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ZRANB2 Antibody

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Western Blot: ZRANB2 Antibody [NB100-55322] - Detection of Human ZNF265/ZIS on HeLa whole cell lysate using NB100-55322. Trace amounts of ZNF265/ZIS were also immunoprecipitated by rabbit anti-ZNF265/ZIS antibody NB100-55323.
Independent Antibodies: Western Blot: ZRANB2 Antibody [NB100-55322] - Detection of Human ZNF265/ZIS on HeLa whole cell lysate using NB100-55322. Trace amounts of ZNF265/ZIS were also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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ZRANB2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human zinc-finger protein 265 (zinc-finger, splicing) using the numbering given in entry NP_005446.2 (GeneID 9406).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZRANB2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 5 ug/mg lysate
Positive Control
ZRANB2 Lysate (NBL1-18266)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZRANB2 Antibody

  • Oocyte-specific maternal effect factor
  • zygote arrest 1
  • zygote arrest protein 1

Background

ZNF265 is an SR (serine/arginine-rich) domain containing zinc-finger protein and a component of the splicesome. ZNF265 interacts with U1-70K and U2AF35 and functions in directing alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Renin was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Renin was detected in immersion fixed paraffin-embedded sections of human kidney array using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
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Applications: WB, IHC, IP, Neut
gamma-Synuclein
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NBP2-66861
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Staining gamma Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of paraffin-embedded rat bladder tissue using anti-gamma Synuclein antibody. Counter stained with hematoxylin.
Flow Cytometry: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of Hela cells with gamma Synuclein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Secretin R Antibody
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Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human Secretin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6387) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Secretin R delta 3/4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
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Western Blot: RNPS1 Antibody [NBP1-92343] - Analysis in human cell line RT-4
Immunocytochemistry/Immunofluorescence: RNPS1 Antibody [NBP1-92343] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RNPS1 Antibody [NBP1-92343] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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H00008227-M02
Western Blot: XE7 Antibody (2G8) [H00008227-M02] - SFRS17A monoclonal antibody (M02), clone 2G8 Analysis of SFRS17A expression in Hela S3 NE.
Immunocytochemistry/Immunofluorescence: XE7 Antibody (2G8) [H00008227-M02] - Analysis of monoclonal antibody to SFRS17A on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: XE7 Antibody (2G8) [H00008227-M02] - Analysis of monoclonal antibody to SFRS17A on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
SNRP70 Antibody
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Western Blot: SNRP70 Antibody [NBP1-57487] - Antibody Titration: 1 ug/ml HEK293.
Immunohistochemistry-Paraffin: SNRP70 Antibody [NBP1-57487] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: SNRP70 Antibody [NBP1-57487] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
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Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
SMN2 Antibody
(2B11-2A9)
H00006607-M01
Western Blot: SMN2 Antibody (2B11-2A9) [H00006607-M01] - SMN2 monoclonal antibody (M01), clone 2B11-2A9 Analysis of SMN2 expression in IMR-32.
Immunocytochemistry/Immunofluorescence: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 1 ~ 10 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
U2AF35 Antibody
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Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
KAT3B/p300
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Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-KAT3B/p300 (RW105) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
(8 Publications)
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
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Western Blot: YY1 Antibody [NBP1-46218] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-YY1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. YY1 was also immunoprecipitated by rabbit anti-YY1 antibody NBP1-46217, which recognizes an upstream epitope.
Immunohistochemistry: YY1 Antibody [NBP1-46218] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-YY1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Proximity Ligation Assay: YY1 Antibody [NBP1-46218] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human YY1 (NBP1-46218). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
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Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP1-90821] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Proteasome 19S S5A Antibody [NBP1-90821] - Staining in human testis and pancreas tissues using anti-PSMD4 antibody. Corresponding PSMD4 RNA-seq data are presented for the same tissues.
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EphA3
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EphA3
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Western Blot: RPL31 Antibody [NBP2-58847] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: RPL31 Antibody [NBP2-58847] - Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
Species: Hu
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SC35 Antibody
(SC-35)
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Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:100; 2. Antibody dilution 1:250; 3. Antibody dilution 1:500; 4. Antibody dilution 1:1,000
Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing Factor Clone: SC-35 diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, FITC-conjugate.
(5 Publications)
Species: Hu, Rt, Am, Dr
Applications: WB, EM, ELISA, ICC/IF, IP
ARSF Antibody
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Western Blot: ARSF Antibody [NBP1-86191] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: ARSF Antibody [NBP1-86191] - Staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: ARSF Antibody [NBP1-86191] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF
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(2D2)
H00006428-M08
Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/Immunofluorescence: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of monoclonal antibody to SFRS3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in HeLa (Cat # L013V1).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
ZRANB2 Antibody
NB100-55322
ZRANB2 Antibody
Species: Hu
Applications: WB, IP

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Positive Control Lysate(s)

ZRANB2 Overexpression Lysate (Native)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZRANB2
Entrez
Uniprot