| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47662-0.1mg | |||
| IHC-Prediluted | NBP2-48189 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47858-0.1mg | |||
| IHC-Prediluted | NBP2-48223 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85251-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88606-25ul | |||
![Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-03276-img0002.jpg "Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).")
![Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276] - Staining of human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-03276-img0003.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Western-Blot-NBP2-45274-img0006.jpg "Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Formalin-fixed paraffin-embedded rat stomach stained with muscle specific actin monoclonal antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0007.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0004.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0005.jpg)
![Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin format of this antibody.](http://images.novusbio.com/fullsize/nbp2-45274_mouse-monoclonal-pan-actin-antibody-msa-953-17720249362611.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Immunohistochemistry-Paraffin-NBP2-44607-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-1772024100320.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-177202495943.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.Lane 1: Hela-si NT cell lysate (10 ug/Lane)Lane 2: Hela-si alpha smooth muscle Actin cell lysate (10 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel. was shown to specifically react with alpha smooth muscle Actin in Hela-si NT cells. Weakened band was observed when Hela-si alpha smooth muscle Actin sample was tested. Hela-si NT and Hela-si alpha smooth muscle Actin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67440_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-sy02-64-western-blot-2372024113558..jpg)
![Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: 293 cell lysateLane 4: MCF-7 cell lysate](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Western-Blot-NBP2-66840-img0010.jpg "Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: 293 cell lysateLane 4: MCF-7 cell lysate")
![Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunocytochemistry-Immunofluorescence-NBP2-66840-img0009.jpg)
![Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunohistochemistry-Paraffin-NBP2-66840-img0007.jpg)
![Flow Cytometry: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Flow-Cytometry-NBP2-66840-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunocytochemistry-Immunofluorescence-NBP2-66840-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunocytochemistry-Immunofluorescence-NBP2-66840-img0003.jpg)
![Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunohistochemistry-Paraffin-NBP2-66840-img0004.jpg)
![Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse brain tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunohistochemistry-Paraffin-NBP2-66840-img0005.jpg)
![Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FGFR3-Antibody-JM110-33-Immunohistochemistry-Paraffin-NBP2-66840-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Western Blot: ST2/IL-33 R Antibody [NBP1-85251] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/ST2-IL-33-R-Antibody-Western-Blot-NBP1-85251-img0008.jpg "Western Blot: ST2/IL-33 R Antibody [NBP1-85251] - Analysis in human cell line HEL.")
![Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ST2-IL-33-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85251-img0007.jpg)
![Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human stomach shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/ST2-IL-33-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85251-img0004.jpg)
![Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ST2-IL-33-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85251-img0005.jpg)
![Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/ST2-IL-33-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85251-img0006.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Western Blot of Mouse heart tissue lysate, using NBP2-61474 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-RM257-Western-Blot-NBP2-61474-img0003.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Western Blot of Mouse heart tissue lysate, using NBP2-61474 at a 1:1000 dilution")
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Immunohistochemical staining of FFPE Human heart tissue sections using NBP2-61474 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-RM257-Immunohistochemistry-NBP2-61474-img0002.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Analysis in human placenta and pancreas tissues using NBP1-88606 antibody. Corresponding TGFBI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0031.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Analysis in human placenta and pancreas tissues using NBP1-88606 antibody. Corresponding TGFBI RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal, pancreas, placenta and skin using Anti-Beta Ig-h3/TGFBI antibody NBP1-88606 (A) shows similar protein distribution across tissues to independent antibody NBP2-48534 (B).](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0025.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human placenta shows moderate to strong membranous positivity in stromal cells.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0019.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human eye shows moderate extracellular positivity in cornea.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0026.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal shows moderate positivity in extracellular matrix of glandular cells.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0027.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0028.jpg)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human skin shows moderate positivity in extracellular matrix of dermis.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0029.jpg)
![Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Simple Western lane view shows a specific band for Beta Ig-h3/TGFBI in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Simple-Western-NBP1-88606-img0005.jpg)
![Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Electropherogram image of the corresponding Simple Western lane view. Beta Ig-h3/TGFBI antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Simple-Western-NBP1-88606-img0006.jpg)
![Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Mouse plasma and rat plasma (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Western-Blot-NBP1-32436-img0018.jpg "Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Mouse plasma and rat plasma (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32436-img0007.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436] - Breast cancer. Fibrinogen gamma antibody dilution: 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Immunohistochemistry-Paraffin-NBP1-32436-img0004.jpg)
![Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Western-Blot-NBP1-32436-img0010.jpg)
![Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Human plasma and HepG2 conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Western-Blot-NBP1-32436-img0016.jpg)
![Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma protein from HepG2 whole cell extracts using 5 ug of Fibrinogen gamma antibody. Western blot analysis was performed using Fibrinogen gamma antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-Immunoprecipitation-NBP1-32436-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma chain antibody detects Fibrinogen gamma chain protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Fibrinogen gamma chain stained by Fibrinogen gamma chain antibody (NBP1-32436) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-32436_rabbit-polyclonal-fibrinogen-gamma-chain-antibody-910202420115017.jpg)
