Zyxin Antibody (JU83-12)

Western Blot: Zyxin Antibody (JU83-12) [NBP2-75723] - Analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (JU83-12) [NBP2-75723] - Staining Zyxin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Zyxin Antibody (JU83-12) [NBP2-75723] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Zyxin antibody. Counter stained with hematoxylin.

Flow Cytometry: Zyxin Antibody (JU83-12) [NBP2-75723] - Analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (JU83-12) [NBP2-75723] - Staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Zyxin Antibody (JU83-12) [NBP2-75723] - Analysis of paraffin-embedded human kidney tissue using anti-Zyxin antibody. Counter stained with hematoxylin.

Product Discontinued

Zyxin Antibody (JU83-12) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Zyxin aa 284-343 / 572. (SwissProt: Q15942 Human; SwissProt: Q62523 Mouse)
• Localization: Cytoplasm, cytoskeleton and Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ZYX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Zyxin Antibody (JU83-12)

• CTCL tumor antigen se14-3
• CTCL-associated antigen se14-3
• cutaneous T-cell lymphoma associated antigen se14-3
• Cutaneous T-cell lymphoma-associated antigen se14-3
• KIAA1125
• MGC31836
• predicted protein of HQ2893
• PRKCBP1
• PRO2893
• protein kinase C-binding protein 1
• Rack7
• RACK7protein kinase C binding protein 1
• zinc finger MYND domain containing protein 8
• Zinc finger MYND domain-containing protein 8
• zinc finger, MYND-type containing 8
• Zyx
• Zyxin 2
• Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZYX