FoxP1 Antibody (JC12) - Azide and BSA Free

Western Blot: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The carcinoma cells developed an expected and specific strong

Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using FOXP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal brain using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The cells in the brain tissue depicted strong specific nuclear along with relatively weak cy

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The breast ductal/acinar epithelial cells and the myoepithelial cells developed a strong nuclear along with

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal colon using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. Most of the cells depicted an expected strong nuclear with mild cytoplasmic staining. Image

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: JC12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

FoxP1 Antibody (JC12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FoxP1 Antibody (JC12) - Azide and BSA Free (NB100-65125) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human FOXP1 [Uniprot# Q9H334]
• Epitope: Within the C-terminus
• Localization: Nuclear, Cytoplasmic
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 15161711)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation reported in scientific literature (PMID 17586580)
  • Western Blot 2ug/ml
• Application Notes: Heat induced antigen retrieval with Sodium Citrate buffer pH 6.0 is recommended when using this antibody for IHC-P. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for FoxP1 Antibody (JC12) - Azide and BSA Free

• FLJ23741
• fork head-related protein like B
• forkhead box P1
• forkhead box protein P1
• FoxP1
• glutamine-rich factor 1
• hFKH1B
• HSPC215
• MGC12942
• MGC88572
• MGC99551
• QRF1
• QRF112CC4

Background

FOXP1 is a member of the Forkhead Box Protein family. These proteins are transcription factors that are characterised by a highly conserved forkhead DNA-binding domain. FOXP1 is a repressor of transcription. It forms homodimers, and also heterodimerises with FOXP2 and FOXP4, to facilitate its binding to DNA for transcriptional regulation. A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukaemia, and FOXP1 is also implicated in mental retardation with language impairment and autistic features (MRLIAF). Chromosomal translocation of FOXP1 is implicated in mucosa-associated lymphoid tissue (MALT) lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXP1