ZNF703 Antibody (CL0654) - BSA Free

Western Blot: ZNF703 Antibody (CL0654) [NBP2-52940] - Lane1: Marker [KDa] Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: ZNF703 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (-3.1 KDa) in mammalian HEK293T cells, LY410937).

Immunohistochemistry-Paraffin: ZNF703 Antibody (CL0654) [NBP2-52940] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Western Blot: ZNF703 Antibody (CL0654) [NBP2-52940] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ZNF703 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry-Paraffin: ZNF703 Antibody (CL0654) [NBP2-52940] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: ZNF703 Antibody (CL0654) [NBP2-52940] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: ZNF703 Antibody (CL0654) [NBP2-52940] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ZNF703 Antibody (CL0654) [NBP2-52940] - Staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, Mycoplasma
• Clone: CL0654
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ZNF703 Antibody (CL0654) - BSA Free Summary

• Description: Novus Biologicals Mouse ZNF703 Antibody (CL0654) - BSA Free (NBP2-52940) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9H7S9, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF703
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for ZNF703 Antibody (CL0654) - BSA Free

• FLJ14299
• ZEPPO1
• Zinc finger elbow-related proline domain protein 1
• zinc finger protein 703
• ZNF503L
• ZPO1

Background

ZNF703 interacts selectively with zinc (Zn) ions. May function as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF703