS6 (100 ng/ml) for 30 min & co-immunoprecipitation between CIP2A, pDAPK & PP2A was evaluated. Immunoprecipitated PP2A activity was measured. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01, vs. control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415382441.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - GAS6 triggers p-Axl, p-DAPK & CIP2A to form a protein complex in RSC96 cells(A-B) Co-immunoprecipitation between p-Axl, p-DAPK & CIP2A with GAS6 stimulation for 30 min in RSC96 cells. Total lysate indicates 1/10 input in each experiment. The relative quantification of protein expression was normalized with respect to beta -actin expression. (C) Immunofluorescence image demonstrating co-localized CIP2A & p-Axl & co-localized p-Axl & p-DAPK (D) after GAS6 stimulation for 30 min in RSC96 cells. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-31020241539737.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A is an inhibitor of PP2A-mediated DAPK dephosphorylationRSC96 cells were transfected with control, CIP2A siRNA (A), DKK-CIP2A (B), or DAPK siRNA (C) for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. PP2A activity & immunoblotting evaluations of DKK, CIP2A, pDAPK, & DAPK were performed. (D) RSC96 cells were incubated with GAS6 (100 ng/ml) for 30 min & co-immunoprecipitation between CIP2A, pDAPK & PP2A was evaluated. Immunoprecipitated PP2A activity was measured. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01, vs. control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415175242.jpg)
![Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-74663] - GAS6 increases the amount of both CIP2A & p-DAPK in RSC96 cells(A) RSC96 cells were incubated with GAS6 (100 ng/ml), & immunoblotting evaluations of CIP2A & p-DAPK expression were performed. The lower panel shows the intensity during basal conditions from the scanned imaged (upper panel). (B) RSC96 cells incubated with & without GAS6 (100 ng/ml) for 30 min & analysed by immunofluorescence. (C) Immunohistochemistry images demonstrated that CIP2A expression levels were higher than sham levels at 14 days & 28 days in the cavernous nerve after BCNI. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415394520.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - CIP2A is an inhibitor of PP2A-mediated DAPK dephosphorylationRSC96 cells were transfected with control, CIP2A siRNA (A), DKK-CIP2A (B), or DAPK siRNA (C) for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. PP2A activity & immunoblotting evaluations of DKK, CIP2A, pDAPK, & DAPK were performed. (D) RSC96 cells were incubated with GAS6 (100 ng/ml) for 30 min & co-immunoprecipitation between CIP2A, pDAPK & PP2A was evaluated. Immunoprecipitated PP2A activity was measured. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01, vs. control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415363717.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - Characterization of CIP2A expression in a panel of normal human melanocytes (NHM) & melanoma cell lines. (A) Immunoblot analysis & (B) quantification of protein expression levels of CIP2A (C) CIP2A mRNA expression measured by qRT-PCR. NHM1 line was used as calibrator. (D) Cytoplasmic & nuclear expression of CIP2A in normal melanocytes, primary & metastatic melanoma cell lines assessed by immunoblot analysis & (E) by immunohistochemical staining. CIP2A, cancerous inhibitor of protein phosphatase 2A. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25663244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-31020241539738.jpg)
![Western Blot: CIP2A Antibody [NB100-74663] - GAS6 triggered Schwann cell proliferation primarily through CIP2A & DAPK(A) RSC96 cells were transfected with either control or DAPK siRNA for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. Immunoblotting evaluations of pAxl, Axl, CIP2A, DAPK, pERK1/2, ERK1/2, pAKT, AKT, Myc & Survivin. (B) RSC96 cells were transfected with either control or CIP2A siRNA for 48 h & then exposed to GAS6 (100 ng/ml) for 30 min. Immunoblotting evaluations of pAxl, Axl, DAPK, pDAPK, pERK1/2, ERK1/2, pAKT, AKT, Myc & Survivin. (C) DAPK or CIP2A was knocked down for 48 h then RSC96 cells were incubated with GAS6 (100 ng/ml) at the indicated hours. Cell viability was analysed via the WST-1 assay. Data are the mean ± SD, & n = 3 for each time point. * p < 0.05, ** p < 0.01 vs. scramble. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74663_rabbit-polyclonal-cip2a-antibody-310202415532020.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody [NBP1-87102] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining Is shown in green.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87102-img0018.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Western Blot: FOXP1 Antibody (JC12) [NB100-65125] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Western-Blot-NB100-65125-img0003.jpg "Western Blot: FOXP1 Antibody (JC12) [NB100-65125] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: FOXP1 Antibody (JC12) [NB100-65125] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Immunocytochemistry-Immunofluorescence-NB100-65125-img0005.jpg)
![Immunohistochemistry-Paraffin: FOXP1 Antibody (JC12) [NB100-65125] - IHC analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The carcinoma cells developed an expected and specific strong nuclear with mild cytoplasmic immunopositivity for FOXP1 protein.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Immunohistochemistry-Paraffin-NB100-65125-img0015.jpg)
![Flow Cytometry: FOXP1 Antibody (JC12) [NB100-65125] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using FOXP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Flow-Cytometry-NB100-65125-img0011.jpg)
![Immunohistochemistry-Paraffin: FOXP1 Antibody (JC12) [NB100-65125] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The breast ductal/acinar epithelial cells and the myoepithelial cells developed a strong nuclear along with moderate cytoplasmic immuno-positivity for FOXP1 protein.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Immunohistochemistry-Paraffin-NB100-65125-img0012.jpg)
![Immunohistochemistry-Paraffin: FOXP1 Antibody (JC12) [NB100-65125] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal brain using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The cells in the brain tissue depicted strong specific nuclear along with relatively weak cytoplasmic immunopositivity for FOXP1 protein.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Immunohistochemistry-Paraffin-NB100-65125-img0013.jpg)
![Immunohistochemistry-Paraffin: FOXP1 Antibody (JC12) [NB100-65125] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal colon using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. Most of the cells depicted an expected strong nuclear with mild cytoplasmic staining.](http://images.novusbio.com/fullsize/FOXP1-Antibody-JC12-Immunohistochemistry-Paraffin-NB100-65125-img0014.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg "Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0005.jpg)
![Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunohistochemistry-NBP1-48321-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0007.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)
![Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Western-Blot-NB100-206-img0006.jpg "Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.")
![Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-206-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0007.jpg)
![SMC1 [p Ser966] Antibody [NB100-206] - Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody NB100-206 used for IP at 3 ul per reaction. Phospho SMC1 (S966) was also immunoprecipitated by rabbit anti-SMC1 antibody NB100-204. For blotting immunoprecipitated Phospho SMC1 NB100-206 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody NB100-204 was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-NB100-206-img0009.jpg)
![Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-561-img0023.jpg "Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse teratoma Antibody: Affinity purified rabbit anti-FUS (NB100-561) used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-561-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-NB100-561-img0020.jpg)
![Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FUS Antibody [NB100-561] - Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-561 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-565. For blotting immunoprecipitated FUS, NBP2-76415 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0021.jpg)
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Western-Blot-NB100-136-img0005.jpg "Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Immunocytochemistry-Immunofluorescence-NB100-136-img0003.jpg)
![Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) [NB100-136] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using Cited-2 antibody at 1:500 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Staining was localized to the nucleus.](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Immunohistochemistry-Paraffin-NB100-136-img0004.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in human colon.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0015.jpg "Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in human colon.")
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody [H00005888-B01P] - Immunofluorescence of purified MaxPab antibody to RAD51 on HeLa cell. [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunocytochemistry-Immunofluorescence-H00005888-B01P-img0008.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody [H00005888-B01P] - Analysis of purified antibody to RAD51 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-H00005888-B01P-img0010.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - expression in transfected 293T cell line. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0009.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in PC-12.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0011.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0012.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Jurkat.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0013.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0014.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in transfected 293T cell line by RAD51 polyclonal antibody. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0016.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg)
![Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg)
![Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg)
![Western Blot: CYBB/NOX2 Antibody [NBP2-41291] - Analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Western-Blot-NBP2-41291-img0001.jpg "Western Blot: CYBB/NOX2 Antibody [NBP2-41291] - Analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/ml.")
![Immunohistochemistry: CYBB/NOX2 Antibody [NBP2-41291] - NOX2 expression in mouse cerebral cortex. Dilution 1:1000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Immunohistochemistry-NBP2-41291-img0009.jpg)
![Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunohistochemistry of CYBB/NOX2 in rat brain tissue cells with CYBB/NOX2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41291_rabbit-polyclonal-cybb-nox2-antibody-2711202419562149.gif)
![Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunofluorescence of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 20 u/ml.Green: CYBB/NOX2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41291_rabbit-polyclonal-cybb-nox2-antibody-2711202419333437.gif)
![Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunohistochemistry of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41291_rabbit-polyclonal-cybb-nox2-antibody-2711202419251538.jpg)
![Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunofluorescence of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41291_rabbit-polyclonal-cybb-nox2-antibody-2711202420243363.jpg)
