Zyxin Antibody

Product Discontinued

Zyxin Antibody Summary

• Immunogen: Synthetic peptide conjugated by N-terminal added cysteine to KLH, corresponding to amino acids 134-150 of Human Zyxin.
• Localization: Cytoplasmic
• Marker: Focal Adhesion Marker
• Specificity: Zyxin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZYX
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:100-1:2000
• Application Notes: WB: Detects a band of approximately 82-84 kDa. By immunoblotting, a minimum working antibody dilution of 1:1,000 is recommended using whole extract of human cervix epitheloid carcinoma (HeLa) cells and a chemiluminescent detection reagent. By immunoblotting, a minimum working antibody dilution of 1:2,000 is recommended using whole extract of mouse NIH-3T3 cells and a chemiluminescent detection reagent. By indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recemmended using rat Rat1 cells.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: IgG purified

Alternate Names for Zyxin Antibody

• CTCL tumor antigen se14-3
• CTCL-associated antigen se14-3
• cutaneous T-cell lymphoma associated antigen se14-3
• Cutaneous T-cell lymphoma-associated antigen se14-3
• KIAA1125
• MGC31836
• predicted protein of HQ2893
• PRKCBP1
• PRO2893
• protein kinase C-binding protein 1
• Rack7
• RACK7protein kinase C binding protein 1
• zinc finger MYND domain containing protein 8
• Zinc finger MYND domain-containing protein 8
• zinc finger, MYND-type containing 8
• Zyx
• Zyxin 2
• Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZYX
• Entrez:
  • 7791(Human)
  • 22793(Mouse)
  • 114636(Rat)