| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76695-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70732 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84978-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26876-25ul | |||
| Azide and BSA Free | NBP3-44299 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54690-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28632SS | |||
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0011.jpg "Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0004.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0010.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK/TNFSF12 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420115594.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK/TNFSF12 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420272466.gif)
![Immunocytochemistry/ Immunofluorescence: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK/TNFSF12 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202419514021.gif)
![Western Blot: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibrinogen gamma chain.](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Western-Blot-NBP2-45663-img0006.jpg "Western Blot: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibrinogen gamma chain.")
![Immunohistochemistry: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Immunohistochemistry-NBP2-45663-img0005.jpg)
![Western Blot: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of extracts (35ug) from 9 different cell lines by using FGG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Western-Blot-NBP2-45663-img0007.jpg)
![Immunohistochemistry: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Immunohistochemistry-NBP2-45663-img0001.jpg)
![Immunohistochemistry: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Immunohistochemistry-NBP2-45663-img0002.jpg)
![Immunohistochemistry: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Immunohistochemistry-NBP2-45663-img0003.jpg)
![Immunohistochemistry: Fibrinogen gamma chain Antibody (2D2) [NBP2-45663] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Fibrinogen-gamma-chain-Antibody-2D2-Immunohistochemistry-NBP2-45663-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (4H11[APG]) [NB500-608] - Human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Immunocytochemistry-Immunofluorescence-NB500-608-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD34 Antibody (4H11[APG]) [NB500-608] - Human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.")
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0010.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Analysis using the FITC conjugate of NB500-608. Staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0002.jpg)
![Flow Cytometry: CD34 Antibody (4H11[APG]) [NB500-608] - Staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 700.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cytometry-NB500-608-img0004.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Pacific BlueTM](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0005.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) PE-CyTM7.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0006.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0007.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) APC.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0008.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (4H11[APG]) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0009.jpg)
![Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Western-Blot-NBP3-11864-img0001.jpg "Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - ICC staining VEGFR1/Flt-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0003.jpg)
![Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Flow-Cytometry-NBP3-11864-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0005.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0002.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Analysis in human heart muscle and liver tissues. Corresponding FHL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Analysis in human heart muscle and liver tissues. Corresponding FHL2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human cerebellum, heart muscle, liver and skeletal muscle using Anti-FHL2 antibody NBP1-84978 (A) shows similar protein distribution across tissues to independent antibody NBP1-84979 (B).](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0020.jpg)
![Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FHL2-Antibody-Western-Blot-NBP1-84978-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FHL2 Antibody [NBP1-84978] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84978-img0009.jpg)
![Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0023.jpg)
![Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/FHL2-Antibody-Western-Blot-NBP1-84978-img0006.jpg)
![Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human cerebellum shows low positivity as expected.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0018.jpg)
![Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human heart muscle shows strong cytoplasmic and nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0021.jpg)
![Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FHL2-Antibody-Immunohistochemistry-Paraffin-NBP1-84978-img0022.jpg)
![Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NBP2-54690-img0009.jpg "Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NBP2-54690-img0008.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0001.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0002.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0003.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0004.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0005.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0008.jpg)
![Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Western-Blot-NBP1-28632-img0005.jpg "Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.")
![Immunocytochemistry/Immunofluorescence: EDIL3/DEL1 Antibody [NBP1-28632] - HeLa cells stained NBP1-28632 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28632-img0004.jpg)
![Simple Western: EDIL3/DEL1 Antibody [NBP1-28632] - Simple Western lane view shows a specific band for EDIL3/DEL1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Simple-Western-NBP1-28632-img0006.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.")
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] - FAK antibody detects FAK protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FAK stained by FAK antibody (NBP3-12897) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-12897_rabbit-fak-p-tyr-397-pab-910202419593568.jpg)
