| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33819-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76454SS | |||
| Azide and BSA Free | NBP2-80767 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48154 | |||
| Azide and BSA Free | NBP2-34634-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91228-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71459 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76695-0.025mg | |||
![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Western blot analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0009.jpg "Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Western blot analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunocytochemistry-Immunofluorescence-NBP2-67067-img0002.jpg)
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0006.jpg)
![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0007.jpg)
![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunocytochemistry-Immunofluorescence-NBP2-67067-img0001.jpg)
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human colon cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0003.jpg)
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human liver cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0004.jpg)
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded mouse orchis tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0005.jpg)
![Western Blot: CXCL8/IL-8 Antibody [NBP2-33819] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Western-Blot-NBP2-33819-img0005.jpg "Western Blot: CXCL8/IL-8 Antibody [NBP2-33819] - Analysis in human cell line EFO-21.")
![Immunocytochemistry/Immunofluorescence: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33819-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human rectum shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0010.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0007.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in a subset of non-germinal center cells.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human placenta shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0009.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0001.jpg "Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Human MDA MB 231 cells. 50 ug cell lysate per lane. 10% SDS-PAGE. HIF-2 alpha antibody at 1:1000 in 5% milk. HRP-conjugated donkey anti-rabbit IgG polyclonal secondary antibody at 1:2000 in 5% milk. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0003.jpg)
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Analysis of an FFPE tissue section of the human renal carcinoma using 1:100 dilution of HIF-2 alpha antibody (NBP2-76454). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the nuclear with minor cytoplasmic staining.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Immunohistochemistry-Paraffin-NBP2-76454-img0002.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0013.jpg "Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Immunocytochemistry-Immunofluorescence-H00001958-M03-img0010.jpg)
![Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Immunohistochemistry-Paraffin-H00001958-M03-img0011.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of EGR1 expression in transfected 293T cell line by EGR1 monoclonal antibody (M03), clone 6E8. Lane 1: EGR1 transfected lysatE (59.73 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0012.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0014.jpg)
![ELISA: EGR1 Antibody (6E8) [H00001958-M03] - Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-ELISA-H00001958-M03-img0006.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0011.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0008.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0004.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0005.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) respectively.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0007.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0008.jpg "Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0006.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin with Rabbit anti-Actin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0009.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0004.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-91228-img0009.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by Western blot. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0013.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0012.jpg)
![Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/HAND1-Antibody-1G10-Western-Blot-NBP2-00576-img0005.jpg "Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).")
![Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.](http://images.novusbio.com/fullsize/HAND1-Antibody-1G10-Immunocytochemistry-Immunofluorescence-NBP2-00576-img0004.jpg)
![Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HAND1-Antibody-1G10-Flow-Cytometry-NBP2-00576-img0002.jpg)
![Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.](http://images.novusbio.com/fullsize/HAND1-Antibody-1G10-Western-Blot-NBP2-00576-img0003.jpg)
![Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HAND1-Antibody-1G10-Flow-Cytometry-NBP2-00576-img0001.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0011.jpg "Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0004.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0010.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK/TNFSF12 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420115594.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK/TNFSF12 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420272466.gif)
![Immunocytochemistry/ Immunofluorescence: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK/TNFSF12 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202419514021.gif)